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The map label for this gene is thiF [H]

Identifier: 123966989

GI number: 123966989

Start: 1541555

End: 1542703

Strand: Direct

Name: thiF [H]

Synonym: P9515_17561

Alternate gene names: 123966989

Gene position: 1541555-1542703 (Clockwise)

Preceding gene: 123966987

Following gene: 123966991

Centisome position: 90.46

GC content: 28.46

Gene sequence:

>1149_bases
ATGAAAATATCAATTGATCACAAATCTGATTCCTTAACTTTAGATGAAGAAGATAGATACAAGAGACATTTAACACTCAA
TGAAATAGGATTAAAGGGACAATTAAAACTTAAACGCAGTTCAGTAGTTTGTATTGGCGCAGGAGGTTTAGGCTCTTCTG
TATTAATTTATCTTGCCGCTGCAGGAATTGGAACAATAGGAATAGTTGATAATGATCAAGTTGAGAAGTCGAATCTACAA
AGACAAATAATTCATGAAACAAATACAGTTGGGGATTTAAAAATTGATTCTGCTCAAGAAAGAATTAGAAGATTGAATCC
TAATATTGAAGTAATAACTTTTGCTGAACGAATTAACTCAAATAATATTCTCGATATTATTAATCAATTTGATATTGTTT
GTGATTGTTCAGATAACTTTGGTACTCGTTATTTAATAAACGATGCTTGCTTAATACTTGATAAACCTTTAGTTTTTGGA
AGCGTTCAAGGATTTGAAGGCCAAATCAGTGTTTTTAATCTAAAAAAAAATAGTCCCAATTTAAGAGACTTACTCCCGGA
ATCGCCTTTAAAAAATAATATTCCTAGCTGCGAAGAATTTGGCGTTATAGGAGTTTCAACTGGTCTTATAGGAGTTTTAC
AAGCAAATGAAGCAATAAAGATTATTCTTAAAAAAGGGCAAATTCTTGATGGGAAGATTTTGATTTTCAACCTTCTCAAT
ATGAATATAAAAACATTGACTTTAAAAGCTGATAAATTTACAAATACGATTAATGACCTTTCTGAGTTTGAAGATTTTTA
TAAAGACATTGAATGTCAAGATAATATAAAAATTAATAAAATAGATTCCACGACATTTGAAACACTATACAGATCAAATT
ATAATAATCTACTAATAATAGATGTTAGAGAAAAAGAGGAATTTAATAAATACTCTATTAAAGGAGCGATATCTATACCA
CTTAATAATCTAGACCAAAAACCACACCTAGAATTTATCAAACAAGAAAGTTTGGATAAAGAAGTATTTACATTATGTCA
AGCAGGAAAACGATCTGAAAAAGCTTCAAAGATTTTGATGAAATTTAAAATCTCATCAAAATCAATTGAAGGAGGAATTG
CAAATATTAAACAACTAATTTTTCATTAA

Upstream 100 bases:

>100_bases
AACAAAAAACATTAAAAAACCACATCAACAGAAAAAAATAAAAAGTCTAAAATAATTTTTTAAATTATTATATTTTCATA
GATTATTTATAAAAACAAGA

Downstream 100 bases:

>100_bases
CATACTGAATAAACTTTAATAATCTACCCCTCTTTTTAAATCAACTCCAAGATTCGCATAATGCTTGTGACAAACCATTT
CAGAATATACATCAGCTAAT

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKISIDHKSDSLTLDEEDRYKRHLTLNEIGLKGQLKLKRSSVVCIGAGGLGSSVLIYLAAAGIGTIGIVDNDQVEKSNLQ
RQIIHETNTVGDLKIDSAQERIRRLNPNIEVITFAERINSNNILDIINQFDIVCDCSDNFGTRYLINDACLILDKPLVFG
SVQGFEGQISVFNLKKNSPNLRDLLPESPLKNNIPSCEEFGVIGVSTGLIGVLQANEAIKIILKKGQILDGKILIFNLLN
MNIKTLTLKADKFTNTINDLSEFEDFYKDIECQDNIKINKIDSTTFETLYRSNYNNLLIIDVREKEEFNKYSIKGAISIP
LNNLDQKPHLEFIKQESLDKEVFTLCQAGKRSEKASKILMKFKISSKSIEGGIANIKQLIFH

Sequences:

>Translated_382_residues
MKISIDHKSDSLTLDEEDRYKRHLTLNEIGLKGQLKLKRSSVVCIGAGGLGSSVLIYLAAAGIGTIGIVDNDQVEKSNLQ
RQIIHETNTVGDLKIDSAQERIRRLNPNIEVITFAERINSNNILDIINQFDIVCDCSDNFGTRYLINDACLILDKPLVFG
SVQGFEGQISVFNLKKNSPNLRDLLPESPLKNNIPSCEEFGVIGVSTGLIGVLQANEAIKIILKKGQILDGKILIFNLLN
MNIKTLTLKADKFTNTINDLSEFEDFYKDIECQDNIKINKIDSTTFETLYRSNYNNLLIIDVREKEEFNKYSIKGAISIP
LNNLDQKPHLEFIKQESLDKEVFTLCQAGKRSEKASKILMKFKISSKSIEGGIANIKQLIFH
>Mature_382_residues
MKISIDHKSDSLTLDEEDRYKRHLTLNEIGLKGQLKLKRSSVVCIGAGGLGSSVLIYLAAAGIGTIGIVDNDQVEKSNLQ
RQIIHETNTVGDLKIDSAQERIRRLNPNIEVITFAERINSNNILDIINQFDIVCDCSDNFGTRYLINDACLILDKPLVFG
SVQGFEGQISVFNLKKNSPNLRDLLPESPLKNNIPSCEEFGVIGVSTGLIGVLQANEAIKIILKKGQILDGKILIFNLLN
MNIKTLTLKADKFTNTINDLSEFEDFYKDIECQDNIKINKIDSTTFETLYRSNYNNLLIIDVREKEEFNKYSIKGAISIP
LNNLDQKPHLEFIKQESLDKEVFTLCQAGKRSEKASKILMKFKISSKSIEGGIANIKQLIFH

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=382, Percent_Identity=34.2931937172775, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4885649, Length=157, Percent_Identity=29.2993630573248, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI23510338, Length=167, Percent_Identity=29.3413173652695, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI23510340, Length=167, Percent_Identity=29.3413173652695, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38045948, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787048, Length=230, Percent_Identity=36.5217391304348, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87082356, Length=233, Percent_Identity=38.1974248927039, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17540406, Length=375, Percent_Identity=33.6, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=141, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=390, Percent_Identity=34.1025641025641, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322639, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325438, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320598, Length=148, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582879, Length=363, Percent_Identity=36.6391184573003, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24660640, Length=136, Percent_Identity=32.3529411764706, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573937, Length=190, Percent_Identity=28.4210526315789, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 42853; Mature: 42853

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISIDHKSDSLTLDEEDRYKRHLTLNEIGLKGQLKLKRSSVVCIGAGGLGSSVLIYLAA
CEEEEECCCCCEEECCCHHHHHEEEHHHCCCCEEEEEEECCEEEEECCCCCHHHHHHHHH
AGIGTIGIVDNDQVEKSNLQRQIIHETNTVGDLKIDSAQERIRRLNPNIEVITFAERINS
CCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCEEEEEEEHHCCC
NNILDIINQFDIVCDCSDNFGTRYLINDACLILDKPLVFGSVQGFEGQISVFNLKKNSPN
CCHHHHHCCCEEEEECCCCCCCEEEECCEEEEEECCEEEEECCCCCCEEEEEEEECCCCC
LRDLLPESPLKNNIPSCEEFGVIGVSTGLIGVLQANEAIKIILKKGQILDGKILIFNLLN
HHHHCCCCCCCCCCCCHHHCCEEEECCCEEEEEECCCEEEEEEECCCEECCEEEEEEECC
MNIKTLTLKADKFTNTINDLSEFEDFYKDIECQDNIKINKIDSTTFETLYRSNYNNLLII
CCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEE
DVREKEEFNKYSIKGAISIPLNNLDQKPHLEFIKQESLDKEVFTLCQAGKRSEKASKILM
EECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHEEEE
KFKISSKSIEGGIANIKQLIFH
EEEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKISIDHKSDSLTLDEEDRYKRHLTLNEIGLKGQLKLKRSSVVCIGAGGLGSSVLIYLAA
CEEEEECCCCCEEECCCHHHHHEEEHHHCCCCEEEEEEECCEEEEECCCCCHHHHHHHHH
AGIGTIGIVDNDQVEKSNLQRQIIHETNTVGDLKIDSAQERIRRLNPNIEVITFAERINS
CCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCEEEEEEEHHCCC
NNILDIINQFDIVCDCSDNFGTRYLINDACLILDKPLVFGSVQGFEGQISVFNLKKNSPN
CCHHHHHCCCEEEEECCCCCCCEEEECCEEEEEECCEEEEECCCCCCEEEEEEEECCCCC
LRDLLPESPLKNNIPSCEEFGVIGVSTGLIGVLQANEAIKIILKKGQILDGKILIFNLLN
HHHHCCCCCCCCCCCCHHHCCEEEECCCEEEEEECCCEEEEEEECCCEECCEEEEEEECC
MNIKTLTLKADKFTNTINDLSEFEDFYKDIECQDNIKINKIDSTTFETLYRSNYNNLLII
CCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEE
DVREKEEFNKYSIKGAISIPLNNLDQKPHLEFIKQESLDKEVFTLCQAGKRSEKASKILM
EECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHEEEE
KFKISSKSIEGGIANIKQLIFH
EEEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]