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The map label for this gene is speE

Identifier: 123967109

GI number: 123967109

Start: 1664546

End: 1665397

Strand: Direct

Name: speE

Synonym: P9515_18761

Alternate gene names: 123967109

Gene position: 1664546-1665397 (Clockwise)

Preceding gene: 123967108

Following gene: 123967110

Centisome position: 97.67

GC content: 30.4

Gene sequence:

>852_bases
ATGAAAGATATAACAACGTGGATTGATGAATACCAAAAAGGTTCAAGATTTGGCCTTAAAGGAAAAGTTTTACTCAAAAA
AAATTCTAAATTTCAAGAAATCCTGATCATAGAAAGTGATTATTATGGTAAGGCTTTAATGTTAGATAGATGTTGGATGA
CATCAATGAGAGACGAAAAATATTATCATGAATGTTTGGTTCATCCAGCCTTAAGTAGCATAAAAGAGAAATCTCGCATA
TTAATAATTGGAGGGGGTGATGGTGGAACCGCAAGAGAATGCTTAAAATATTCTCAAGTAGAAAAAATAGATTTGGTAGA
AATTGATGAGGAAGTCATAAAGGCATCTAAAAAATTTTTGCAAGAAATTGGAGGTACTGCCTGGATTGATAAAAGATTAA
CCATACATATTGATGATGGTGTTAAGTGGGTAGAAAAAGCAAAAAATAATTTTTATGATTGCATTTTTATAGATTGTTCA
GATCCCTCAGAATTTTCTAACTTATTATTTACAGATACTTTTTATAAAGAATGCAAAAGAATACTTACGCAAAAGGGAAT
ATTAGCTACTCAAAGCGAATCACCTGAATCATTCAAAAATATACATATACATATTTTGAAATCTCTTAAGAAAGTATTTA
AATTATCTGAAACTATGTACTCATTTGTGCCCATATACCCAAGTGGTATTTGGAGCTGGACATTCGCCTCAAAAGGAGAA
TTGAATTTATCAACACCATGTTGCAATGAGGTTACAACAATAGAAAAGGGATGTGATATTTGGAATTTAAATTTTCAAAA
TGCGGCATTCAAAATGATGCCAAATAAAATTGTGAAAGAACTAAATTCGTAA

Upstream 100 bases:

>100_bases
ATCTTGCAGGAGATAATATTAAAAAACAAACTCTAAAAGAATTTAAGCGACTTTGGAAAGTTGCTAAAAAAACATTATTT
ATAGAAAATCTAAAAAACAA

Downstream 100 bases:

>100_bases
AATGATAAATAAAAATTTGTTTGATAAAGAAAAAGCAATTTTTATGGGGTCAAAAAGAAATCCTGATAATTGTTCAATTG
GAATATTTGGAGTCAATTAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKDITTWIDEYQKGSRFGLKGKVLLKKNSKFQEILIIESDYYGKALMLDRCWMTSMRDEKYYHECLVHPALSSIKEKSRI
LIIGGGDGGTARECLKYSQVEKIDLVEIDEEVIKASKKFLQEIGGTAWIDKRLTIHIDDGVKWVEKAKNNFYDCIFIDCS
DPSEFSNLLFTDTFYKECKRILTQKGILATQSESPESFKNIHIHILKSLKKVFKLSETMYSFVPIYPSGIWSWTFASKGE
LNLSTPCCNEVTTIEKGCDIWNLNFQNAAFKMMPNKIVKELNS

Sequences:

>Translated_283_residues
MKDITTWIDEYQKGSRFGLKGKVLLKKNSKFQEILIIESDYYGKALMLDRCWMTSMRDEKYYHECLVHPALSSIKEKSRI
LIIGGGDGGTARECLKYSQVEKIDLVEIDEEVIKASKKFLQEIGGTAWIDKRLTIHIDDGVKWVEKAKNNFYDCIFIDCS
DPSEFSNLLFTDTFYKECKRILTQKGILATQSESPESFKNIHIHILKSLKKVFKLSETMYSFVPIYPSGIWSWTFASKGE
LNLSTPCCNEVTTIEKGCDIWNLNFQNAAFKMMPNKIVKELNS
>Mature_283_residues
MKDITTWIDEYQKGSRFGLKGKVLLKKNSKFQEILIIESDYYGKALMLDRCWMTSMRDEKYYHECLVHPALSSIKEKSRI
LIIGGGDGGTARECLKYSQVEKIDLVEIDEEVIKASKKFLQEIGGTAWIDKRLTIHIDDGVKWVEKAKNNFYDCIFIDCS
DPSEFSNLLFTDTFYKECKRILTQKGILATQSESPESFKNIHIHILKSLKKVFKLSETMYSFVPIYPSGIWSWTFASKGE
LNLSTPCCNEVTTIEKGCDIWNLNFQNAAFKMMPNKIVKELNS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=268, Percent_Identity=31.7164179104478, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1786312, Length=276, Percent_Identity=31.1594202898551, Blast_Score=130, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI212645616, Length=230, Percent_Identity=35.6521739130435, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25154106, Length=257, Percent_Identity=35.0194552529183, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325326, Length=269, Percent_Identity=36.0594795539033, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6323175, Length=212, Percent_Identity=36.7924528301887, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645443, Length=236, Percent_Identity=33.8983050847458, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355941, Length=159, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_PROM5 (A2BZ72)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012190.1
- ProteinModelPortal:   A2BZ72
- SMR:   A2BZ72
- STRING:   A2BZ72
- GeneID:   4720526
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18761
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   WSWTFAS
- ProtClustDB:   PRK00811
- BioCyc:   PMAR167542:P9515ORF_1959-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32615; Mature: 32615

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 32-32 BINDING 87-87 BINDING 107-107 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDITTWIDEYQKGSRFGLKGKVLLKKNSKFQEILIIESDYYGKALMLDRCWMTSMRDEK
CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHH
YYHECLVHPALSSIKEKSRILIIGGGDGGTARECLKYSQVEKIDLVEIDEEVIKASKKFL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QEIGGTAWIDKRLTIHIDDGVKWVEKAKNNFYDCIFIDCSDPSEFSNLLFTDTFYKECKR
HHCCCCEEECCEEEEEECCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
ILTQKGILATQSESPESFKNIHIHILKSLKKVFKLSETMYSFVPIYPSGIWSWTFASKGE
HHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
LNLSTPCCNEVTTIEKGCDIWNLNFQNAAFKMMPNKIVKELNS
CCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKDITTWIDEYQKGSRFGLKGKVLLKKNSKFQEILIIESDYYGKALMLDRCWMTSMRDEK
CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHH
YYHECLVHPALSSIKEKSRILIIGGGDGGTARECLKYSQVEKIDLVEIDEEVIKASKKFL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QEIGGTAWIDKRLTIHIDDGVKWVEKAKNNFYDCIFIDCSDPSEFSNLLFTDTFYKECKR
HHCCCCEEECCEEEEEECCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
ILTQKGILATQSESPESFKNIHIHILKSLKKVFKLSETMYSFVPIYPSGIWSWTFASKGE
HHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
LNLSTPCCNEVTTIEKGCDIWNLNFQNAAFKMMPNKIVKELNS
CCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA