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The map label for this gene is kefB [H]

Identifier: 123967020

GI number: 123967020

Start: 1573003

End: 1574370

Strand: Reverse

Name: kefB [H]

Synonym: P9515_17871

Alternate gene names: 123967020

Gene position: 1574370-1573003 (Counterclockwise)

Preceding gene: 123967022

Following gene: 123967019

Centisome position: 92.38

GC content: 39.18

Gene sequence:

>1368_bases
ATGTATTCTTTACTTGCTGAATTAAGTGCACATGATTTAGAAGTTGCTGAGACGCTTATAGGAGTTATTAGATTTCTATT
AATCTTTTTAGCTGCAAGAGCATTAGCCGAAGTGTTAGTAAGACTAAGTTTACCTACAATTGTGGGTGAGCTATTGGCAG
GGGTTGTTATAGGAGCATCTGGTTTTCATTTACTTATACCACCCTCTGCTCATACTGAATTAAATCAAGGGTTAGTCAAT
GTAATAAGTTCGTTAGCGTCAATACCCCCTGAGGTTGTTCCTGACGTTTATTTCGAGAGTTTTCCCTCTCTTCAAGCGGT
TGCAACACTTGGATTATATGCGTTACTTTTCTTGACAGGCTTAGAAAGCGAATTAGAAGAATTAGTTGCAGTTGGAGCTC
AAGCTTTTACTGTTGCTATGGCGGGGGTTATATTACCTTTTGCTTTTGGTACTTTTGGATTAATGTTTATTTTTCAGGTA
GATCTGATACCTGCAGTTTTTGCAGGAGCATCGATGACGGCTACAAGTATAGGTATTACAGCAAGTGTTTTTGGTGAATT
AGGATATTTGAAAACCAGGGAGGGCCAAATTGTTATTGGAGCCGCAGTATTAGATGATATTTTAGGTATTGTTATTTTGG
CTGTAGTTGTAGCTCTTGCTGCTGGAGGTTCTCTAGAAATCGCTCCAATAGTTAAATTAGTTGCTGCGGCTGTTGTATTT
GTAATCGCAGCTATTGCATTAAGTCGAACAGCAGCTCCTGGCTTTGATTGGCTTCTCGATAGGTTGAAAGCACCAGGAGC
AGTAGTTGTGGCATCTTTTGTGATACTTGTGTTATGTTGTTTTGTCGCTACAGCGATAGGACTTGAAGCTGCTTTGGGTG
CATTCGCAGCTGGTTTGATTCTTAGTAGTTCTAAAAATAATCATGCAATTCAACAATCAGTTTTGCCGTTGGTTTCATTA
TTTGCGACTATCTTTTTTGTATTAGTTGGGGCTGGTATGGATTTATCAGTAATCAATCCACTAGATCCAGCAAGTAGATC
CGCGCTTATAGTTGCAGGGTTTTTATTAGTTGTAGCAATTATTGGAAAAATTGCTGCTGGATGGGTTTTCTCAAGTGATA
AGCCTACAAATAGATTAGTAGTAGGTTTAGGAATGATGCCAAGAGGAGAAGTCGGGTTAATTTTTCTTGGTTTAGGTACC
AGTGCTGGCCTATTGACCCCCTCTCTTGAAGCGGCAATTTTATTGATGGTTATTGGAACTACATTCCTAGCTCCGGTTCT
TTTAAGAATTGTTTTAAAAGATAAGCCCCCCGGTGGTGATAATAAAATTTCAGATGATGTTGCAGCTGATCCAGTAGGTC
TTCTTTAA

Upstream 100 bases:

>100_bases
ACACTTGTTGAGTATTTTTTCTTTTTTTTACAGACTTCGTAAAGTGGGCCCATCATAAACTATCTTCCAATTAGATAGTT
CAATTTTTCTCGCATTTAAA

Downstream 100 bases:

>100_bases
GGAATAATTTTATTAGAATTATAAGAAATTAATAACTGTATAAATGGACAACTCTTTATTAATAGAAAATCAAGTTAATT
TTTCTTGGAATTTTTTAAAT

Product: CPA2 family Na+/H+ antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MYSLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAHTELNQGLVN
VISSLASIPPEVVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGSLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPASRSALIVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAGLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPGGDNKISDDVAADPVGLL

Sequences:

>Translated_455_residues
MYSLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAHTELNQGLVN
VISSLASIPPEVVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGSLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPASRSALIVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAGLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPGGDNKISDDVAADPVGLL
>Mature_455_residues
MYSLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAHTELNQGLVN
VISSLASIPPEVVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGSLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPASRSALIVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAGLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPGGDNKISDDVAADPVGLL

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=443, Percent_Identity=24.6049661399549, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322367, Length=452, Percent_Identity=22.787610619469, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 46736; Mature: 46736

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFHLLIPPSAHTELNQGLVNVISSLASIPPEVVPDVYFESFPSLQAVATLGLYALLFLTG
CCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
LESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQVDLIPAVFAGASMTATSIGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGSLEIAPIVKLVAAAVVF
HHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLI
HHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPASRSALIVAGFLLVVAI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLTPSLEAAILLMVIGT
HHHHHHCCEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
TFLAPVLLRIVLKDKPPGGDNKISDDVAADPVGLL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYSLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFHLLIPPSAHTELNQGLVNVISSLASIPPEVVPDVYFESFPSLQAVATLGLYALLFLTG
CCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
LESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQVDLIPAVFAGASMTATSIGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGSLEIAPIVKLVAAAVVF
HHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLI
HHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPASRSALIVAGFLLVVAI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLTPSLEAAILLMVIGT
HHHHHHCCEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
TFLAPVLLRIVLKDKPPGGDNKISDDVAADPVGLL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]