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The map label for this gene is nusA [H]

Identifier: 123966905

GI number: 123966905

Start: 1466055

End: 1467458

Strand: Direct

Name: nusA [H]

Synonym: P9515_16721

Alternate gene names: 123966905

Gene position: 1466055-1467458 (Clockwise)

Preceding gene: 123966904

Following gene: 123966906

Centisome position: 86.03

GC content: 38.32

Gene sequence:

>1404_bases
ATGGCATTAGTAATTCTCCCAGGATTGAACAATCTAATTGAAGACATTAGTGAGGAAAAAAAATTACCTCCTCACGTTGT
GGAATCAGCTTTGCGTGAAGCCTTACTTAAAGGTTACGAAAAATACAGAAGAACCTTCTACATTGGAGTAAAAGAAGATC
CATTTGATGAAGAGTATTTTAGTAATTTTGATGTTGGATTTGATTTAGATGAAGAAGGTTATCGAATTTTATCAAGTAAA
ATAATTGTAGAAGAGGTTGAAAGCGAAGATCATCAGATATCTCTTAATGAAGTAAAACAAGTCGCGGACGATGCTCAAAT
AGGTGATACTGTCGTTTTAGATGTTACTCCCGAAAAGGAAGATTTTGGCAGAATGGCTGCCTCAACCACGAAGCAAGTAT
TAGCTCAAAAGTTGAGAGATCAGCAAAGAAAGATGATTCAAGAAGAATTCGCAGATTTAGAGGATCCCGTTTTAACAGCA
AGAGTTATAAGATTTGAAAGACAATCAGTAATTATGGGTGTCAGCTCAGGAATTGGGAGACCAGAAGTAGAAGCAGAACT
GCCTAAAAGGGATCAATTGCCTAATGATAACTATAGAGCTAACGCAACATTCAAAGTATTTCTTAAAGAGGTTAGTGAAA
TTGCGAGAAAAGGGCCTCAACTTTTTGTAAGCAGAGCCAATGCTGGTTTAGTAGTTTATTTATTTGAAAATGAGGTCCCA
GAAATTCAGGAAGGTACAGTTAAAATTGTAGCTGTATCGCGAGAAGCAAACCCTCCTTCAAGAGCTGTCGGCCCAAGAAC
AAAAGTTGCAGTTGATAGTATTGAAAGAGAAGTTGATCCCGTCGGTGCTTGCATTGGAGCAAGAGGAGCAAGAATTCAAC
AAGTCGTTAATGAATTAAGAGGGGAGAAAATTGATGTTATTAAGTGGTCAGCTGATCCTATCGAATATATATTAAATTCT
TTGAGTCCAGCTAAAGTAGACCTTGTTAGACTCGTAGACCCAGAAGGACAACATGCTCATGTTTTAGTTCCTCCTGATCA
ATTAAGTCTTGCGATAGGAAGAGAAGGTCAAAATGTGCGTCTTGCAGCAAGATTAACTGGTTGGAAAATAGACGTTAAAA
ACTCCCATGAATATAATCAAGAAGCGGAAGACGCTGCGGTTGCAGAATTAATTGTTCAAAGAGAGGAAGAGGAGCATCTA
CAAAGAGAAGCTGAACAGCGATTGGAGGCAGAGCAAGCAGAAAGAGCAGCAGAAGATGCAAGATTGAGGGAACTTTATCC
TCTACCTGAAGATGATGAAGAATATGGGAGTGAATCATACGAAGAAGAAAATCTTTCCGAAAATAGCCAATTGGAAAATA
TGAACCCTGATGAAATAATATCTACAGAGGAGAAAGCACGGTGA

Upstream 100 bases:

>100_bases
AAGATAAAAAAAATCCCCTTTAATGAAGTATTAAAGATTAGTCTTTGCACTAGACAAGACTAATAAACTTTTCAACCATT
ATTCATTTTACCCATCATAG

Downstream 100 bases:

>100_bases
CACAAAAAACTCCTGTGATGCGTAAGTGTATTTCCTGCAGGACAACTTATGATAGAAATAATCTGTTGAGGATTACTAAA
GATCATAAATTAGGAATAAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MALVILPGLNNLIEDISEEKKLPPHVVESALREALLKGYEKYRRTFYIGVKEDPFDEEYFSNFDVGFDLDEEGYRILSSK
IIVEEVESEDHQISLNEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIKWSADPIEYILNS
LSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYNQEAEDAAVAELIVQREEEEHL
QREAEQRLEAEQAERAAEDARLRELYPLPEDDEEYGSESYEEENLSENSQLENMNPDEIISTEEKAR

Sequences:

>Translated_467_residues
MALVILPGLNNLIEDISEEKKLPPHVVESALREALLKGYEKYRRTFYIGVKEDPFDEEYFSNFDVGFDLDEEGYRILSSK
IIVEEVESEDHQISLNEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIKWSADPIEYILNS
LSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYNQEAEDAAVAELIVQREEEEHL
QREAEQRLEAEQAERAAEDARLRELYPLPEDDEEYGSESYEEENLSENSQLENMNPDEIISTEEKAR
>Mature_466_residues
ALVILPGLNNLIEDISEEKKLPPHVVESALREALLKGYEKYRRTFYIGVKEDPFDEEYFSNFDVGFDLDEEGYRILSSKI
IVEEVESEDHQISLNEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTAR
VIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVPE
IQEGTVKIVAVSREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIKWSADPIEYILNSL
SPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYNQEAEDAAVAELIVQREEEEHLQ
REAEQRLEAEQAERAAEDARLRELYPLPEDDEEYGSESYEEENLSENSQLENMNPDEIISTEEKAR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=363, Percent_Identity=32.7823691460055, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52711; Mature: 52579

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVILPGLNNLIEDISEEKKLPPHVVESALREALLKGYEKYRRTFYIGVKEDPFDEEYF
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGFDLDEEGYRILSSKIIVEEVESEDHQISLNEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSADPIEYILNSLSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYNQEAEDAAVAELIVQREEEEHLQREAEQRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLRELYPLPEDDEEYGSESYEEENLSENSQLENMNPDEIISTEEKAR
HHHHHCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCHHHHCCCHHCCC
>Mature Secondary Structure 
ALVILPGLNNLIEDISEEKKLPPHVVESALREALLKGYEKYRRTFYIGVKEDPFDEEYF
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGFDLDEEGYRILSSKIIVEEVESEDHQISLNEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSADPIEYILNSLSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYNQEAEDAAVAELIVQREEEEHLQREAEQRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLRELYPLPEDDEEYGSESYEEENLSENSQLENMNPDEIISTEEKAR
HHHHHCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]