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The map label for this gene is miaA

Identifier: 123967057

GI number: 123967057

Start: 1607148

End: 1608047

Strand: Direct

Name: miaA

Synonym: P9515_18241

Alternate gene names: 123967057

Gene position: 1607148-1608047 (Clockwise)

Preceding gene: 123967050

Following gene: 123967058

Centisome position: 94.31

GC content: 31.0

Gene sequence:

>900_bases
ATGCTTCCTTCAAAACCACTAGTAATAGTTTTAATCGGGCCGACAGCAAGTGGCAAAACTGAGCTAGCAATAGATATCGC
TAAATATTTTAATATCCATATACACAATGTAGATTCTAGACAAATTTATAGATTTATGGACATCGGCACAGCTAAACCAA
CGAAAGTTCAACAAAGAGCTATAAAGCATTTTTTAATAGATGTTGAAGATCCCTCAGTAAAAGTAAATGCAAAGCAATTT
CAAGAAATTGCCACAAAATCAATAAATCGAGAATTAAATCAGAAAAAAACACCCTTTCTAGTTGGTGGTAGCGGATTATA
TATGAATTCAATAATAAAGGGATTTTTTGCACCAGATGTTCCTCCTCAAAGTTTTCTGAGGTCCCAATTCGAAAAATTAG
GTCAAGAAAAATGTTGGGAACTTCTAAAAGTTTGTGACCCTGAATTAACAAAAACAATTAATTATGCCGATCAAATAAGA
ACCATAAGAGGTTTAGAAGTCTTTTATGTAACTGGTAAAAGAATGTCATCTCAAAGATTCCAAAACCCTCCTCCATGGAG
AATCTTAGAATTGGGTATAAATAGAGTAGATTTAAAGGAAAGAATTTTCAAAAGAACAAAAAACATGTTTGAATTTGGAA
TTATAGAGGAGACCAAAAATATCATTAATCAATATGGATCGACTTTGCCTTTACTAGAAACGATTGGATATAAAGAGGCA
AAAAATGTAATCAAAGAAAATTTAACAATTGAAGAAGCTATTGAATTAACAACAACTAAAACAATCCAATTTGCTAAAAG
ACAAAAAACATGGTTCCGTAATAAGAACAATGCAATATGGCTTAACAACAAAAACCTACTAAAAGATGCAATAATTAAGA
TAGAGTATGCTTTAGGTTAA

Upstream 100 bases:

>100_bases
TCATTAAATTTACCATCGGTATAAGAGAAAGGCTTGAAGTAAATGCAAAATTTAATCACTTTAATTATATGAATCAATTA
TTTCTTTTCAAGAGTCACAC

Downstream 100 bases:

>100_bases
CTTTATAAAGTATTAGATCATGTTCATCATCAAAAAATCATTTTAAGAAACTGAATGCCACCACGTCCACGCTTTGATCG
CCGAGCTCCCGTTAGAGAGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLPSKPLVIVLIGPTASGKTELAIDIAKYFNIHIHNVDSRQIYRFMDIGTAKPTKVQQRAIKHFLIDVEDPSVKVNAKQF
QEIATKSINRELNQKKTPFLVGGSGLYMNSIIKGFFAPDVPPQSFLRSQFEKLGQEKCWELLKVCDPELTKTINYADQIR
TIRGLEVFYVTGKRMSSQRFQNPPPWRILELGINRVDLKERIFKRTKNMFEFGIIEETKNIINQYGSTLPLLETIGYKEA
KNVIKENLTIEEAIELTTTKTIQFAKRQKTWFRNKNNAIWLNNKNLLKDAIIKIEYALG

Sequences:

>Translated_299_residues
MLPSKPLVIVLIGPTASGKTELAIDIAKYFNIHIHNVDSRQIYRFMDIGTAKPTKVQQRAIKHFLIDVEDPSVKVNAKQF
QEIATKSINRELNQKKTPFLVGGSGLYMNSIIKGFFAPDVPPQSFLRSQFEKLGQEKCWELLKVCDPELTKTINYADQIR
TIRGLEVFYVTGKRMSSQRFQNPPPWRILELGINRVDLKERIFKRTKNMFEFGIIEETKNIINQYGSTLPLLETIGYKEA
KNVIKENLTIEEAIELTTTKTIQFAKRQKTWFRNKNNAIWLNNKNLLKDAIIKIEYALG
>Mature_299_residues
MLPSKPLVIVLIGPTASGKTELAIDIAKYFNIHIHNVDSRQIYRFMDIGTAKPTKVQQRAIKHFLIDVEDPSVKVNAKQF
QEIATKSINRELNQKKTPFLVGGSGLYMNSIIKGFFAPDVPPQSFLRSQFEKLGQEKCWELLKVCDPELTKTINYADQIR
TIRGLEVFYVTGKRMSSQRFQNPPPWRILELGINRVDLKERIFKRTKNMFEFGIIEETKNIINQYGSTLPLLETIGYKEA
KNVIKENLTIEEAIELTTTKTIQFAKRQKTWFRNKNNAIWLNNKNLLKDAIIKIEYALG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=25.796178343949, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790613, Length=293, Percent_Identity=32.0819112627986, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25144712, Length=265, Percent_Identity=26.7924528301887, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=26.5734265734266, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=28.4644194756554, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROM5 (A2BZ20)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012138.1
- ProteinModelPortal:   A2BZ20
- SMR:   A2BZ20
- STRING:   A2BZ20
- GeneID:   4719993
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18241
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   PMAR167542:P9515ORF_1905-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34493; Mature: 34493

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSKPLVIVLIGPTASGKTELAIDIAKYFNIHIHNVDSRQIYRFMDIGTAKPTKVQQRA
CCCCCCEEEEEECCCCCCCCEEEEEEEHHEEEEEECCCHHHHHHHHHCCCCCCHHHHHHH
IKHFLIDVEDPSVKVNAKQFQEIATKSINRELNQKKTPFLVGGSGLYMNSIIKGFFAPDV
HHHHEEECCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCC
PPQSFLRSQFEKLGQEKCWELLKVCDPELTKTINYADQIRTIRGLEVFYVTGKRMSSQRF
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHC
QNPPPWRILELGINRVDLKERIFKRTKNMFEFGIIEETKNIINQYGSTLPLLETIGYKEA
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHH
KNVIKENLTIEEAIELTTTKTIQFAKRQKTWFRNKNNAIWLNNKNLLKDAIIKIEYALG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEEEECC
>Mature Secondary Structure
MLPSKPLVIVLIGPTASGKTELAIDIAKYFNIHIHNVDSRQIYRFMDIGTAKPTKVQQRA
CCCCCCEEEEEECCCCCCCCEEEEEEEHHEEEEEECCCHHHHHHHHHCCCCCCHHHHHHH
IKHFLIDVEDPSVKVNAKQFQEIATKSINRELNQKKTPFLVGGSGLYMNSIIKGFFAPDV
HHHHEEECCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCC
PPQSFLRSQFEKLGQEKCWELLKVCDPELTKTINYADQIRTIRGLEVFYVTGKRMSSQRF
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHC
QNPPPWRILELGINRVDLKERIFKRTKNMFEFGIIEETKNIINQYGSTLPLLETIGYKEA
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHH
KNVIKENLTIEEAIELTTTKTIQFAKRQKTWFRNKNNAIWLNNKNLLKDAIIKIEYALG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA