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The map label for this gene is rsmB [H]

Identifier: 124027288

GI number: 124027288

Start: 381952

End: 383223

Strand: Direct

Name: rsmB [H]

Synonym: Hbut_0396

Alternate gene names: 124027288

Gene position: 381952-383223 (Clockwise)

Preceding gene: 124027285

Following gene: 124027290

Centisome position: 22.91

GC content: 56.76

Gene sequence:

>1272_bases
GTGGTGGAGCCCGTTGAGGCTGTATGTGCAAGGATTCTGGCGCGTATCGAGGCTAAACTCTACTCCCTCCGCTACATCGT
CGAGAAGATGAAGCCTCGAGTGCCAAGGGGTCTCCTCAACGCGCTCTGCCTGGGTGTGCTGAGGAACTACAGGCTGCTCA
TACGCGCTCTCCGCTATTGCGGATACCGTGGAGACGTCCGCGGCAATGCCAGAGGCTGGCTTCCAATAGTCATCGCCTAC
GAGGCTATGTTCCGCCACGATTCTGTGCCAGCTGAGAAGCTTAGAAGCCTTGGGGTACTAGGTGGGGAAACCATAGATTG
CTTGCGTGGCCTTGAGCCAAAGCATGTAGTCTCTGGTCTCCATGGGCTCGAGCGACTAGCAGTGCTCTACAGCATGCCGC
TGTGGGTTGTCGAGGAGCTTGCAAAAGCAGAGCCACCAGGCGGCATAGAGGAGTTGCTGAAATCGTTCCAGGAGCCGACA
CCGATATGGATAAGGTTTAACAAGCGTAGGCTCAGTAGGGAGCAAGCCATCAAGATGCTGGAGGCTATTGGCATAAGCGT
GAAGCCAGACCCCGTGCTCGACGACTCGCTAGAGGTTATACGGGTAGAGCCAGGAGCTATAGAGAGGCTTGATAACAGAC
TGTTCTACGTCCAGGATAGAGCTGCGATGCTGGCTTCACACCTCCTAGGGAAGTTCGAGGGTGCCGTGTACGATTTCTTC
AGCGCACCCGGCAATAAGCTAGCACACACCTACTGGCGGAGCGGCCCAACCTACGCTGTAGCACTTGAGGTTTCAAGGCG
GAGGCTACACGACGAGCAGAGGCTCCTGCAGCGCCAGGCAGTACTACTTGTTGACGCTGCCGAGGCTGATGCCACTAGGC
CACCACTGAGGCCGGGCAGCGGGAGGGCTATAATAGTGGATCCAGACTGCACCAGCATGGGCAGGCTGGGTCATAGTCCA
GAGACGAGGCTCTTCCTCGAGAGGGCTGGACGTAGGATCGTTGAAAGACTCGCGGAGCTGCAGGAAGCGGGGCTCAGGGC
AGCACTTAGAACAGCTGCCAGGGGGGCCAAGGTCGTGTATATGACATGTACATTAACGCTCAGGGAGAACGAGGAGGTTG
TTAGGAAGGTTGTTGAGGATGGGCTTGCAGAACTCGTGGAGGCTAAGCCCCTCATAGGCGTATGGAGCCCCTTCATGAGG
AAGACACAGCGCGTATACCCCCACATGTCTAAGTGTACGGGTGGCTTTGCAGCACTACTCATCAGAGTCTAG

Upstream 100 bases:

>100_bases
ACATGGCCTAGAAGCCCTTAATACTATTAGCGTAGCTGTAGCTCGCCACCAACATTCGGAGCCATTGGATCAGCATCGCT
GCATACTGAGGTGTCCATCT

Downstream 100 bases:

>100_bases
AGCGCCTCTATGCTCGGAGTACTTTATTGCTGCGAGTACACGTACAGCAATCTCCCTTGACAGCTTATCGATAGGCTTCT
CTACACCATTCCTAGATCCT

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MVEPVEAVCARILARIEAKLYSLRYIVEKMKPRVPRGLLNALCLGVLRNYRLLIRALRYCGYRGDVRGNARGWLPIVIAY
EAMFRHDSVPAEKLRSLGVLGGETIDCLRGLEPKHVVSGLHGLERLAVLYSMPLWVVEELAKAEPPGGIEELLKSFQEPT
PIWIRFNKRRLSREQAIKMLEAIGISVKPDPVLDDSLEVIRVEPGAIERLDNRLFYVQDRAAMLASHLLGKFEGAVYDFF
SAPGNKLAHTYWRSGPTYAVALEVSRRRLHDEQRLLQRQAVLLVDAAEADATRPPLRPGSGRAIIVDPDCTSMGRLGHSP
ETRLFLERAGRRIVERLAELQEAGLRAALRTAARGAKVVYMTCTLTLRENEEVVRKVVEDGLAELVEAKPLIGVWSPFMR
KTQRVYPHMSKCTGGFAALLIRV

Sequences:

>Translated_423_residues
MVEPVEAVCARILARIEAKLYSLRYIVEKMKPRVPRGLLNALCLGVLRNYRLLIRALRYCGYRGDVRGNARGWLPIVIAY
EAMFRHDSVPAEKLRSLGVLGGETIDCLRGLEPKHVVSGLHGLERLAVLYSMPLWVVEELAKAEPPGGIEELLKSFQEPT
PIWIRFNKRRLSREQAIKMLEAIGISVKPDPVLDDSLEVIRVEPGAIERLDNRLFYVQDRAAMLASHLLGKFEGAVYDFF
SAPGNKLAHTYWRSGPTYAVALEVSRRRLHDEQRLLQRQAVLLVDAAEADATRPPLRPGSGRAIIVDPDCTSMGRLGHSP
ETRLFLERAGRRIVERLAELQEAGLRAALRTAARGAKVVYMTCTLTLRENEEVVRKVVEDGLAELVEAKPLIGVWSPFMR
KTQRVYPHMSKCTGGFAALLIRV
>Mature_423_residues
MVEPVEAVCARILARIEAKLYSLRYIVEKMKPRVPRGLLNALCLGVLRNYRLLIRALRYCGYRGDVRGNARGWLPIVIAY
EAMFRHDSVPAEKLRSLGVLGGETIDCLRGLEPKHVVSGLHGLERLAVLYSMPLWVVEELAKAEPPGGIEELLKSFQEPT
PIWIRFNKRRLSREQAIKMLEAIGISVKPDPVLDDSLEVIRVEPGAIERLDNRLFYVQDRAAMLASHLLGKFEGAVYDFF
SAPGNKLAHTYWRSGPTYAVALEVSRRRLHDEQRLLQRQAVLLVDAAEADATRPPLRPGSGRAIIVDPDCTSMGRLGHSP
ETRLFLERAGRRIVERLAELQEAGLRAALRTAARGAKVVYMTCTLTLRENEEVVRKVVEDGLAELVEAKPLIGVWSPFMR
KTQRVYPHMSKCTGGFAALLIRV

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Escherichia coli, GI2367212, Length=267, Percent_Identity=25.0936329588015, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47497; Mature: 47497

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEPVEAVCARILARIEAKLYSLRYIVEKMKPRVPRGLLNALCLGVLRNYRLLIRALRYC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GYRGDVRGNARGWLPIVIAYEAMFRHDSVPAEKLRSLGVLGGETIDCLRGLEPKHVVSGL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHH
HGLERLAVLYSMPLWVVEELAKAEPPGGIEELLKSFQEPTPIWIRFNKRRLSREQAIKML
HHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
EAIGISVKPDPVLDDSLEVIRVEPGAIERLDNRLFYVQDRAAMLASHLLGKFEGAVYDFF
HHHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SAPGNKLAHTYWRSGPTYAVALEVSRRRLHDEQRLLQRQAVLLVDAAEADATRPPLRPGS
CCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCC
GRAIIVDPDCTSMGRLGHSPETRLFLERAGRRIVERLAELQEAGLRAALRTAARGAKVVY
CCEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MTCTLTLRENEEVVRKVVEDGLAELVEAKPLIGVWSPFMRKTQRVYPHMSKCTGGFAALL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
IRV
CCC
>Mature Secondary Structure
MVEPVEAVCARILARIEAKLYSLRYIVEKMKPRVPRGLLNALCLGVLRNYRLLIRALRYC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GYRGDVRGNARGWLPIVIAYEAMFRHDSVPAEKLRSLGVLGGETIDCLRGLEPKHVVSGL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHH
HGLERLAVLYSMPLWVVEELAKAEPPGGIEELLKSFQEPTPIWIRFNKRRLSREQAIKML
HHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
EAIGISVKPDPVLDDSLEVIRVEPGAIERLDNRLFYVQDRAAMLASHLLGKFEGAVYDFF
HHHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SAPGNKLAHTYWRSGPTYAVALEVSRRRLHDEQRLLQRQAVLLVDAAEADATRPPLRPGS
CCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCC
GRAIIVDPDCTSMGRLGHSPETRLFLERAGRRIVERLAELQEAGLRAALRTAARGAKVVY
CCEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MTCTLTLRENEEVVRKVVEDGLAELVEAKPLIGVWSPFMRKTQRVYPHMSKCTGGFAALL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
IRV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]