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The map label for this gene is rne [H]

Identifier: 123966914

GI number: 123966914

Start: 1477999

End: 1479825

Strand: Direct

Name: rne [H]

Synonym: P9515_16811

Alternate gene names: 123966914

Gene position: 1477999-1479825 (Clockwise)

Preceding gene: 123966911

Following gene: 123966915

Centisome position: 86.73

GC content: 30.82

Gene sequence:

>1827_bases
ATGTCTCAGCAAATTATAATAGCTGAGCAGGCTCGTATTGCAGCACTACTCACGGATGATCGAGTTGATGAATTAATCGT
CGCACAAGGTCAATATCAAATCGGGGATATTTTTTTAGGAACTGTTGAAAATGTTCTACCAGGAATTGATGCTGCTTTTA
TCCATATTGGAGAAAGTGAAAAAAATGGCTTTATTCATGTATCAGATCTAGGTCCACTAAGACTGAAAAAAGGTGTTGTA
GGCATCACTGAGCTTCTTGAACCCAAACAAAAAGTTCTCGTTCAAGTAATGAAGGAACCTACTGGAAATAAAGGGCCCAG
ACTAACAGGTAATATTTCATTACCTGGTAAATATTTAATACTGCAACCTTTCGGACAAGGTGTGAATATTTCTAGGAAGA
TAAATACTGATACTGAAAGAAGTCGTTTAAGAGCACTTGGAGTCTTAATTAAACCACCTAGCACAGGTCTATTATTTCGA
ACAGAAGCCGAAAAAATAAAAGAAGAATTACTTATTGAAGATCTGGAAAATTTAATTCAACAATGGGATCAAGTTTTAAA
AATTTCAGAGACTTGTCAACCACCAAATTTAATAAATAGAGATGAAGATTTTTCATTAAAAATTTTAAGAGACTGTATTA
AAGCATCAACTAGTAAAGTTGTAATTGATAATAAAGTAGCTCTCGAAAAAGCAAAAGACTTTTTAATTAATAATGATTCT
AATGTGGATCTTGTCTTACATAATAATGACATAAATGAACATATTCTTGAAAAATATCAAATTAATAAAACTATTCAAAA
GGCACTTGAACCAAGAGTAGATTTACCCTCAGGTGGATATATCATTATTGAGCCTACTGAAGCTTTAACCGTAATTGATG
TGAATTCCGGTTCTTTCACAAGATCAGCTAATTCAAGACAAACTGTTTTATGGACGAATTGCGAAGCAGCTGTTGAAATT
TCTCGACAAATGAAATTAAGGAATATTGGTGGAGTTTTAGTAATAGACTTTATAGATATGGAATCTCGAAGAGATCAATT
TCAGTTACTTGAGCAGTTTACTCTAGCCATAAAAGATGATTCTGCAAGACCTCAGATAGCTGAGTTAACTGAGTTAGGTT
TAGTCGAATTAACAAGAAAAAGGCAAGGTCAAAATATATATGAATTATTCGGCAAAAAATGTAATGCTTGCAATGGATTA
GGGCATATAAAAAGTTTACCAAACTATCAAAATTCGAATTCAGAAACAAATCACTCTGCAAAAAAATCTAGCAGATTAAA
TAATACAAAGTTCACAGATTTAGAGCCATCTGAAGTAATTGAAAATCAAGAAAAAATTGTCAAGAAAGAGTTACCTAACT
CCAAAATTTTAAATAAAGACGATGATCCTGCCAAACAAAAAGAAAATGATAATGAAATAGTTAATACCCATAATTCAAAA
GAAAAGAAAGTAATAACAGTTGAGCTAAGCGAAGTTGAAAAGATTGTCTATAGTCAACTTGGTATTAATCCATTAATTAA
ATTAGGAAAACAATATCTTACTAATAATCATTTAGTACGTTTAGAGAATAGTAATAACAACGAAAAAGAACTCTCTTTAC
AAAAGAATAAAAAAGCAAAAACTAAAGTTTCTAATAAGAAAACAAATAAAAAATCCTCAACTTCTAATTCAATAGAAAAG
TCTGAAGTTGAAGTAAATGAGTCTAAAGAAATTAATCATGAGAGCAAATTACCAAAGATAACAACTGCTAATGAGGAAGT
AGAAATAACAGATGAAATTGATAATTCAAGACGGAAAAGAAGAAGATCATCTGCAAGTATTGAATAA

Upstream 100 bases:

>100_bases
AATAAATTTAACAAACAAGAAATTGTTAGTAAATAATTAAATATAAAATTATTTACCTTTTATCGATTTTCAAAATTCAA
AACATACTTAATCTTTTTAT

Downstream 100 bases:

>100_bases
ACAACTTGAGATAGGTATTGACGAAGTAGGTCGAGGTGCAGTTTTTGGACCTGTATTTTCTGCAGCTGTAGTTCTAACCG
AAAAAAATGGATATACTCTC

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFIHIGESEKNGFIHVSDLGPLRLKKGVV
GITELLEPKQKVLVQVMKEPTGNKGPRLTGNISLPGKYLILQPFGQGVNISRKINTDTERSRLRALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLENLIQQWDQVLKISETCQPPNLINRDEDFSLKILRDCIKASTSKVVIDNKVALEKAKDFLINNDS
NVDLVLHNNDINEHILEKYQINKTIQKALEPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVLVIDFIDMESRRDQFQLLEQFTLAIKDDSARPQIAELTELGLVELTRKRQGQNIYELFGKKCNACNGL
GHIKSLPNYQNSNSETNHSAKKSSRLNNTKFTDLEPSEVIENQEKIVKKELPNSKILNKDDDPAKQKENDNEIVNTHNSK
EKKVITVELSEVEKIVYSQLGINPLIKLGKQYLTNNHLVRLENSNNNEKELSLQKNKKAKTKVSNKKTNKKSSTSNSIEK
SEVEVNESKEINHESKLPKITTANEEVEITDEIDNSRRKRRRSSASIE

Sequences:

>Translated_608_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFIHIGESEKNGFIHVSDLGPLRLKKGVV
GITELLEPKQKVLVQVMKEPTGNKGPRLTGNISLPGKYLILQPFGQGVNISRKINTDTERSRLRALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLENLIQQWDQVLKISETCQPPNLINRDEDFSLKILRDCIKASTSKVVIDNKVALEKAKDFLINNDS
NVDLVLHNNDINEHILEKYQINKTIQKALEPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVLVIDFIDMESRRDQFQLLEQFTLAIKDDSARPQIAELTELGLVELTRKRQGQNIYELFGKKCNACNGL
GHIKSLPNYQNSNSETNHSAKKSSRLNNTKFTDLEPSEVIENQEKIVKKELPNSKILNKDDDPAKQKENDNEIVNTHNSK
EKKVITVELSEVEKIVYSQLGINPLIKLGKQYLTNNHLVRLENSNNNEKELSLQKNKKAKTKVSNKKTNKKSSTSNSIEK
SEVEVNESKEINHESKLPKITTANEEVEITDEIDNSRRKRRRSSASIE
>Mature_607_residues
SQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFIHIGESEKNGFIHVSDLGPLRLKKGVVG
ITELLEPKQKVLVQVMKEPTGNKGPRLTGNISLPGKYLILQPFGQGVNISRKINTDTERSRLRALGVLIKPPSTGLLFRT
EAEKIKEELLIEDLENLIQQWDQVLKISETCQPPNLINRDEDFSLKILRDCIKASTSKVVIDNKVALEKAKDFLINNDSN
VDLVLHNNDINEHILEKYQINKTIQKALEPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEIS
RQMKLRNIGGVLVIDFIDMESRRDQFQLLEQFTLAIKDDSARPQIAELTELGLVELTRKRQGQNIYELFGKKCNACNGLG
HIKSLPNYQNSNSETNHSAKKSSRLNNTKFTDLEPSEVIENQEKIVKKELPNSKILNKDDDPAKQKENDNEIVNTHNSKE
KKVITVELSEVEKIVYSQLGINPLIKLGKQYLTNNHLVRLENSNNNEKELSLQKNKKAKTKVSNKKTNKKSSTSNSIEKS
EVEVNESKEINHESKLPKITTANEEVEITDEIDNSRRKRRRSSASIE

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=380, Percent_Identity=35.2631578947368, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87082235, Length=383, Percent_Identity=33.6814621409922, Blast_Score=213, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 68454; Mature: 68323

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFIHIGESE
CCCCEEEECCCEEEEEECCCCHHHHEEECCCEEECHHHHHHHHHHHCCCCEEEEEECCCC
KNGFIHVSDLGPLRLKKGVVGITELLEPKQKVLVQVMKEPTGNKGPRLTGNISLPGKYLI
CCCEEEEECCCCHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEE
LQPFGQGVNISRKINTDTERSRLRALGVLIKPPSTGLLFRTEAEKIKEELLIEDLENLIQ
EEECCCCCCEEEECCCCHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWDQVLKISETCQPPNLINRDEDFSLKILRDCIKASTSKVVIDNKVALEKAKDFLINNDS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHEECCCC
NVDLVLHNNDINEHILEKYQINKTIQKALEPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVLVIDFIDMESRRDQFQLLEQFTLAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEECC
SARPQIAELTELGLVELTRKRQGQNIYELFGKKCNACNGLGHIKSLPNYQNSNSETNHSA
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCHHH
KKSSRLNNTKFTDLEPSEVIENQEKIVKKELPNSKILNKDDDPAKQKENDNEIVNTHNSK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCEECCCCCC
EKKVITVELSEVEKIVYSQLGINPLIKLGKQYLTNNHLVRLENSNNNEKELSLQKNKKAK
CCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHH
TKVSNKKTNKKSSTSNSIEKSEVEVNESKEINHESKLPKITTANEEVEITDEIDNSRRKR
HHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCEEECCCCCEEEHHHHHHHHHHH
RRSSASIE
HHHCCCCC
>Mature Secondary Structure 
SQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFIHIGESE
CCCEEEECCCEEEEEECCCCHHHHEEECCCEEECHHHHHHHHHHHCCCCEEEEEECCCC
KNGFIHVSDLGPLRLKKGVVGITELLEPKQKVLVQVMKEPTGNKGPRLTGNISLPGKYLI
CCCEEEEECCCCHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEE
LQPFGQGVNISRKINTDTERSRLRALGVLIKPPSTGLLFRTEAEKIKEELLIEDLENLIQ
EEECCCCCCEEEECCCCHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWDQVLKISETCQPPNLINRDEDFSLKILRDCIKASTSKVVIDNKVALEKAKDFLINNDS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHEECCCC
NVDLVLHNNDINEHILEKYQINKTIQKALEPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVLVIDFIDMESRRDQFQLLEQFTLAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEECC
SARPQIAELTELGLVELTRKRQGQNIYELFGKKCNACNGLGHIKSLPNYQNSNSETNHSA
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCHHH
KKSSRLNNTKFTDLEPSEVIENQEKIVKKELPNSKILNKDDDPAKQKENDNEIVNTHNSK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCEECCCCCC
EKKVITVELSEVEKIVYSQLGINPLIKLGKQYLTNNHLVRLENSNNNEKELSLQKNKKAK
CCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHH
TKVSNKKTNKKSSTSNSIEKSEVEVNESKEINHESKLPKITTANEEVEITDEIDNSRRKR
HHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCEEECCCCCEEEHHHHHHHHHHH
RRSSASIE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]