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The map label for this gene is argG

Identifier: 124027202

GI number: 124027202

Start: 293414

End: 294604

Strand: Reverse

Name: argG

Synonym: Hbut_0305

Alternate gene names: 124027202

Gene position: 294604-293414 (Counterclockwise)

Preceding gene: 124027204

Following gene: 124027201

Centisome position: 17.67

GC content: 57.51

Gene sequence:

>1191_bases
TTGAAGGTTGTGCTCGCATACTCGGGCGGCCTTGACACATCAGCTATACTCGTGCTTCTCCGCGAGCAAGGCCATGAAGT
TGTCACAGTGACTGTCTCGGTTGGCCAGGAGGAGGACCTGGAGGAGGTTGAGGAGAGAGCCTACAAGCTTGGCGCCTCCA
AGCACTACACTGTTAATGCTGTGGAAGAGTTCGCGGAGAAGTACATATCCATGGCTATAAAGGCTAACGCCCTCTACGAG
GACAAGTATCCTCTCGGCACGGCACTTGCACGCCCCCTCATAGCTGAGAAGGTCGCGGAGATAGCTAGGAAGGAGAGTGC
TGATGCCGTAGCCCACGGCTGCACCTCTAAGGGTAACGACCAGGTGAGGTTTGACACTATGCTGAAATACTATCTTGGCG
ATGACATCAAGATTATTGCGCCGGTTCGCGAGCTCAGACTCACCCGCGCCAAGGCGGCCGAGATACTGAGGAGGAACGGG
TTCCAGCCACCAGGACTCCACAAGACCTATAGCATCGACGAGAACTTGTGGAGTAGAAGCATCGAGGGAGGCCCACTCGA
CGACCCCATGGCTGAGCCTCCGGAGGACGCGTTTGCGTGGACTGTCGCGCCCGAGAAGGCTCCCGATCAGCCCCTCTACC
TCGAGATAGGCTTCGAGAAGGGTATACCGGTCTCGATTAATGGGGAAAAGATGCACCTAGCCAAGATAGTATCGCTCCTC
AACAGGCTGGTAGGCGCGTACGGCTACGGCAGGATAGACCACATAGAGAACCGCGTAGTCGGGCTCAAGAGCAGGGAGGT
CTACGAGGCCCCGGCAGCGCTTACACTGATAGAGGCCCATAGGGACCTCGAGAAGACCGTGTACACGCCCAGGGAGCTAA
GGTTCAAGCGGATCCTAGACCAGGAGTGGAGCGACCTCGTATACCAGGGCTTGTGGGTGGAGCCCCTGCGAGCCACCCTT
GAGAAGGCTATAGACGAGCTAAACCGCTGGGTGACAGGAACAGTCAGGGTCAAGGTGTACAAGGGTAGCCTCATGGTTGT
AGGCCGCTGGAGCCCATACAGTGGCTACAGCCGCGAAGCCATAGACTACAACCATGGTTGGTATCCGAGCGACGAGGAGG
CCCGCGGCTTCATAACCATATGGAGCCTTCACAGTCTTGCTGCAGCCAAGGCGCGCAGCCTCTGGGGCTAA

Upstream 100 bases:

>100_bases
AACAGATTCAAGAGAGATATTTCTTCATACGCAATGAATGGTGCATGTCCCTTAACTCCCGGTACCGGGGCCCTCCAGCG
CTGTAGGGTGTACGTCTGGA

Downstream 100 bases:

>100_bases
CCTGGCTGCAATGCCTGCTGGTGGTCTGCGGGTTCAGTAAGATTGATAATAGGACGGTACGCCGCGAGGGTGGTGGGTTA
TTGCTTAGGTACCGAGAGAC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKVVLAYSGGLDTSAILVLLREQGHEVVTVTVSVGQEEDLEEVEERAYKLGASKHYTVNAVEEFAEKYISMAIKANALYE
DKYPLGTALARPLIAEKVAEIARKESADAVAHGCTSKGNDQVRFDTMLKYYLGDDIKIIAPVRELRLTRAKAAEILRRNG
FQPPGLHKTYSIDENLWSRSIEGGPLDDPMAEPPEDAFAWTVAPEKAPDQPLYLEIGFEKGIPVSINGEKMHLAKIVSLL
NRLVGAYGYGRIDHIENRVVGLKSREVYEAPAALTLIEAHRDLEKTVYTPRELRFKRILDQEWSDLVYQGLWVEPLRATL
EKAIDELNRWVTGTVRVKVYKGSLMVVGRWSPYSGYSREAIDYNHGWYPSDEEARGFITIWSLHSLAAAKARSLWG

Sequences:

>Translated_396_residues
MKVVLAYSGGLDTSAILVLLREQGHEVVTVTVSVGQEEDLEEVEERAYKLGASKHYTVNAVEEFAEKYISMAIKANALYE
DKYPLGTALARPLIAEKVAEIARKESADAVAHGCTSKGNDQVRFDTMLKYYLGDDIKIIAPVRELRLTRAKAAEILRRNG
FQPPGLHKTYSIDENLWSRSIEGGPLDDPMAEPPEDAFAWTVAPEKAPDQPLYLEIGFEKGIPVSINGEKMHLAKIVSLL
NRLVGAYGYGRIDHIENRVVGLKSREVYEAPAALTLIEAHRDLEKTVYTPRELRFKRILDQEWSDLVYQGLWVEPLRATL
EKAIDELNRWVTGTVRVKVYKGSLMVVGRWSPYSGYSREAIDYNHGWYPSDEEARGFITIWSLHSLAAAKARSLWG
>Mature_396_residues
MKVVLAYSGGLDTSAILVLLREQGHEVVTVTVSVGQEEDLEEVEERAYKLGASKHYTVNAVEEFAEKYISMAIKANALYE
DKYPLGTALARPLIAEKVAEIARKESADAVAHGCTSKGNDQVRFDTMLKYYLGDDIKIIAPVRELRLTRAKAAEILRRNG
FQPPGLHKTYSIDENLWSRSIEGGPLDDPMAEPPEDAFAWTVAPEKAPDQPLYLEIGFEKGIPVSINGEKMHLAKIVSLL
NRLVGAYGYGRIDHIENRVVGLKSREVYEAPAALTLIEAHRDLEKTVYTPRELRFKRILDQEWSDLVYQGLWVEPLRATL
EKAIDELNRWVTGTVRVKVYKGSLMVVGRWSPYSGYSREAIDYNHGWYPSDEEARGFITIWSLHSLAAAKARSLWG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=394, Percent_Identity=43.4010152284264, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI16950633, Length=394, Percent_Identity=43.4010152284264, Blast_Score=267, Evalue=1e-71,
Organism=Escherichia coli, GI1789563, Length=369, Percent_Identity=27.6422764227642, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324514, Length=403, Percent_Identity=38.7096774193548, Blast_Score=248, Evalue=9e-67,
Organism=Drosophila melanogaster, GI21358151, Length=395, Percent_Identity=38.4810126582278, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_HYPBU (A2BJL6)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012522.1
- ProteinModelPortal:   A2BJL6
- SMR:   A2BJL6
- STRING:   A2BJL6
- GeneID:   4782292
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0305
- eggNOG:   arNOG04108
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   A2BJL6
- ProtClustDB:   PRK00509
- BioCyc:   HBUT415426:HBUT_0305-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44521; Mature: 44521

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 83-83 BINDING 113-113 BINDING 115-115 BINDING 119-119 BINDING 119-119 BINDING 120-120 BINDING 123-123 BINDING 171-171 BINDING 180-180 BINDING 256-256 BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLAYSGGLDTSAILVLLREQGHEVVTVTVSVGQEEDLEEVEERAYKLGASKHYTVNA
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEHHH
VEEFAEKYISMAIKANALYEDKYPLGTALARPLIAEKVAEIARKESADAVAHGCTSKGND
HHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
QVRFDTMLKYYLGDDIKIIAPVRELRLTRAKAAEILRRNGFQPPGLHKTYSIDENLWSRS
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCC
IEGGPLDDPMAEPPEDAFAWTVAPEKAPDQPLYLEIGFEKGIPVSINGEKMHLAKIVSLL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
NRLVGAYGYGRIDHIENRVVGLKSREVYEAPAALTLIEAHRDLEKTVYTPRELRFKRILD
HHHHHHCCCCCHHHHHHHEECCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QEWSDLVYQGLWVEPLRATLEKAIDELNRWVTGTVRVKVYKGSLMVVGRWSPYSGYSREA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCEEEEECCCCCCCCCCCC
IDYNHGWYPSDEEARGFITIWSLHSLAAAKARSLWG
EECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVLAYSGGLDTSAILVLLREQGHEVVTVTVSVGQEEDLEEVEERAYKLGASKHYTVNA
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEHHH
VEEFAEKYISMAIKANALYEDKYPLGTALARPLIAEKVAEIARKESADAVAHGCTSKGND
HHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
QVRFDTMLKYYLGDDIKIIAPVRELRLTRAKAAEILRRNGFQPPGLHKTYSIDENLWSRS
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCC
IEGGPLDDPMAEPPEDAFAWTVAPEKAPDQPLYLEIGFEKGIPVSINGEKMHLAKIVSLL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
NRLVGAYGYGRIDHIENRVVGLKSREVYEAPAALTLIEAHRDLEKTVYTPRELRFKRILD
HHHHHHCCCCCHHHHHHHEECCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QEWSDLVYQGLWVEPLRATLEKAIDELNRWVTGTVRVKVYKGSLMVVGRWSPYSGYSREA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCEEEEECCCCCCCCCCCC
IDYNHGWYPSDEEARGFITIWSLHSLAAAKARSLWG
EECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA