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The map label for this gene is speE [H]

Identifier: 124027278

GI number: 124027278

Start: 372529

End: 373446

Strand: Direct

Name: speE [H]

Synonym: Hbut_0383

Alternate gene names: 124027278

Gene position: 372529-373446 (Clockwise)

Preceding gene: 124027277

Following gene: 124027280

Centisome position: 22.35

GC content: 52.94

Gene sequence:

>918_bases
TTGGCTCAGCGGCTGCCCTGGAGGTTTGTAGCTGAGTGGACCAGTGAGGGCGAGATGGTTTTAAGGGAGGTAAAGCGCAT
CTACGCTGCTGGTGCTACGCGGTACCAGGAGTACATGATAGCAGAACTTGCCGGCATTGGCAAAGCATTAGTGCTCGACG
GTAAGGTTCAAAGCAGTATTCTTGACGAGCACTGGTACCACGAGGCGCTCGTCCACCCCATACTACTAGCCCACCAGTGC
CCAAGGAAGGTGCTGGTTATAGGGGGTGGCGAGGGAGCAACTGTTAGGGAGGTTCTAAGGCATAGCTGTGTCGAGCACGT
AACAATGGTTGACATCGATGAGGAGCTTGTAGAGCTTGCAAAGAAGCACCTCGCCGAGTGGCACCAGGGAGCATTTAGCA
GCGATAAGCTAGAACTGGTTATCGGTGATGGTCGCCGCTACGTCGAGAACTGCCACAGAAAGTACGACGCCATAATACTT
GATCTGGTAGACCCCATGGAGGGTGGGCCCGCAGCACGCCTATACACGCTAGAGTTTTACCGTGCTGTCAAGGGCTGCCT
GCGGCCTGGCGGTGCAGTAGTTACACAAGCTACTTCGCCAACACTGTCACCGAGAGTTTACGCTGTTATACGTAACACGT
TAGCTAAAGTATTCACTATTGTGAGACCGTATGTCTCCTATGTGAGGAGCTATAATGGTCTATGGGGCTTTGTAGCTGCT
TCCGACACCGTGGACCCAGCTAAGCTATCAGCTAAGGAGGTTGATGAGCTCATAGCAGCAAGGATAAGGGGACAGCTACG
GTTCTACGATGGCGAGACACATGAGTGGATGTTTAGGCTACCACTACCGGTTAGACAAGTGCTAAGCGAAACCAGGGACT
ATGCAACCGACGAAAAACCCGTCTACGTCCCTGTCTAG

Upstream 100 bases:

>100_bases
GAGCCCAGCATTATTACCGCTAGCTGAGGCTTATAGACCCCTAGCTACGGGCTAGACCACGGCAAGTCTTAACACTACAT
GCATGTATGTGGTGGGGTGT

Downstream 100 bases:

>100_bases
GCGACCGAGACCCAATAAGGTTAAGCCTATAAACCCAGCATAGAGTACTAGCTATCATCATGGCGTGTGGGTGCCGGGGT
GGCCGAGCGGTCCAAGGCGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQC
PRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIIL
DLVDPMEGGPAARLYTLEFYRAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAA
SDTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKPVYVPV

Sequences:

>Translated_305_residues
MAQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQC
PRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIIL
DLVDPMEGGPAARLYTLEFYRAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAA
SDTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKPVYVPV
>Mature_304_residues
AQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCP
RKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIILD
LVDPMEGGPAARLYTLEFYRAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAAS
DTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKPVYVPV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=276, Percent_Identity=31.5217391304348, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1786312, Length=261, Percent_Identity=35.632183908046, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25154106, Length=279, Percent_Identity=35.84229390681, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI212645616, Length=231, Percent_Identity=35.9307359307359, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323175, Length=292, Percent_Identity=31.5068493150685, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=30.3249097472924, Blast_Score=138, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24645443, Length=291, Percent_Identity=34.7079037800687, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21355941, Length=213, Percent_Identity=37.0892018779343, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34437; Mature: 34306

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSI
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
LDEHWYHEALVHPILLAHQCPRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIILDLVDPMEGGPAARLYTLEFY
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEHHCCCCCCCCCEEHHHHHH
RAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAA
HHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
SDTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKP
CCCCCHHHCCHHHHHHHHHHHHHCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
VYVPV
EEECC
>Mature Secondary Structure 
AQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSI
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
LDEHWYHEALVHPILLAHQCPRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIILDLVDPMEGGPAARLYTLEFY
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEHHCCCCCCCCCEEHHHHHH
RAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAA
HHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
SDTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKP
CCCCCHHHCCHHHHHHHHHHHHHCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
VYVPV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]