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The map label for this gene is pyrE

Identifier: 124027011

GI number: 124027011

Start: 99471

End: 100076

Strand: Reverse

Name: pyrE

Synonym: Hbut_0111

Alternate gene names: 124027011

Gene position: 100076-99471 (Counterclockwise)

Preceding gene: 124027012

Following gene: 124027010

Centisome position: 6.0

GC content: 52.15

Gene sequence:

>606_bases
GTGAACAGCTATGCACTAGATCTAGCTGATGTGCTTGTCGAGGCTGGTGCGATAAGGTTTGGAGAGTTTAGACTCTCCAG
CGGCTCCACAAGCCCTATCTACATCGATATGAGAATTGTGCCTTCGAAGCCACAACTGTTCCGCCGTGTACTAGGCATGC
TTGCCGAAAAGCTTAACGATGTCAGGGAGGTTGATGTTGTTGTTGGTGTGGCAACGGCAGGCATTATCTGGGCTACGGGG
CTCGCGCTCCTCTCCGAGAAACCTTTAGCATATGTCCGACCCAAGAGGAAGGAGCATGGCCTACAGAGGGTTGTGGAGGG
GATTGTTGAAGGGCGTCGTGTACTAGTCGTAGACGATGTTGCTACCACGGGATCTAGCCTCGCATCGGCCGTGGAGTCTC
TCAGGGAGGCTGGTGCTGAGCCAGTAGCAGCCATGGTTATTGTAGACCGTGAGCAGGGTGCTGTTGAGAGACTCGCAGAT
TACGGTGTTAAGCTCTACAGTCTCGCAACTCTACGTGAGATAATCCATGCAATGGTTGTCAAAGGATATCTTGACCCAGC
TGAGGCCACGAGGCTTATAAACCTTCTATACGGTGAAAGCTTGTAG

Upstream 100 bases:

>100_bases
AGATAGTTGGGAGACTAATTACGTCCTCGCCGGATCCTCTCGAAGTTGCTAAGAAGGTTCTGGCGGAGCAGAAAAAGAGA
CTTGAGAGGTGCTGGAAGCC

Downstream 100 bases:

>100_bases
AGGGCTTTTGGGCTCTGGCTCGGGCTGGAAGGGCCGGGATGTAATTAGCATCCTAGATTTTGACCGGGATAGCCTTGAGC
AGCTCTTCGAGGTCGCTGAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNSYALDLADVLVEAGAIRFGEFRLSSGSTSPIYIDMRIVPSKPQLFRRVLGMLAEKLNDVREVDVVVGVATAGIIWATG
LALLSEKPLAYVRPKRKEHGLQRVVEGIVEGRRVLVVDDVATTGSSLASAVESLREAGAEPVAAMVIVDREQGAVERLAD
YGVKLYSLATLREIIHAMVVKGYLDPAEATRLINLLYGESL

Sequences:

>Translated_201_residues
MNSYALDLADVLVEAGAIRFGEFRLSSGSTSPIYIDMRIVPSKPQLFRRVLGMLAEKLNDVREVDVVVGVATAGIIWATG
LALLSEKPLAYVRPKRKEHGLQRVVEGIVEGRRVLVVDDVATTGSSLASAVESLREAGAEPVAAMVIVDREQGAVERLAD
YGVKLYSLATLREIIHAMVVKGYLDPAEATRLINLLYGESL
>Mature_201_residues
MNSYALDLADVLVEAGAIRFGEFRLSSGSTSPIYIDMRIVPSKPQLFRRVLGMLAEKLNDVREVDVVVGVATAGIIWATG
LALLSEKPLAYVRPKRKEHGLQRVVEGIVEGRRVLVVDDVATTGSSLASAVESLREAGAEPVAAMVIVDREQGAVERLAD
YGVKLYSLATLREIIHAMVVKGYLDPAEATRLINLLYGESL

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=176, Percent_Identity=34.0909090909091, Blast_Score=111, Evalue=5e-25,
Organism=Escherichia coli, GI1790073, Length=192, Percent_Identity=30.2083333333333, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508631, Length=179, Percent_Identity=31.8435754189944, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17554964, Length=175, Percent_Identity=32, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323530, Length=223, Percent_Identity=24.2152466367713, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323927, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17933654, Length=182, Percent_Identity=34.0659340659341, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_HYPBU (A2BJ25)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012331.1
- ProteinModelPortal:   A2BJ25
- SMR:   A2BJ25
- STRING:   A2BJ25
- GeneID:   4781401
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0111
- eggNOG:   arNOG09150
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- PhylomeDB:   A2BJ25
- BioCyc:   HBUT415426:HBUT_0111-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 21777; Mature: 21777

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 93-93 BINDING 97-97 BINDING 99-99 BINDING 122-122 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYALDLADVLVEAGAIRFGEFRLSSGSTSPIYIDMRIVPSKPQLFRRVLGMLAEKLND
CCCHHHHHHHHHHHHCCEEECEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
VREVDVVVGVATAGIIWATGLALLSEKPLAYVRPKRKEHGLQRVVEGIVEGRRVLVVDDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECC
ATTGSSLASAVESLREAGAEPVAAMVIVDREQGAVERLADYGVKLYSLATLREIIHAMVV
HHCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
KGYLDPAEATRLINLLYGESL
HHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSYALDLADVLVEAGAIRFGEFRLSSGSTSPIYIDMRIVPSKPQLFRRVLGMLAEKLND
CCCHHHHHHHHHHHHCCEEECEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
VREVDVVVGVATAGIIWATGLALLSEKPLAYVRPKRKEHGLQRVVEGIVEGRRVLVVDDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECC
ATTGSSLASAVESLREAGAEPVAAMVIVDREQGAVERLADYGVKLYSLATLREIIHAMVV
HHCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
KGYLDPAEATRLINLLYGESL
HHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA