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The map label for this gene is speE [H]

Identifier: 124026960

GI number: 124026960

Start: 47041

End: 47919

Strand: Reverse

Name: speE [H]

Synonym: Hbut_0057

Alternate gene names: 124026960

Gene position: 47919-47041 (Counterclockwise)

Preceding gene: 124026963

Following gene: 124026955

Centisome position: 2.87

GC content: 52.1

Gene sequence:

>879_bases
GTGGAGCTGGGAATGTTTAGGCTAAACATTTACCAGCCTGGAGGCCCCATCGGTGCACTTTACCCCGTTGAGAAGATCCT
TTACCATGGCAGGTCACAGTACCAGGAGATAATGATTCTTGTTCTACGCGGCTTCGGTAAGACGCTTGTGCTTGACGGGC
TTATACAGAGTACCGAGAGCGACGAGCACATATACCACGAGACACTCGTTCACCCAGCTATGACGGTCCATCCTAACCCG
CGGCGTGTCCTCATACTGGGTGGTGGTGAGGGAGCAACACTTCGAGAGGTTCTGAAGCATAATACTGTTGAGAAGGCCGT
CATGGTTGATATTGATGGGGAGGTTGTTAGGGTTGCACGGGAGTATCTTCCAGAGTGGCACCAAGGCGCCTTTGACGACC
CAAGAGCACAAGTAGTCATAATGGATGGCTTTGAGTACATAAAGGAGGCAGCACGACGAGGAGAAGACTTCGACGTCATA
ATAATGGATCTCACTGACCCATTTGGCCCGAAGATAGCTGCTAAGCTCTATACGAAGGAAGCTATAGGCCTCGTTAAGTC
CGTGCTTAGGAGCGATGGTATACTTGTGACTCAGGCTGGCTGCGCCGCACTCTTCCCAGAAGCGTTTGAGAAGGTGTACG
GCTCTGTCAAGAGCCTATTCGCGCACGCTGAGGAATATGGTGTGTGGGTGCCATCGTTCATGTATGTTAACAGCTTCGTA
TTCGCCTCTGACAAGTACAGGCTGACAGACCTATCTATGGAGGAGGTTGACAGGAGGCTCCGGGAGAGGGGTGTAGAGAC
AAGGTTCTATAGCGGGCTACGCCACTACACCCTTATAGGGCTTGGCGGGATAAGGCTACTGGAAGGCAGAGGTTCCTAG

Upstream 100 bases:

>100_bases
TGGTCTGGCCTAAAGGCCCTCATCACTTCCGGTTGCGGTTTTGCTAGCCGAGTCTTATTAATATGGTTAGCTATTTCGGT
GGCTTACGGGTGTATGTGCT

Downstream 100 bases:

>100_bases
CCTTTACCATTTCCTCTACTTTTAACTAGCTCGTCTAGCTCCTGAACCTCCCTACACAGCTTCGCTACAGCCTCCACAAT
ATGCTCAGGTACCTCGTAGA

Product: spermidine synthase, SpeE

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MELGMFRLNIYQPGGPIGALYPVEKILYHGRSQYQEIMILVLRGFGKTLVLDGLIQSTESDEHIYHETLVHPAMTVHPNP
RRVLILGGGEGATLREVLKHNTVEKAVMVDIDGEVVRVAREYLPEWHQGAFDDPRAQVVIMDGFEYIKEAARRGEDFDVI
IMDLTDPFGPKIAAKLYTKEAIGLVKSVLRSDGILVTQAGCAALFPEAFEKVYGSVKSLFAHAEEYGVWVPSFMYVNSFV
FASDKYRLTDLSMEEVDRRLRERGVETRFYSGLRHYTLIGLGGIRLLEGRGS

Sequences:

>Translated_292_residues
MELGMFRLNIYQPGGPIGALYPVEKILYHGRSQYQEIMILVLRGFGKTLVLDGLIQSTESDEHIYHETLVHPAMTVHPNP
RRVLILGGGEGATLREVLKHNTVEKAVMVDIDGEVVRVAREYLPEWHQGAFDDPRAQVVIMDGFEYIKEAARRGEDFDVI
IMDLTDPFGPKIAAKLYTKEAIGLVKSVLRSDGILVTQAGCAALFPEAFEKVYGSVKSLFAHAEEYGVWVPSFMYVNSFV
FASDKYRLTDLSMEEVDRRLRERGVETRFYSGLRHYTLIGLGGIRLLEGRGS
>Mature_292_residues
MELGMFRLNIYQPGGPIGALYPVEKILYHGRSQYQEIMILVLRGFGKTLVLDGLIQSTESDEHIYHETLVHPAMTVHPNP
RRVLILGGGEGATLREVLKHNTVEKAVMVDIDGEVVRVAREYLPEWHQGAFDDPRAQVVIMDGFEYIKEAARRGEDFDVI
IMDLTDPFGPKIAAKLYTKEAIGLVKSVLRSDGILVTQAGCAALFPEAFEKVYGSVKSLFAHAEEYGVWVPSFMYVNSFV
FASDKYRLTDLSMEEVDRRLRERGVETRFYSGLRHYTLIGLGGIRLLEGRGS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=198, Percent_Identity=40.4040404040404, Blast_Score=158, Evalue=5e-39,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=35.7142857142857, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=40.0881057268723, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25154106, Length=192, Percent_Identity=42.7083333333333, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6325326, Length=211, Percent_Identity=39.8104265402844, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323175, Length=214, Percent_Identity=39.2523364485981, Blast_Score=162, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24645443, Length=264, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21355941, Length=213, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24663238, Length=226, Percent_Identity=30.5309734513274, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358269, Length=226, Percent_Identity=30.5309734513274, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32856; Mature: 32856

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELGMFRLNIYQPGGPIGALYPVEKILYHGRSQYQEIMILVLRGFGKTLVLDGLIQSTES
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DEHIYHETLVHPAMTVHPNPRRVLILGGGEGATLREVLKHNTVEKAVMVDIDGEVVRVAR
CHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHH
EYLPEWHQGAFDDPRAQVVIMDGFEYIKEAARRGEDFDVIIMDLTDPFGPKIAAKLYTKE
HHCCHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHH
AIGLVKSVLRSDGILVTQAGCAALFPEAFEKVYGSVKSLFAHAEEYGVWVPSFMYVNSFV
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEE
FASDKYRLTDLSMEEVDRRLRERGVETRFYSGLRHYTLIGLGGIRLLEGRGS
EECCCEEEECCCHHHHHHHHHHCCCHHHHHHCCHHEEEEECCCEEEECCCCC
>Mature Secondary Structure
MELGMFRLNIYQPGGPIGALYPVEKILYHGRSQYQEIMILVLRGFGKTLVLDGLIQSTES
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DEHIYHETLVHPAMTVHPNPRRVLILGGGEGATLREVLKHNTVEKAVMVDIDGEVVRVAR
CHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHH
EYLPEWHQGAFDDPRAQVVIMDGFEYIKEAARRGEDFDVIIMDLTDPFGPKIAAKLYTKE
HHCCHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHH
AIGLVKSVLRSDGILVTQAGCAALFPEAFEKVYGSVKSLFAHAEEYGVWVPSFMYVNSFV
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEE
FASDKYRLTDLSMEEVDRRLRERGVETRFYSGLRHYTLIGLGGIRLLEGRGS
EECCCEEEECCCHHHHHHHHHHCCCHHHHHHCCHHEEEEECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA