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The map label for this gene is speB [C]

Identifier: 123967110

GI number: 123967110

Start: 1665399

End: 1666283

Strand: Direct

Name: speB [C]

Synonym: P9515_18771

Alternate gene names: 123967110

Gene position: 1665399-1666283 (Clockwise)

Preceding gene: 123967109

Following gene: 123967111

Centisome position: 97.72

GC content: 32.88

Gene sequence:

>885_bases
ATGATAAATAAAAATTTGTTTGATAAAGAAAAAGCAATTTTTATGGGGTCAAAAAGAAATCCTGATAATTGTTCAATTGG
AATATTTGGAGTCAATTATGATGGGACATGCTCCTATAAGTCAGGAGCTAAATTTGGTCCTAATGCCATAAGGCAAGTTA
GTACATGCTTAGAAACATTTTGTCCAAGATTAGAAAAAGATTTAGAAGATTTTAATTATGTTGATTTTGGCTCGTTAATA
ATTCATAAAAAGGACTCAAAGTCTGTCATTAAATCAGTCAAATCAGCAACAAATTTTTTAATGAGTAAAAGCCTAGCTCC
CATTATGCTTGGAGGTGAACACTCTATTACGAGGGGTGCTATTGAGGCATTAGTCAAAAAATATCCAGATTTGATTTTAA
TTCAACTAGATGCTCATGCAGATTTAAGAAGTTCATATATGGGAAACGAACATAGTCATGCTTGTACAATGCAAAGATGC
TTAGATGTCTTACCTGAAAAAAAGATTTTGCAAGTAGGGATAAGGAGTGGAACAAAAGAAGAGTTTAAATTTATGAGACA
TCAAAATCAATTAGTAAGATTCTCCCCAGGCGGGAATACCCAAGAATTAAAAAAGGCTCTTATTCCTTACTCTAATTCTC
CAATATATTTAACAATAGATTTAGATTGGTTTGATCCAAGTTTATTACCTGGCACAGGAACTCCCGAACCAGGAGGATTT
TTTTGGAACGACTTTGAAGCGATTCTCGACACTTTAAAAAACATTAGAATAGTTGCTTCAGATATTGTGGAATTATCTCC
AGATATTGATAACAGCGGAGTAAGTAGTGTCGTTGCCGCAAAAGTACTAAGGAGCTTAATTATGTCATTAAAAAATATGC
AATAA

Upstream 100 bases:

>100_bases
ACAACAATAGAAAAGGGATGTGATATTTGGAATTTAAATTTTCAAAATGCGGCATTCAAAATGATGCCAAATAAAATTGT
GAAAGAACTAAATTCGTAAA

Downstream 100 bases:

>100_bases
AAAAATTCTAAACTAAAGTATAGGAAACTAAAAAAATCGTTTAATATTTCATGGATTTACTTAAAAGTCCACTTTATTCA
AAATATATTGAGTCAAATGC

Product: arginase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MINKNLFDKEKAIFMGSKRNPDNCSIGIFGVNYDGTCSYKSGAKFGPNAIRQVSTCLETFCPRLEKDLEDFNYVDFGSLI
IHKKDSKSVIKSVKSATNFLMSKSLAPIMLGGEHSITRGAIEALVKKYPDLILIQLDAHADLRSSYMGNEHSHACTMQRC
LDVLPEKKILQVGIRSGTKEEFKFMRHQNQLVRFSPGGNTQELKKALIPYSNSPIYLTIDLDWFDPSLLPGTGTPEPGGF
FWNDFEAILDTLKNIRIVASDIVELSPDIDNSGVSSVVAAKVLRSLIMSLKNMQ

Sequences:

>Translated_294_residues
MINKNLFDKEKAIFMGSKRNPDNCSIGIFGVNYDGTCSYKSGAKFGPNAIRQVSTCLETFCPRLEKDLEDFNYVDFGSLI
IHKKDSKSVIKSVKSATNFLMSKSLAPIMLGGEHSITRGAIEALVKKYPDLILIQLDAHADLRSSYMGNEHSHACTMQRC
LDVLPEKKILQVGIRSGTKEEFKFMRHQNQLVRFSPGGNTQELKKALIPYSNSPIYLTIDLDWFDPSLLPGTGTPEPGGF
FWNDFEAILDTLKNIRIVASDIVELSPDIDNSGVSSVVAAKVLRSLIMSLKNMQ
>Mature_294_residues
MINKNLFDKEKAIFMGSKRNPDNCSIGIFGVNYDGTCSYKSGAKFGPNAIRQVSTCLETFCPRLEKDLEDFNYVDFGSLI
IHKKDSKSVIKSVKSATNFLMSKSLAPIMLGGEHSITRGAIEALVKKYPDLILIQLDAHADLRSSYMGNEHSHACTMQRC
LDVLPEKKILQVGIRSGTKEEFKFMRHQNQLVRFSPGGNTQELKKALIPYSNSPIYLTIDLDWFDPSLLPGTGTPEPGGF
FWNDFEAILDTLKNIRIVASDIVELSPDIDNSGVSSVVAAKVLRSLIMSLKNMQ

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=289, Percent_Identity=28.0276816608997, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502215, Length=299, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789306, Length=251, Percent_Identity=28.2868525896414, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325146, Length=204, Percent_Identity=30.3921568627451, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32705; Mature: 32705

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKNLFDKEKAIFMGSKRNPDNCSIGIFGVNYDGTCSYKSGAKFGPNAIRQVSTCLETF
CCCCCCCCCHHHEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CPRLEKDLEDFNYVDFGSLIIHKKDSKSVIKSVKSATNFLMSKSLAPIMLGGEHSITRGA
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
IEALVKKYPDLILIQLDAHADLRSSYMGNEHSHACTMQRCLDVLPEKKILQVGIRSGTKE
HHHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHH
EFKFMRHQNQLVRFSPGGNTQELKKALIPYSNSPIYLTIDLDWFDPSLLPGTGTPEPGGF
HHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
FWNDFEAILDTLKNIRIVASDIVELSPDIDNSGVSSVVAAKVLRSLIMSLKNMQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINKNLFDKEKAIFMGSKRNPDNCSIGIFGVNYDGTCSYKSGAKFGPNAIRQVSTCLETF
CCCCCCCCCHHHEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CPRLEKDLEDFNYVDFGSLIIHKKDSKSVIKSVKSATNFLMSKSLAPIMLGGEHSITRGA
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
IEALVKKYPDLILIQLDAHADLRSSYMGNEHSHACTMQRCLDVLPEKKILQVGIRSGTKE
HHHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHH
EFKFMRHQNQLVRFSPGGNTQELKKALIPYSNSPIYLTIDLDWFDPSLLPGTGTPEPGGF
HHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
FWNDFEAILDTLKNIRIVASDIVELSPDIDNSGVSSVVAAKVLRSLIMSLKNMQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]