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The map label for this gene is tufA [H]

Identifier: 123966921

GI number: 123966921

Start: 1483880

End: 1485079

Strand: Reverse

Name: tufA [H]

Synonym: P9515_16881

Alternate gene names: 123966921

Gene position: 1485079-1483880 (Counterclockwise)

Preceding gene: 123966922

Following gene: 123966920

Centisome position: 87.14

GC content: 39.5

Gene sequence:

>1200_bases
ATGGCTCGCGAGAAGTTCGAAAGGAACAAACCACATGTCAACATAGGTACTATCGGCCACGTTGATCATGGAAAAACAAC
CCTGACTGCTGCTATTACCAATGTTTTAGCTAAAAAAGGTCAAGCACAAGCTCAAGATTATGGAGACATTGATGGTGCTC
CAGAAGAAAGAGAACGTGGCATAACAATTAATACAGCCCACGTTGAATACGAAACTGAAGGCAGACATTATGCCCACGTT
GATTGTCCAGGACATGCTGATTATGTCAAAAACATGATCACTGGAGCGGCTCAAATGGATGGTGCAATTTTAGTTTGTGC
TGCCACAGATGGTCCTATGGCACAAACAAAAGAACATATCCTTTTAGCAAAACAAGTTGGTGTTCCTGCTTTAGTTGTCG
CTTTAAATAAATGTGACATGGTAGATGACGAAGAAATCATTGAACTTGTGGAAATGGAAATCAGAGAGCTACTTGATAGT
TATGATTTCCCCGGGGATGATATACCTATCGTCCAGGTTTCAGGCCTGAAAGCTTTGGAAGGAGATTCTAATTGGGAATC
TAAAATTGAAGAATTAATGAAAGCTGTTGATGCTAGTATTCCTGAACCAGAAAGAGAGATTGACAAGCCATTTTTGATGG
CAATAGAAGATGTTTTCTCTATTACAGGTAGAGGAACAGTTGCTACAGGAAGAATAGAAAGAGGTAAAGTCAAAGTTGGA
GAAGAAGTCGAAATAGTTGGGATAAGAGATACAAGATTGACCACTGTTACTGGTGTTGAGATGTTCCGTAAACTTCTTGA
TGAAGGTATGGCTGGAGACAATGTTGGACTACTCCTACGTGGTGTTCAAAAAGAAGATATTGAGAGAGGAATGGTACTTG
TTAAGAAAGGTTCAATTACTCCTCATACTAAATTTGAAGGAGAAGTTTATGTTCTTAAGAAAGAAGAGGGAGGGAGACAT
ACTCCTTTCTTTGCTGGTTATAGACCACAATTTTATATAAGAACCACAGATGTGACTGGACAAATCACCGCATTCACCTC
TGATGATGGAGCTAATGTAGAAATGGTAATGCCAGGAGACAGAATAAAAATGACTGGTGAATTAATTTGTCCAGTTGCTA
TTGAACAAGGTATGCGCTTTGCTATTCGCGAAGGTGGTCGTACCATTGGAGCTGGTGTTGTTTCTAAAATTATTGAATAA

Upstream 100 bases:

>100_bases
GAAGTTCCTCGTAATGTTGCTGAAGCTATCATTTCCAAGAATCAGGGCAACTCCTGATCTCTAAACTAAACACTCTTTAA
CCCTCGATTCTTTAATTCAA

Downstream 100 bases:

>100_bases
ATTTCTTTAATTTTATGAAGTTAACCTCTGATAATCTAGAATAATTAAATAAGAGATACGGGTTATTGATTATCAATAAC
CCTTTTATTAAGAGATCAAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDS
YDFPGDDIPIVQVSGLKALEGDSNWESKIEELMKAVDASIPEPEREIDKPFLMAIEDVFSITGRGTVATGRIERGKVKVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGANVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Sequences:

>Translated_399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDS
YDFPGDDIPIVQVSGLKALEGDSNWESKIEELMKAVDASIPEPEREIDKPFLMAIEDVFSITGRGTVATGRIERGKVKVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGANVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
>Mature_398_residues
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERGITINTAHVEYETEGRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSY
DFPGDDIPIVQVSGLKALEGDSNWESKIEELMKAVDASIPEPEREIDKPFLMAIEDVFSITGRGTVATGRIERGKVKVGE
EVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRHT
PFFAGYRPQFYIRTTDVTGQITAFTSDDGANVEMVMPGDRIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=53.8847117794486, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=30.5494505494506, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503471, Length=462, Percent_Identity=30.7359307359307, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.1587301587302, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.1587301587302, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=28.7925696594427, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=28.735632183908, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=27.2935779816514, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI194018522, Length=435, Percent_Identity=27.1264367816092, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=27.2935779816514, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.4644194756554, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=25.1824817518248, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=32.4786324786325, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=32.4786324786325, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=73.1155778894472, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=73.1155778894472, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=334, Percent_Identity=32.3353293413174, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789108, Length=336, Percent_Identity=30.952380952381, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=32.3651452282158, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=24.8484848484848, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=406, Percent_Identity=49.7536945812808, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.8974358974359, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=327, Percent_Identity=35.7798165137615, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=327, Percent_Identity=35.7798165137615, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=29.3181818181818, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=29.3577981651376, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=312, Percent_Identity=32.0512820512821, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=27.7777777777778, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=31.7343173431734, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=28.8793103448276, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=26.7657992565056, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17565438, Length=437, Percent_Identity=23.5697940503432, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=177, Percent_Identity=35.5932203389831, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.4685138539043, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=31.4606741573034, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=31.4606741573034, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=452, Percent_Identity=25.6637168141593, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=441, Percent_Identity=28.1179138321995, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=273, Percent_Identity=27.8388278388278, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=29.8755186721992, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=32.5925925925926, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=56.7088607594937, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=56.7088607594937, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=46.0358056265985, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=29.9776286353468, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=29.9776286353468, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=29.9776286353468, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=29.9776286353468, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.954954954955, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.954954954955, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.3630363036304, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=27.4336283185841, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17137380, Length=452, Percent_Identity=28.0973451327434, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647048, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78706572, Length=260, Percent_Identity=26.5384615384615, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=34.7560975609756, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43678; Mature: 43547

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSYDFPGDDIPIVQVSGLKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GDSNWESKIEELMKAVDASIPEPEREIDKPFLMAIEDVFSITGRGTVATGRIERGKVKVG
CCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCCEECC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGANVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCCCEEEEEECCC
RIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEECCCEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQAQDYGDIDGAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLDSYDFPGDDIPIVQVSGLKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GDSNWESKIEELMKAVDASIPEPEREIDKPFLMAIEDVFSITGRGTVATGRIERGKVKVG
CCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCCEECC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGVQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGANVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCCCEEEEEECCC
RIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEECCCEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA