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The map label for this gene is rnhB

Identifier: 123966915

GI number: 123966915

Start: 1479770

End: 1480399

Strand: Direct

Name: rnhB

Synonym: P9515_16821

Alternate gene names: 123966915

Gene position: 1479770-1480399 (Clockwise)

Preceding gene: 123966914

Following gene: 123966917

Centisome position: 86.83

GC content: 31.75

Gene sequence:

>630_bases
ATGAAATTGATAATTCAAGACGGAAAAGAAGAAGATCATCTGCAAGTATTGAATAAACAACTTGAGATAGGTATTGACGA
AGTAGGTCGAGGTGCAGTTTTTGGACCTGTATTTTCTGCAGCTGTAGTTCTAACCGAAAAAAATGGATATACTCTCAAGC
AATTGGGTGTAACCGATAGTAAAAAACTAACAGAAAAAAAAAGAAAATTACTTTTGCCGAAAATTATCTCATTATCCTCA
GATTACAGTTTGGGACAGTCTTCAGCAAGAGAAATAGATCAACTTGGTATAAGATATGCCACCGAGCTTTCAATGATTAG
AGCTATAAAGAAGTTAAAATCCAAACCATATGAAGTTATAATTGACGGTCCATTATCATTAAGACTTTGGAAGAGTCATC
AAAGAAATATAATTGCAGGTGATTCCAAATTCACATCAATTGCTGCAGCAAGCATAGTTGCAAAAGTAACACGTGACTTT
TTAATGGAAAGATTAGAAAAAAAATTCTCTGGATATTTTATCTTTAAAAATAAAGGCTATGGTACTAAAGAGCATTTATT
AAGTCTTAAAGCTAATGGCGTAACGAATCTACATAGGAAAAGTTTTTTAACTAAATTAAACCTTATTTAA

Upstream 100 bases:

>100_bases
ATAGAAAAGTCTGAAGTTGAAGTAAATGAGTCTAAAGAAATTAATCATGAGAGCAAATTACCAAAGATAACAACTGCTAA
TGAGGAAGTAGAAATAACAG

Downstream 100 bases:

>100_bases
ATCACACCAATTTACAAAATCTTTTATTAATTGTTGCTGAACTCTAGATTTTATTCCTAATAAAATTCCATTCAATACTG
AATGGCCAGTAGATTCTAAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKLIIQDGKEEDHLQVLNKQLEIGIDEVGRGAVFGPVFSAAVVLTEKNGYTLKQLGVTDSKKLTEKKRKLLLPKIISLSS
DYSLGQSSAREIDQLGIRYATELSMIRAIKKLKSKPYEVIIDGPLSLRLWKSHQRNIIAGDSKFTSIAAASIVAKVTRDF
LMERLEKKFSGYFIFKNKGYGTKEHLLSLKANGVTNLHRKSFLTKLNLI

Sequences:

>Translated_209_residues
MKLIIQDGKEEDHLQVLNKQLEIGIDEVGRGAVFGPVFSAAVVLTEKNGYTLKQLGVTDSKKLTEKKRKLLLPKIISLSS
DYSLGQSSAREIDQLGIRYATELSMIRAIKKLKSKPYEVIIDGPLSLRLWKSHQRNIIAGDSKFTSIAAASIVAKVTRDF
LMERLEKKFSGYFIFKNKGYGTKEHLLSLKANGVTNLHRKSFLTKLNLI
>Mature_209_residues
MKLIIQDGKEEDHLQVLNKQLEIGIDEVGRGAVFGPVFSAAVVLTEKNGYTLKQLGVTDSKKLTEKKRKLLLPKIISLSS
DYSLGQSSAREIDQLGIRYATELSMIRAIKKLKSKPYEVIIDGPLSLRLWKSHQRNIIAGDSKFTSIAAASIVAKVTRDF
LMERLEKKFSGYFIFKNKGYGTKEHLLSLKANGVTNLHRKSFLTKLNLI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=42.1621621621622, Blast_Score=129, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PROM5 (A2BYM8)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011996.1
- ProteinModelPortal:   A2BYM8
- SMR:   A2BYM8
- STRING:   A2BYM8
- GeneID:   4720191
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_16821
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK13925
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23450; Mature: 23450

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIQDGKEEDHLQVLNKQLEIGIDEVGRGAVFGPVFSAAVVLTEKNGYTLKQLGVTDS
CEEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCHHHHHHEEEEEECCCCCEEEECCCCCH
KKLTEKKRKLLLPKIISLSSDYSLGQSSAREIDQLGIRYATELSMIRAIKKLKSKPYEVI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDGPLSLRLWKSHQRNIIAGDSKFTSIAAASIVAKVTRDFLMERLEKKFSGYFIFKNKGY
ECCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
GTKEHLLSLKANGVTNLHRKSFLTKLNLI
CHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLIIQDGKEEDHLQVLNKQLEIGIDEVGRGAVFGPVFSAAVVLTEKNGYTLKQLGVTDS
CEEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCHHHHHHEEEEEECCCCCEEEECCCCCH
KKLTEKKRKLLLPKIISLSSDYSLGQSSAREIDQLGIRYATELSMIRAIKKLKSKPYEVI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDGPLSLRLWKSHQRNIIAGDSKFTSIAAASIVAKVTRDFLMERLEKKFSGYFIFKNKGY
ECCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
GTKEHLLSLKANGVTNLHRKSFLTKLNLI
CHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA