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The map label for this gene is pyrB

Identifier: 124027010

GI number: 124027010

Start: 98516

End: 99463

Strand: Reverse

Name: pyrB

Synonym: Hbut_0110

Alternate gene names: 124027010

Gene position: 99463-98516 (Counterclockwise)

Preceding gene: 124027011

Following gene: 124027009

Centisome position: 5.97

GC content: 55.38

Gene sequence:

>948_bases
TTGGGCTCTGGCTCGGGCTGGAAGGGCCGGGATGTAATTAGCATCCTAGATTTTGACCGGGATAGCCTTGAGCAGCTCTT
CGAGGTCGCTGACAAGTTCTCCTCCCTGCTCAGGGAGAGGGGGCGCATACCACTCCTAGAAGGTTATATTGTCGCCTTAG
CCTTCTTTGAGCCCTCCACGAGGACCAGGCTGAGCTTTGAAACAGCCGCGAAGAGGCTAGGGGCAGACACTATAGGGTTT
ACGAGCGAAGAGGCTATCAGTATAGCCAAGGGAGAAACACTAGCCGATACTATACGTATGCTCGACTCCTATGCGGATAT
GATAGTGTTAAGGCACCGCTACGAGGGAGCAGCACTCTATGCTGCCGAAATAGCAGAACACCCGGTGATAAATGCTGGCG
ATGGTAAACAACATCACCCAACGCAGGCAATGCTCGACCTCTACACGGTCAGGAAGCTTTTCGGTACAATAGACGGGCTC
ACCTATGGGGTTCTCGGCGATCTCCGCTACGGGAGGGCCGCCTCGAGCTTCATATTGGCCCTGACAATCTATCGGCCTAG
AATGATTTACCTTATTTCGCCGCCTCTGTTGAGAGTGAGGCCCGAGGTACGCATGGTACTTGACGAGAGAGGGATGAGGT
ATAGGGAGGTTGAGAGCCTCGAGGAAGTTCTAGGCGAGCTAGACGTCCTCTACGTGACGCGTATCCAGCGGGAGAGGTTT
CCGGATCAACGCGAGTACGAGAAGGTCCGGGGGAGCTACCGCGTTACACTGGAGCTGCTAGAACAGCATGCTAGGAGGGA
GCTGCGTATCCTTCACCCGTTGCCAAGAGTTGATGAGATAGCGCCGGAGGTAGACGGGACCCCATACGCTGCCTACTTTG
AACAAGCCCGTAACGGTGTGCCAGTCCGTATGGCGCTCCTAGCTCTCATAGCTGGCCGGGAGGTGTAG

Upstream 100 bases:

>100_bases
CGTGAGATAATCCATGCAATGGTTGTCAAAGGATATCTTGACCCAGCTGAGGCCACGAGGCTTATAAACCTTCTATACGG
TGAAAGCTTGTAGAGGGCTT

Downstream 100 bases:

>100_bases
CCGGTTGGAGAGCGGGTCAGGCCTCCTGGTTAGGAGGATACGTGAGGGAACCGTGATAGACCATATACCTGCAGGTAGGG
CTTTGACTGTGTTAAAGATA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MGSGSGWKGRDVISILDFDRDSLEQLFEVADKFSSLLRERGRIPLLEGYIVALAFFEPSTRTRLSFETAAKRLGADTIGF
TSEEAISIAKGETLADTIRMLDSYADMIVLRHRYEGAALYAAEIAEHPVINAGDGKQHHPTQAMLDLYTVRKLFGTIDGL
TYGVLGDLRYGRAASSFILALTIYRPRMIYLISPPLLRVRPEVRMVLDERGMRYREVESLEEVLGELDVLYVTRIQRERF
PDQREYEKVRGSYRVTLELLEQHARRELRILHPLPRVDEIAPEVDGTPYAAYFEQARNGVPVRMALLALIAGREV

Sequences:

>Translated_315_residues
MGSGSGWKGRDVISILDFDRDSLEQLFEVADKFSSLLRERGRIPLLEGYIVALAFFEPSTRTRLSFETAAKRLGADTIGF
TSEEAISIAKGETLADTIRMLDSYADMIVLRHRYEGAALYAAEIAEHPVINAGDGKQHHPTQAMLDLYTVRKLFGTIDGL
TYGVLGDLRYGRAASSFILALTIYRPRMIYLISPPLLRVRPEVRMVLDERGMRYREVESLEEVLGELDVLYVTRIQRERF
PDQREYEKVRGSYRVTLELLEQHARRELRILHPLPRVDEIAPEVDGTPYAAYFEQARNGVPVRMALLALIAGREV
>Mature_314_residues
GSGSGWKGRDVISILDFDRDSLEQLFEVADKFSSLLRERGRIPLLEGYIVALAFFEPSTRTRLSFETAAKRLGADTIGFT
SEEAISIAKGETLADTIRMLDSYADMIVLRHRYEGAALYAAEIAEHPVINAGDGKQHHPTQAMLDLYTVRKLFGTIDGLT
YGVLGDLRYGRAASSFILALTIYRPRMIYLISPPLLRVRPEVRMVLDERGMRYREVESLEEVLGELDVLYVTRIQRERFP
DQREYEKVRGSYRVTLELLEQHARRELRILHPLPRVDEIAPEVDGTPYAAYFEQARNGVPVRMALLALIAGREV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=44.2622950819672, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI38788445, Length=308, Percent_Identity=26.2987012987013, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI2367364, Length=308, Percent_Identity=44.4805194805195, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1790703, Length=281, Percent_Identity=25.9786476868327, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=41.7956656346749, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=42.4920127795527, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322373, Length=325, Percent_Identity=23.6923076923077, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=42.7184466019417, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_HYPBU (A2BJ24)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012330.1
- ProteinModelPortal:   A2BJ24
- SMR:   A2BJ24
- STRING:   A2BJ24
- GeneID:   4781405
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0110
- eggNOG:   arNOG04260
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- PhylomeDB:   A2BJ24
- ProtClustDB:   PRK00856
- BioCyc:   HBUT415426:HBUT_0110-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35779; Mature: 35647

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGSGWKGRDVISILDFDRDSLEQLFEVADKFSSLLRERGRIPLLEGYIVALAFFEPST
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCC
RTRLSFETAAKRLGADTIGFTSEEAISIAKGETLADTIRMLDSYADMIVLRHRYEGAALY
CCEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AAEIAEHPVINAGDGKQHHPTQAMLDLYTVRKLFGTIDGLTYGVLGDLRYGRAASSFILA
HHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
LTIYRPRMIYLISPPLLRVRPEVRMVLDERGMRYREVESLEEVLGELDVLYVTRIQRERF
EEECCCEEEEEECCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDQREYEKVRGSYRVTLELLEQHARRELRILHPLPRVDEIAPEVDGTPYAAYFEQARNGV
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHCCCC
PVRMALLALIAGREV
CHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSGSGWKGRDVISILDFDRDSLEQLFEVADKFSSLLRERGRIPLLEGYIVALAFFEPST
CCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCC
RTRLSFETAAKRLGADTIGFTSEEAISIAKGETLADTIRMLDSYADMIVLRHRYEGAALY
CCEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AAEIAEHPVINAGDGKQHHPTQAMLDLYTVRKLFGTIDGLTYGVLGDLRYGRAASSFILA
HHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
LTIYRPRMIYLISPPLLRVRPEVRMVLDERGMRYREVESLEEVLGELDVLYVTRIQRERF
EEECCCEEEEEECCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDQREYEKVRGSYRVTLELLEQHARRELRILHPLPRVDEIAPEVDGTPYAAYFEQARNGV
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHCCCC
PVRMALLALIAGREV
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA