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The map label for this gene is gidA

Identifier: 123967098

GI number: 123967098

Start: 1653859

End: 1655823

Strand: Direct

Name: gidA

Synonym: P9515_18651

Alternate gene names: 123967098

Gene position: 1653859-1655823 (Clockwise)

Preceding gene: 123967097

Following gene: 123967099

Centisome position: 97.05

GC content: 33.84

Gene sequence:

>1965_bases
ATGAAAAAGCAACAATCCTCCAATGAATCTTTTGACGTTATTGTTATTGGAGGAGGACATGCAGGATGTGAAGCTGCCAT
TACGACAGCTAAATTAGGCTTTTCAACCGCATTATTCACAATAAATTTGGATAGAATTGCTTGGCAACCATGCAACCCCG
CGGTAGGTGGACCAGCAAAAAGTCAATTAGTACATGAAGTTGATGCTTTAGGTGGAATCATTGGGAAACTAGCTGATGAG
ACAGCAATACAGAAAAGAATATTAAATGCGAGCAGAGGGCCAGCAGTTTGGGCATTAAGGGCTCAAACCGATAAAAGAGA
ATATTCGAAACGAATGATAGAGATACTACAAAACACTGATAATCTATCTTTGAAAGAAGCCATGATTACTGAATTATTGA
TAAAAGAGGTTGAAACTTTTACCAAAAACTCAAAGAATACGACAAAGCAAATTAAAGGTGTGAAAACTTTTTTTGGAACC
TGCTATTCTGCAAAATCAATAATAATAACTGCCGGAACTTTTTTGGAAGGGAGAATCTGGATAGGTAATAAATCGATGTC
TGCAGGGAGATCCGGAGAGCAAGCGGCTCAAGGTTTAACTGAAAGCTTACATAGTCTTGGAATAAAAACTGAGAGACTAA
AAACTGGAACTCCTGCAAGAGTGGATAAAAAAAGTATTTCTTTTGATGCATTAGATATTCAGCCAAGCACAGCTTCAGAT
AAATATTTTTCATTTGATCCCAAAATAAAAAACGACATGCCACAAATTTGTTGCCACATAACACGAACAACTCAAAAAAC
TCATGAATTAATTCGTAATAATTTGCATTTAACCCCAATTTATGGAGGATTTATAGATAGTAAAGGACCTCGATATTGCC
CTTCTATAGAAGACAAAATCGTAAAATTTGCAGATAAAAATTCACATCAAATTTTCTTAGAACCTGAAGGGATAAATACG
CCTGAAATTTACGTACAAGGATTCTCAACTGGGTTACCTGAAAATATTCAATTAGAACTTTTAAGAACATTACCAGGTTT
AAATAAATGTAAAATGCTTAGGCCTGCTTATGCTGTCGAGTATGAATACATTCCTGCAACTCAACTTAAGTTATCATTGG
AAACTATTGAGATAGACAATTTATTTAGTGCTGGACAAATCAATGGTACAACTGGCTATGAAGAGGCTGCTGCTCAAGGA
TTAGTAGCTGGAATAAATGCAACTAGAAAACTTAATATGAAAGATCCAATAATCTTTTCAAGAGAGAGCAGCTATATAGG
CACTATGATCAATGACTTAATAACACGAGATCTAAAAGAACCATATAGAGTTCTCACAAGTAGAAGTGAATACAGACTTA
CTTTACGAGGAGACAATGCCGATAGGAGATTGACTCCTTTGGGTTTTGAAATAGGATTAATTGATGAACGAAGATGGTTT
GCTCATAAAGAAAAAATGAAATTACTTAAAGAAGAAAATTCAAGATTAGAAAATACACGTTTAAAATGTACTGATGAAAT
CGCCAGAAACATAGAGTTAGAAAGTGGCTCAAAAATTAAAGGATCGACGACATTGAAAGATCTTTTAAAAAGACCAAATG
TTCACTACTCTGATTTTATAAAATATGATTTGGCAAACAAAAGTCTACCAATAGCAGTTATGGAAGGAGTTGAAATAGAC
ATTAAATACGAAGGTTACCTTAAAAGACAACAAAATAATATCGATCAGATAAATCGTCAAAGTCTCAAATCACTGCCAAT
CGAAATTAATTATGACCTAATAGATACTCTATCTTTAGAGGCCCGCGAAAACTTAAATAAAATAAAACCTACTAATTTTG
GGGATGCCTCCAAAATACCCGGTGTCAGTAAAGCTGATTTAACAGCATTACTAGTTTGGCTTAAAATAAAAGAAATAAAA
AAAGAAAAGACAAATAGTTTTGTTGAAAAAAAGTTATCATCTTAA

Upstream 100 bases:

>100_bases
TGTCACTAAACATAGAAATGGACCCGTTGGAACTGTTAAATTATTATTTGAACCACAATTTACAAGATTTAGGAATTTAG
CTAATTAATTCATCACGAAA

Downstream 100 bases:

>100_bases
AAGAAATTGGTATCATCACTGAATAATAAACTCTTTAACTCACCAAGAATTTTATGGGAAGAGAAAGCTACTGCTTTCTT
AGTTGAAAATTCTTTACCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MKKQQSSNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKRMIEILQNTDNLSLKEAMITELLIKEVETFTKNSKNTTKQIKGVKTFFGT
CYSAKSIIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTESLHSLGIKTERLKTGTPARVDKKSISFDALDIQPSTASD
KYFSFDPKIKNDMPQICCHITRTTQKTHELIRNNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFADKNSHQIFLEPEGINT
PEIYVQGFSTGLPENIQLELLRTLPGLNKCKMLRPAYAVEYEYIPATQLKLSLETIEIDNLFSAGQINGTTGYEEAAAQG
LVAGINATRKLNMKDPIIFSRESSYIGTMINDLITRDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGFEIGLIDERRWF
AHKEKMKLLKEENSRLENTRLKCTDEIARNIELESGSKIKGSTTLKDLLKRPNVHYSDFIKYDLANKSLPIAVMEGVEID
IKYEGYLKRQQNNIDQINRQSLKSLPIEINYDLIDTLSLEARENLNKIKPTNFGDASKIPGVSKADLTALLVWLKIKEIK
KEKTNSFVEKKLSS

Sequences:

>Translated_654_residues
MKKQQSSNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKRMIEILQNTDNLSLKEAMITELLIKEVETFTKNSKNTTKQIKGVKTFFGT
CYSAKSIIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTESLHSLGIKTERLKTGTPARVDKKSISFDALDIQPSTASD
KYFSFDPKIKNDMPQICCHITRTTQKTHELIRNNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFADKNSHQIFLEPEGINT
PEIYVQGFSTGLPENIQLELLRTLPGLNKCKMLRPAYAVEYEYIPATQLKLSLETIEIDNLFSAGQINGTTGYEEAAAQG
LVAGINATRKLNMKDPIIFSRESSYIGTMINDLITRDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGFEIGLIDERRWF
AHKEKMKLLKEENSRLENTRLKCTDEIARNIELESGSKIKGSTTLKDLLKRPNVHYSDFIKYDLANKSLPIAVMEGVEID
IKYEGYLKRQQNNIDQINRQSLKSLPIEINYDLIDTLSLEARENLNKIKPTNFGDASKIPGVSKADLTALLVWLKIKEIK
KEKTNSFVEKKLSS
>Mature_654_residues
MKKQQSSNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKRMIEILQNTDNLSLKEAMITELLIKEVETFTKNSKNTTKQIKGVKTFFGT
CYSAKSIIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTESLHSLGIKTERLKTGTPARVDKKSISFDALDIQPSTASD
KYFSFDPKIKNDMPQICCHITRTTQKTHELIRNNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFADKNSHQIFLEPEGINT
PEIYVQGFSTGLPENIQLELLRTLPGLNKCKMLRPAYAVEYEYIPATQLKLSLETIEIDNLFSAGQINGTTGYEEAAAQG
LVAGINATRKLNMKDPIIFSRESSYIGTMINDLITRDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGFEIGLIDERRWF
AHKEKMKLLKEENSRLENTRLKCTDEIARNIELESGSKIKGSTTLKDLLKRPNVHYSDFIKYDLANKSLPIAVMEGVEID
IKYEGYLKRQQNNIDQINRQSLKSLPIEINYDLIDTLSLEARENLNKIKPTNFGDASKIPGVSKADLTALLVWLKIKEIK
KEKTNSFVEKKLSS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=657, Percent_Identity=38.2039573820396, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI19882217, Length=682, Percent_Identity=36.8035190615836, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI183227703, Length=697, Percent_Identity=36.0114777618364, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI2367273, Length=628, Percent_Identity=43.6305732484076, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17534255, Length=630, Percent_Identity=36.8253968253968, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321202, Length=631, Percent_Identity=40.8874801901743, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24658174, Length=645, Percent_Identity=37.6744186046512, Blast_Score=384, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_PROM5 (A2BZ61)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012179.1
- ProteinModelPortal:   A2BZ61
- SMR:   A2BZ61
- STRING:   A2BZ61
- GeneID:   4720443
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18651
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   PMAR167542:P9515ORF_1947-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 73177; Mature: 73177

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQQSSNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAK
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEHHEEECCCCCCCCCCHH
SQLVHEVDALGGIIGKLADETAIQKRILNASRGPAVWALRAQTDKREYSKRMIEILQNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCC
NLSLKEAMITELLIKEVETFTKNSKNTTKQIKGVKTFFGTCYSAKSIIITAGTFLEGRIW
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEE
IGNKSMSAGRSGEQAAQGLTESLHSLGIKTERLKTGTPARVDKKSISFDALDIQPSTASD
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
KYFSFDPKIKNDMPQICCHITRTTQKTHELIRNNLHLTPIYGGFIDSKGPRYCPSIEDKI
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHH
VKFADKNSHQIFLEPEGINTPEIYVQGFSTGLPENIQLELLRTLPGLNKCKMLRPAYAVE
HHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCHHEEE
YEYIPATQLKLSLETIEIDNLFSAGQINGTTGYEEAAAQGLVAGINATRKLNMKDPIIFS
EEECCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHCCCCCCEEECCCCCEEEE
RESSYIGTMINDLITRDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGFEIGLIDERRWF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEEEECCHHHH
AHKEKMKLLKEENSRLENTRLKCTDEIARNIELESGSKIKGSTTLKDLLKRPNVHYSDFI
HHHHHHHHHHHHCCHHHHCCCEEHHHHHHCEECCCCCCCCCCHHHHHHHHCCCCCHHHHH
KYDLANKSLPIAVMEGVEIDIKYEGYLKRQQNNIDQINRQSLKSLPIEINYDLIDTLSLE
EEECCCCCCCEEEECCEEEEEEECCHHHHHHCCHHHHHHHHHHCCCEEEECCHHHHCCHH
ARENLNKIKPTNFGDASKIPGVSKADLTALLVWLKIKEIKKEKTNSFVEKKLSS
HHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKQQSSNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAK
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEHHEEECCCCCCCCCCHH
SQLVHEVDALGGIIGKLADETAIQKRILNASRGPAVWALRAQTDKREYSKRMIEILQNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCC
NLSLKEAMITELLIKEVETFTKNSKNTTKQIKGVKTFFGTCYSAKSIIITAGTFLEGRIW
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEE
IGNKSMSAGRSGEQAAQGLTESLHSLGIKTERLKTGTPARVDKKSISFDALDIQPSTASD
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
KYFSFDPKIKNDMPQICCHITRTTQKTHELIRNNLHLTPIYGGFIDSKGPRYCPSIEDKI
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHH
VKFADKNSHQIFLEPEGINTPEIYVQGFSTGLPENIQLELLRTLPGLNKCKMLRPAYAVE
HHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCHHEEE
YEYIPATQLKLSLETIEIDNLFSAGQINGTTGYEEAAAQGLVAGINATRKLNMKDPIIFS
EEECCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHCCCCCCEEECCCCCEEEE
RESSYIGTMINDLITRDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGFEIGLIDERRWF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEEEECCHHHH
AHKEKMKLLKEENSRLENTRLKCTDEIARNIELESGSKIKGSTTLKDLLKRPNVHYSDFI
HHHHHHHHHHHHCCHHHHCCCEEHHHHHHCEECCCCCCCCCCHHHHHHHHCCCCCHHHHH
KYDLANKSLPIAVMEGVEIDIKYEGYLKRQQNNIDQINRQSLKSLPIEINYDLIDTLSLE
EEECCCCCCCEEEECCEEEEEEECCHHHHHHCCHHHHHHHHHHCCCEEEECCHHHHCCHH
ARENLNKIKPTNFGDASKIPGVSKADLTALLVWLKIKEIKKEKTNSFVEKKLSS
HHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA