Click here to switch to the map view.

The map label for this gene is tfb [H]

Identifier: 124027349

GI number: 124027349

Start: 441277

End: 442170

Strand: Direct

Name: tfb [H]

Synonym: Hbut_0458

Alternate gene names: 124027349

Gene position: 441277-442170 (Clockwise)

Preceding gene: 124027348

Following gene: 124027350

Centisome position: 26.47

GC content: 50.11

Gene sequence:

>894_bases
ATGAGTATACCTCTACCCAAGGGTCTGGAATATTGTCCCGTGTGTAAGGCGCCCCTAAAGCTCTACGCTAAACGTGATGG
GAGTAAGCTAGTCTGCACCAACTGTGGCTACGTGATAGACGATGCACCACTAGACACTGGACCCGAGTGGAGGAGCTATA
CTGAAGAGGATAGGCTGCTTAGGAGCAGGGTTGGCGCACCTTTAACCGAGCGGGTACACGATAAAGGCTTGACAACATAT
GTCCAGCGGAACCGCCGAGACCCACGTGCAATGAAGATTGTGCAGCTACAAACACAGCTTAGAACCCACGGCCAAAAGAA
GATGATTAAGCTCCTGCAGGACCTCAATCACTTTGTCGCAAAGCTTAATCTCCCACCACGCGTCGCCGAGACCATGGCTA
AGCTCGTGAAGAAGCTATACCTTATGGGCGTTATCAAGAAGAATAATGAGCATGTCTACCTTGCAGCTGCAGCAGTCATA
GCTTCAAGGATAGAGGGGCACTCACTAACAATGCGCGATGTTGCCGAGACTCTCGGACTGAGCAGGCAGGAGATTTGGAA
GGCTTACAGGAAGATAGTGACAAAGCTCAAGGTGAGAGTTGCAACTCCACCCAGACCACAAATGTACGTATCAAAGATAG
CGTCAAAGCTCAAGCTTTCTGGCGAGGTTGAGGCATTAGCTACCCGATTCACAATAATGCTAACCAAGACCGGTATTGCG
CAGGGCAAGCCGCCGGAAGCTCTGGCAGCTGCAGCCGTCTACGTGGCATCCATACTACTCGACGAGAAAAGGAACCAGCA
GAGAGTCGCACAAGCAATAGGAGTTACGGATGCAACAATAAGGAACAGGTATAGAGATATCGTAGACAACTTCTACATTG
AGGTTAGGCTTTAG

Upstream 100 bases:

>100_bases
GCAGGAGGCCGCGCAAAACGAGAACTCCAAGACAACACCGTGGTAGACAGGCTGGCCGGAGGGGTGGCGGGGGTGGTAGG
GGGAGGGGAGGTAGGAGGAG

Downstream 100 bases:

>100_bases
CTAGACTTCTTTCTCCCTTTCTTCCAAACCTGTCTAGCCACCACCACATATTTCATACTGGCTGCCCACTTTACCGCCTT
CAACGGTTTACGGTAGTGTG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSIPLPKGLEYCPVCKAPLKLYAKRDGSKLVCTNCGYVIDDAPLDTGPEWRSYTEEDRLLRSRVGAPLTERVHDKGLTTY
VQRNRRDPRAMKIVQLQTQLRTHGQKKMIKLLQDLNHFVAKLNLPPRVAETMAKLVKKLYLMGVIKKNNEHVYLAAAAVI
ASRIEGHSLTMRDVAETLGLSRQEIWKAYRKIVTKLKVRVATPPRPQMYVSKIASKLKLSGEVEALATRFTIMLTKTGIA
QGKPPEALAAAAVYVASILLDEKRNQQRVAQAIGVTDATIRNRYRDIVDNFYIEVRL

Sequences:

>Translated_297_residues
MSIPLPKGLEYCPVCKAPLKLYAKRDGSKLVCTNCGYVIDDAPLDTGPEWRSYTEEDRLLRSRVGAPLTERVHDKGLTTY
VQRNRRDPRAMKIVQLQTQLRTHGQKKMIKLLQDLNHFVAKLNLPPRVAETMAKLVKKLYLMGVIKKNNEHVYLAAAAVI
ASRIEGHSLTMRDVAETLGLSRQEIWKAYRKIVTKLKVRVATPPRPQMYVSKIASKLKLSGEVEALATRFTIMLTKTGIA
QGKPPEALAAAAVYVASILLDEKRNQQRVAQAIGVTDATIRNRYRDIVDNFYIEVRL
>Mature_296_residues
SIPLPKGLEYCPVCKAPLKLYAKRDGSKLVCTNCGYVIDDAPLDTGPEWRSYTEEDRLLRSRVGAPLTERVHDKGLTTYV
QRNRRDPRAMKIVQLQTQLRTHGQKKMIKLLQDLNHFVAKLNLPPRVAETMAKLVKKLYLMGVIKKNNEHVYLAAAAVIA
SRIEGHSLTMRDVAETLGLSRQEIWKAYRKIVTKLKVRVATPPRPQMYVSKIASKLKLSGEVEALATRFTIMLTKTGIAQ
GKPPEALAAAAVYVASILLDEKRNQQRVAQAIGVTDATIRNRYRDIVDNFYIEVRL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=261, Percent_Identity=26.4367816091954, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17564892, Length=287, Percent_Identity=24.390243902439, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17136758, Length=260, Percent_Identity=24.6153846153846, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 33505; Mature: 33374

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPLPKGLEYCPVCKAPLKLYAKRDGSKLVCTNCGYVIDDAPLDTGPEWRSYTEEDRLL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHCCCHHHHHH
RSRVGAPLTERVHDKGLTTYVQRNRRDPRAMKIVQLQTQLRTHGQKKMIKLLQDLNHFVA
HHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KLNLPPRVAETMAKLVKKLYLMGVIKKNNEHVYLAAAAVIASRIEGHSLTMRDVAETLGL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCC
SRQEIWKAYRKIVTKLKVRVATPPRPQMYVSKIASKLKLSGEVEALATRFTIMLTKTGIA
CHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCC
QGKPPEALAAAAVYVASILLDEKRNQQRVAQAIGVTDATIRNRYRDIVDNFYIEVRL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEC
>Mature Secondary Structure 
SIPLPKGLEYCPVCKAPLKLYAKRDGSKLVCTNCGYVIDDAPLDTGPEWRSYTEEDRLL
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHCCCHHHHHH
RSRVGAPLTERVHDKGLTTYVQRNRRDPRAMKIVQLQTQLRTHGQKKMIKLLQDLNHFVA
HHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KLNLPPRVAETMAKLVKKLYLMGVIKKNNEHVYLAAAAVIASRIEGHSLTMRDVAETLGL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCC
SRQEIWKAYRKIVTKLKVRVATPPRPQMYVSKIASKLKLSGEVEALATRFTIMLTKTGIA
CHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCC
QGKPPEALAAAAVYVASILLDEKRNQQRVAQAIGVTDATIRNRYRDIVDNFYIEVRL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]