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The map label for this gene is dnaB [H]

Identifier: 123967097

GI number: 123967097

Start: 1652464

End: 1653846

Strand: Direct

Name: dnaB [H]

Synonym: P9515_18641

Alternate gene names: 123967097

Gene position: 1652464-1653846 (Clockwise)

Preceding gene: 123967096

Following gene: 123967098

Centisome position: 96.97

GC content: 35.14

Gene sequence:

>1383_bases
ATGGTTTCGGTTCCTTTCCCAAATAATGGGTCTAATAAAAATTTCAAAAAAGACTATAGTAGCGAAAATGCAGGATTAGT
TCCTCCCCAAAATATTCAAGCAGAAGAAGCTATTTTAGGTGGTATTCTTCTTGATCCGGATGCAATTGGAAGAATTGCAG
ATTTAATTAAACCAGAAGCTTTTTATATAAATGCGCATCAAGATATTTTCAAAACAGCCCTAATGTTACACACGCAAGGC
AAACCTACAGATTTAACATCAATGAGTGCGTGGCTTGCAGACAATGGATCACTTGAAAAAATTGGAGGAAATTCTAAATT
AGTAGAACTAGTAGAAAACGTTTCTTCCACAGCTTCAATAGAACAAGTAGCGAATTTAATAAGTGATAAATTTATACGGA
GACAACTAATCCGATCTGGGAATGAGGTAATTCAGCTAGGATTTGATCAAACACAAGAAACTAATGAAGTATTAGATAAG
GCAGAGCAAAAAATTTTTGAAATTAGCCAAGAAAAACCCACTAAAGGTCTTACTCAGGCGGCAGAAATTCTTACAAGTAC
TTTTAATGAAATAGAGTCAAGGTCGTTGGGTAAATCTGTTCCTGGTATTCCGGTCAATTTTTATGATCTTGATGCAATGA
CTCAAGGTTTTCAAAGAAGTGATTTAATAATTGTGGCAGGAAGACCATCGATGGGTAAAACTTCCATGGTTTTGAACCTA
GCAAAGAATGTAGCTCAATCTCAGGATCTTCCTGTTTGTGTCTTCAGCCTTGAGATGAGTAAAGAGCAACTTACTTATAG
ATTACTTTCTATGGAAGTGGGTATAGAAAGTGGAAGATTAAGAACAGGTAGACTACAACAAGATGAATGGCCTTTACTTG
GAGAAGGAATCAACTCATTAGGTCAACTTCCCATTTTTATTGATGATAAACCTAATTTAAGTGTTTTAGAGATGAGATCG
CTATGCCGAAGATTAATAGCTGAGCAGAAAAAAGAACTGGGTTTAATAGTTATTGACTACCTGCAACTAATGGAAGGTAC
AACACCAGACAACAGAGTTCAAGAGCTATCTCGTATTACTAGAGGTCTTAAAAGCATGGCAAGAGAGTTAAAAGTACCAG
TAGTAGCCTTATCTCAGTTAAGTAGAGGAGTTGAGTCAAGAACAAATAAAAGGCCTATGTTAAGTGACCTTAGAGAATCG
GGTTCTATAGAACAAGATGCCGACTTAGTATTAATGATTTATAGAGATGAATATTACAATCCAGAAACTGAAGATAGAGG
CATAACTGAAATTATTGTCACTAAACATAGAAATGGACCCGTTGGAACTGTTAAATTATTATTTGAACCACAATTTACAA
GATTTAGGAATTTAGCTAATTAA

Upstream 100 bases:

>100_bases
CAAGAAGTAAGTGCGGAAGTTAACATTGAAGTAACAAGTTAATAACTTTGTTGCATTATTTCTAAAAGTAGTCAGAGTAT
ATATCTAAGTCTTCAATTAT

Downstream 100 bases:

>100_bases
TTCATCACGAAAATGAAAAAGCAACAATCCTCCAATGAATCTTTTGACGTTATTGTTATTGGAGGAGGACATGCAGGATG
TGAAGCTGCCATTACGACAG

Product: DnaB replicative helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MVSVPFPNNGSNKNFKKDYSSENAGLVPPQNIQAEEAILGGILLDPDAIGRIADLIKPEAFYINAHQDIFKTALMLHTQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLISDKFIRRQLIRSGNEVIQLGFDQTQETNEVLDK
AEQKIFEISQEKPTKGLTQAAEILTSTFNEIESRSLGKSVPGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSMVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGTTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPETEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN

Sequences:

>Translated_460_residues
MVSVPFPNNGSNKNFKKDYSSENAGLVPPQNIQAEEAILGGILLDPDAIGRIADLIKPEAFYINAHQDIFKTALMLHTQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLISDKFIRRQLIRSGNEVIQLGFDQTQETNEVLDK
AEQKIFEISQEKPTKGLTQAAEILTSTFNEIESRSLGKSVPGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSMVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGTTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPETEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
>Mature_460_residues
MVSVPFPNNGSNKNFKKDYSSENAGLVPPQNIQAEEAILGGILLDPDAIGRIADLIKPEAFYINAHQDIFKTALMLHTQG
KPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASIEQVANLISDKFIRRQLIRSGNEVIQLGFDQTQETNEVLDK
AEQKIFEISQEKPTKGLTQAAEILTSTFNEIESRSLGKSVPGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSMVLNL
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSLGQLPIFIDDKPNLSVLEMRS
LCRRLIAEQKKELGLIVIDYLQLMEGTTPDNRVQELSRITRGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRES
GSIEQDADLVLMIYRDEYYNPETEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=444, Percent_Identity=43.2432432432432, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51268; Mature: 51268

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVPFPNNGSNKNFKKDYSSENAGLVPPQNIQAEEAILGGILLDPDAIGRIADLIKPEA
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCE
FYINAHQDIFKTALMLHTQGKPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASI
EEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHH
EQVANLISDKFIRRQLIRSGNEVIQLGFDQTQETNEVLDKAEQKIFEISQEKPTKGLTQA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AEILTSTFNEIESRSLGKSVPGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSMVLNL
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCCCHHHHHHHH
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQLPIFIDDKPNLSVLEMRSLCRRLIAEQKKELGLIVIDYLQLMEGTTPDNRVQELSRIT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRESGSIEQDADLVLMIYRDEYYN
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCC
PETEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHCCCC
>Mature Secondary Structure
MVSVPFPNNGSNKNFKKDYSSENAGLVPPQNIQAEEAILGGILLDPDAIGRIADLIKPEA
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCE
FYINAHQDIFKTALMLHTQGKPTDLTSMSAWLADNGSLEKIGGNSKLVELVENVSSTASI
EEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHH
EQVANLISDKFIRRQLIRSGNEVIQLGFDQTQETNEVLDKAEQKIFEISQEKPTKGLTQA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AEILTSTFNEIESRSLGKSVPGIPVNFYDLDAMTQGFQRSDLIIVAGRPSMGKTSMVLNL
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCCCHHHHHHHH
AKNVAQSQDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLQQDEWPLLGEGINSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQLPIFIDDKPNLSVLEMRSLCRRLIAEQKKELGLIVIDYLQLMEGTTPDNRVQELSRIT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RGLKSMARELKVPVVALSQLSRGVESRTNKRPMLSDLRESGSIEQDADLVLMIYRDEYYN
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCC
PETEDRGITEIIVTKHRNGPVGTVKLLFEPQFTRFRNLAN
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]