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The map label for this gene is tig

Identifier: 123967078

GI number: 123967078

Start: 1632255

End: 1633676

Strand: Direct

Name: tig

Synonym: P9515_18451

Alternate gene names: 123967078

Gene position: 1632255-1633676 (Clockwise)

Preceding gene: 123967074

Following gene: 123967079

Centisome position: 95.78

GC content: 30.1

Gene sequence:

>1422_bases
ATGATTAAAGAAGCATTAATAGTAAAAACAACCGCACTGCCTCAAAGTAGAATTGCTCTTGAATTAGAAATCCCATCTAA
TACATGCAAATCATTCGTCAGTGAAACAATAAATTCAATTAGTCGTTCAGCCAAAATCCCAGGATTTAGGCTTGGCAAAA
TTCCAAAACAAGTATTAATACAAAGAATTGGTATAAACCAACTATATGCCTCTGCTCTAGAAAAAATAATTGATAAATCT
TGGAAAGAGGCCATAGAAATGGAATCAATAGAGCCACTAAGTGAACCGGAATTAGTTGATGGATTTGAATCATTACTTAA
GAATTTCAATCCGGAAAAAACTTTAAAAATTAATCTTCAAACTGATGTTGCACCCACATTAAAATTAAAAAAATCAAAAG
GCCTATCAGTTGAGATTATAAAAAGAAAATTTGACCCCAAATCCATAGATGAAGCTTTAGAAAAATCAAGAAATCAACTA
GCAAATATTATTCCAGTTATTAATAGACCCGCTAGATTAGGAGATATCGCTGTAGTGAGTTTCAAAGGCATTTATAAAGA
TTCCAAAAAAGCAATCGATGGAGGATCTAGTGACTCAATGGATCTTGAATTAGAAAAGAACAAAATGATTCCAGGGTTCG
TTGAAGGTATTGTTGGTATGAAAATCGATGATAATAAAACTCTTAATCTCAAATTTCCTGATGACTACTCTCATGAAGAG
TCCAGAGGGAAAGAAGCAATCTTTGAAGTCAGTCTAAAAGACCTTAAAGAGAAAGAATTGCCTGAACTAAATGATGATTT
TGCAAAACAGTCTGGAAATAAAGATTCATTAAAGGAACTTAAAAAAGATATTGAAAAACAATTAAAGGAAAATTTTGATA
ACACTCAAAAAAATATCAAAATTGAAGCTTTAATGGATGCATTATCCAAAGAATTAGATGCTGAGATTCCAAAAGCAATG
ATTGATATTGAAGTAAGAAATAATATTGAACAAACAGCGCAAAGATTTGCCCAACAAGGAATGGATATCAAATCAACTTT
TACCCCGGAATTAGTTAAAACTTTAGCAGAATCAACTCGTCCTCAAGCTGAAAAAAATGTTCAAAGAAATTTAGCTTTAA
AAGCATTATCAGAAAGTGAGAATATTACAGTCGAGGATAAAGAAATAAATCAAAAAATGAAGGAATATGATGATGAAATT
TCTAAATCACAAAAACAAATTGACATTCAGAAATTAAAAGAAGTAGTTCTTAACGATCTTCTAAAAGAAAAGTTAATAAC
TTGGCTTGAAGAAAATTCAACGGTTAAAGAAATAAGTGAAAAAGTAACAAAATCAACTACAAAATCAAAGACAAAATCAA
AGACAAAGAAAGAAAGCCAAGCTAAAAGTGAACCGAATAAAAAGAAAAAAGAAAAAAAATAA

Upstream 100 bases:

>100_bases
ATATTTCTCTTACTGTATCAAAAAAGTTAACTTCAAGGAAATCACTTAATGTGGAATAATTAAGTTTCTGCTTATTGTAA
TAATTTAAAAAAACTCAAAC

Downstream 100 bases:

>100_bases
TTTATAAAAAATTTAACTAATCAAGAAAAAAACTTCTAAATTAGATATAAAAGAGATTAATTTAGTGAATTCTAAACAAA
AACATTTAATACACAGCTCA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIKEALIVKTTALPQSRIALELEIPSNTCKSFVSETINSISRSAKIPGFRLGKIPKQVLIQRIGINQLYASALEKIIDKS
WKEAIEMESIEPLSEPELVDGFESLLKNFNPEKTLKINLQTDVAPTLKLKKSKGLSVEIIKRKFDPKSIDEALEKSRNQL
ANIIPVINRPARLGDIAVVSFKGIYKDSKKAIDGGSSDSMDLELEKNKMIPGFVEGIVGMKIDDNKTLNLKFPDDYSHEE
SRGKEAIFEVSLKDLKEKELPELNDDFAKQSGNKDSLKELKKDIEKQLKENFDNTQKNIKIEALMDALSKELDAEIPKAM
IDIEVRNNIEQTAQRFAQQGMDIKSTFTPELVKTLAESTRPQAEKNVQRNLALKALSESENITVEDKEINQKMKEYDDEI
SKSQKQIDIQKLKEVVLNDLLKEKLITWLEENSTVKEISEKVTKSTTKSKTKSKTKKESQAKSEPNKKKKEKK

Sequences:

>Translated_473_residues
MIKEALIVKTTALPQSRIALELEIPSNTCKSFVSETINSISRSAKIPGFRLGKIPKQVLIQRIGINQLYASALEKIIDKS
WKEAIEMESIEPLSEPELVDGFESLLKNFNPEKTLKINLQTDVAPTLKLKKSKGLSVEIIKRKFDPKSIDEALEKSRNQL
ANIIPVINRPARLGDIAVVSFKGIYKDSKKAIDGGSSDSMDLELEKNKMIPGFVEGIVGMKIDDNKTLNLKFPDDYSHEE
SRGKEAIFEVSLKDLKEKELPELNDDFAKQSGNKDSLKELKKDIEKQLKENFDNTQKNIKIEALMDALSKELDAEIPKAM
IDIEVRNNIEQTAQRFAQQGMDIKSTFTPELVKTLAESTRPQAEKNVQRNLALKALSESENITVEDKEINQKMKEYDDEI
SKSQKQIDIQKLKEVVLNDLLKEKLITWLEENSTVKEISEKVTKSTTKSKTKSKTKKESQAKSEPNKKKKEKK
>Mature_473_residues
MIKEALIVKTTALPQSRIALELEIPSNTCKSFVSETINSISRSAKIPGFRLGKIPKQVLIQRIGINQLYASALEKIIDKS
WKEAIEMESIEPLSEPELVDGFESLLKNFNPEKTLKINLQTDVAPTLKLKKSKGLSVEIIKRKFDPKSIDEALEKSRNQL
ANIIPVINRPARLGDIAVVSFKGIYKDSKKAIDGGSSDSMDLELEKNKMIPGFVEGIVGMKIDDNKTLNLKFPDDYSHEE
SRGKEAIFEVSLKDLKEKELPELNDDFAKQSGNKDSLKELKKDIEKQLKENFDNTQKNIKIEALMDALSKELDAEIPKAM
IDIEVRNNIEQTAQRFAQQGMDIKSTFTPELVKTLAESTRPQAEKNVQRNLALKALSESENITVEDKEINQKMKEYDDEI
SKSQKQIDIQKLKEVVLNDLLKEKLITWLEENSTVKEISEKVTKSTTKSKTKSKTKKESQAKSEPNKKKKEKK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=335, Percent_Identity=28.3582089552239, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PROM5 (A2BZ41)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012159.1
- ProteinModelPortal:   A2BZ41
- STRING:   A2BZ41
- GeneID:   4720423
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18451
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   PMAR167542:P9515ORF_1927-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 53559; Mature: 53559

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEALIVKTTALPQSRIALELEIPSNTCKSFVSETINSISRSAKIPGFRLGKIPKQVLI
CCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHH
QRIGINQLYASALEKIIDKSWKEAIEMESIEPLSEPELVDGFESLLKNFNPEKTLKINLQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
TDVAPTLKLKKSKGLSVEIIKRKFDPKSIDEALEKSRNQLANIIPVINRPARLGDIAVVS
CCCCCHHEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
FKGIYKDSKKAIDGGSSDSMDLELEKNKMIPGFVEGIVGMKIDDNKTLNLKFPDDYSHEE
EHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCEEECCCCEEEEECCCCCCCHH
SRGKEAIFEVSLKDLKEKELPELNDDFAKQSGNKDSLKELKKDIEKQLKENFDNTQKNIK
HCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCH
IEALMDALSKELDAEIPKAMIDIEVRNNIEQTAQRFAQQGMDIKSTFTPELVKTLAESTR
HHHHHHHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PQAEKNVQRNLALKALSESENITVEDKEINQKMKEYDDEISKSQKQIDIQKLKEVVLNDL
CHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEKLITWLEENSTVKEISEKVTKSTTKSKTKSKTKKESQAKSEPNKKKKEKK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MIKEALIVKTTALPQSRIALELEIPSNTCKSFVSETINSISRSAKIPGFRLGKIPKQVLI
CCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHH
QRIGINQLYASALEKIIDKSWKEAIEMESIEPLSEPELVDGFESLLKNFNPEKTLKINLQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
TDVAPTLKLKKSKGLSVEIIKRKFDPKSIDEALEKSRNQLANIIPVINRPARLGDIAVVS
CCCCCHHEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
FKGIYKDSKKAIDGGSSDSMDLELEKNKMIPGFVEGIVGMKIDDNKTLNLKFPDDYSHEE
EHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCEEECCCCEEEEECCCCCCCHH
SRGKEAIFEVSLKDLKEKELPELNDDFAKQSGNKDSLKELKKDIEKQLKENFDNTQKNIK
HCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCH
IEALMDALSKELDAEIPKAMIDIEVRNNIEQTAQRFAQQGMDIKSTFTPELVKTLAESTR
HHHHHHHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PQAEKNVQRNLALKALSESENITVEDKEINQKMKEYDDEISKSQKQIDIQKLKEVVLNDL
CHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEKLITWLEENSTVKEISEKVTKSTTKSKTKSKTKKESQAKSEPNKKKKEKK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA