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The map label for this gene is murD

Identifier: 123966762

GI number: 123966762

Start: 1357862

End: 1359295

Strand: Direct

Name: murD

Synonym: P9515_15291

Alternate gene names: 123966762

Gene position: 1357862-1359295 (Clockwise)

Preceding gene: 123966757

Following gene: 123966763

Centisome position: 79.68

GC content: 30.26

Gene sequence:

>1434_bases
ATGCCTATTAAAAATAATTCAAAAAACGAAAAAAAAATTTATTTTGTAATTGGGTTAGGTAGATCTGGCTTTTGGGCAGC
AAAGTATTTAAGAAGTCTGGGAAAGAAAGTTATAGTTTGGGAAAGTAATGAAAACGAAGAACTCTCAGAAACAAAAGAGA
TACTAGAGAAACTCGACATATCGGTTTGTCTAAATAAACAATTTTTATTTGAAGAGTTTTCTGAGTGTCTTAATCAGATT
GAAGCTGTTGTTATAAGTCCAGCTATACCCATCGACCATAAAACAATAATCAAACTTAAAGAAAAAGGAATCGAGGTATT
AGGAGAAGTCAATATCGCATGGGAAAGTTTAAAGAATATAAATTGGATTGGAATTACAGGTACCAATGGAAAAACCACAG
TCACACACTTATTAAGTCATATACTTAGAACGAATAATTTATTAGCTCCATTTGCTGGCAATATTGGGACCCCATTATGC
AAAATTGCGTACTCAATTAAAAGCAAAAATATTGATTGGTTAGTAGCTGAATTAAGCAGTTATCAAATAGAAATAGCGAC
ACTTTGCATAAAACCAAAAATTGGAATTTGGACAACATTTACTGCCGACCATCTGGACCGTCACAAAACCCTTGATAATT
ATTTCAAGATAAAAAATAGCTTACTAAAACAATCCGAATTTAGAATTTATAATTATGACGATATACATTTAAGAGAAAAT
TTCAAATCTCTTTCAAAGGGGATTTGGATCACCACCAGTTCTAAACAATCAGATTTAGATCATTGTGATTACTGGATAAA
TAATGAAGACTTTATAGTAGAAAGACAGGAAAATTTATTGAGTTTAAAAAACTTTAAATTAAAAGGTAATCATAATACTC
AAAATCTATTACTAGCCATAGCAGCAGCAAGAAAAATTGGATTATCACCTGAAAAAATAAAGAATGCTTTACTTAGTTAT
GAACAACTTCCTCATAGAATGGAGACGATCTATCAAAGCAATAAGCTTGAAATAATCAATGATAGCAAGGCTACCAATTT
TGACTCTTCTACAGCAGGGATAAAGGCAATTAAAGGTGCTCCCATAATAATTTCCGGGGGAAAGTTAAAAAGTGGCAGTT
ATATTGAGTGGGTAAATATTATTAATAAAAAAGCAAAAGCCGTATTTCTTTTTGGCGAAAGCTCCCAAACTTTAAAAAAA
TTAATCCTTGAGGGAGGATTTAAGAAAGATATTTTCATTTTTAATGATCTTTCAGAGGTAATCAACTATGTCTACTCTTA
TATAGAAAATAATCAAATAGAAACTTTATTGTTTTCTCCGTCATGTTCAAGTTTTGATCAATTTAGAAACTATGAAGAAA
GAGGAGATATTTTTAAAAAATTAATCCATGAAAAATTCAATCTCAAACTTTTCGCGCACAAAGAATTTAGTTAA

Upstream 100 bases:

>100_bases
ATTTATTAATTTTAATTTAGAAATGATATTAGTTTTTAAAGAATTTTTCATAATTAAATAGAACAATTACTTTAGAAACC
TCAATTTAATTATAAATTTA

Downstream 100 bases:

>100_bases
AAGAATTGCTAGGTCGGTCATTACAACCAATTCACCACTAGCAAATATTGTCTTATTTAGTTAGTTTTAAACAAGAAATA
GTAAGAAACATGAGTGAATC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPIKNNSKNEKKIYFVIGLGRSGFWAAKYLRSLGKKVIVWESNENEELSETKEILEKLDISVCLNKQFLFEEFSECLNQI
EAVVISPAIPIDHKTIIKLKEKGIEVLGEVNIAWESLKNINWIGITGTNGKTTVTHLLSHILRTNNLLAPFAGNIGTPLC
KIAYSIKSKNIDWLVAELSSYQIEIATLCIKPKIGIWTTFTADHLDRHKTLDNYFKIKNSLLKQSEFRIYNYDDIHLREN
FKSLSKGIWITTSSKQSDLDHCDYWINNEDFIVERQENLLSLKNFKLKGNHNTQNLLLAIAAARKIGLSPEKIKNALLSY
EQLPHRMETIYQSNKLEIINDSKATNFDSSTAGIKAIKGAPIIISGGKLKSGSYIEWVNIINKKAKAVFLFGESSQTLKK
LILEGGFKKDIFIFNDLSEVINYVYSYIENNQIETLLFSPSCSSFDQFRNYEERGDIFKKLIHEKFNLKLFAHKEFS

Sequences:

>Translated_477_residues
MPIKNNSKNEKKIYFVIGLGRSGFWAAKYLRSLGKKVIVWESNENEELSETKEILEKLDISVCLNKQFLFEEFSECLNQI
EAVVISPAIPIDHKTIIKLKEKGIEVLGEVNIAWESLKNINWIGITGTNGKTTVTHLLSHILRTNNLLAPFAGNIGTPLC
KIAYSIKSKNIDWLVAELSSYQIEIATLCIKPKIGIWTTFTADHLDRHKTLDNYFKIKNSLLKQSEFRIYNYDDIHLREN
FKSLSKGIWITTSSKQSDLDHCDYWINNEDFIVERQENLLSLKNFKLKGNHNTQNLLLAIAAARKIGLSPEKIKNALLSY
EQLPHRMETIYQSNKLEIINDSKATNFDSSTAGIKAIKGAPIIISGGKLKSGSYIEWVNIINKKAKAVFLFGESSQTLKK
LILEGGFKKDIFIFNDLSEVINYVYSYIENNQIETLLFSPSCSSFDQFRNYEERGDIFKKLIHEKFNLKLFAHKEFS
>Mature_476_residues
PIKNNSKNEKKIYFVIGLGRSGFWAAKYLRSLGKKVIVWESNENEELSETKEILEKLDISVCLNKQFLFEEFSECLNQIE
AVVISPAIPIDHKTIIKLKEKGIEVLGEVNIAWESLKNINWIGITGTNGKTTVTHLLSHILRTNNLLAPFAGNIGTPLCK
IAYSIKSKNIDWLVAELSSYQIEIATLCIKPKIGIWTTFTADHLDRHKTLDNYFKIKNSLLKQSEFRIYNYDDIHLRENF
KSLSKGIWITTSSKQSDLDHCDYWINNEDFIVERQENLLSLKNFKLKGNHNTQNLLLAIAAARKIGLSPEKIKNALLSYE
QLPHRMETIYQSNKLEIINDSKATNFDSSTAGIKAIKGAPIIISGGKLKSGSYIEWVNIINKKAKAVFLFGESSQTLKKL
ILEGGFKKDIFIFNDLSEVINYVYSYIENNQIETLLFSPSCSSFDQFRNYEERGDIFKKLIHEKFNLKLFAHKEFS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=469, Percent_Identity=26.865671641791, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PROM5 (A2BY75)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011843.1
- ProteinModelPortal:   A2BY75
- SMR:   A2BY75
- STRING:   A2BY75
- GeneID:   4720600
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_15291
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   LPWVGIT
- ProtClustDB:   PRK02705
- BioCyc:   PMAR167542:P9515ORF_1597-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 54595; Mature: 54463

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKNNSKNEKKIYFVIGLGRSGFWAAKYLRSLGKKVIVWESNENEELSETKEILEKLDI
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCH
SVCLNKQFLFEEFSECLNQIEAVVISPAIPIDHKTIIKLKEKGIEVLGEVNIAWESLKNI
HHEECHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHCCC
NWIGITGTNGKTTVTHLLSHILRTNNLLAPFAGNIGTPLCKIAYSIKSKNIDWLVAELSS
EEEEEECCCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
YQIEIATLCIKPKIGIWTTFTADHLDRHKTLDNYFKIKNSLLKQSEFRIYNYDDIHLREN
CEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
FKSLSKGIWITTSSKQSDLDHCDYWINNEDFIVERQENLLSLKNFKLKGNHNTQNLLLAI
HHHHHCCEEEEECCCCCCCCCCCEEECCCCEEEECHHHHHHHHCEEEECCCCHHHHHHHH
AAARKIGLSPEKIKNALLSYEQLPHRMETIYQSNKLEIINDSKATNFDSSTAGIKAIKGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCC
PIIISGGKLKSGSYIEWVNIINKKAKAVFLFGESSQTLKKLILEGGFKKDIFIFNDLSEV
EEEEECCEECCCCEEHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHH
INYVYSYIENNQIETLLFSPSCSSFDQFRNYEERGDIFKKLIHEKFNLKLFAHKEFS
HHHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCC
>Mature Secondary Structure 
PIKNNSKNEKKIYFVIGLGRSGFWAAKYLRSLGKKVIVWESNENEELSETKEILEKLDI
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCH
SVCLNKQFLFEEFSECLNQIEAVVISPAIPIDHKTIIKLKEKGIEVLGEVNIAWESLKNI
HHEECHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHCCC
NWIGITGTNGKTTVTHLLSHILRTNNLLAPFAGNIGTPLCKIAYSIKSKNIDWLVAELSS
EEEEEECCCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
YQIEIATLCIKPKIGIWTTFTADHLDRHKTLDNYFKIKNSLLKQSEFRIYNYDDIHLREN
CEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
FKSLSKGIWITTSSKQSDLDHCDYWINNEDFIVERQENLLSLKNFKLKGNHNTQNLLLAI
HHHHHCCEEEEECCCCCCCCCCCEEECCCCEEEECHHHHHHHHCEEEECCCCHHHHHHHH
AAARKIGLSPEKIKNALLSYEQLPHRMETIYQSNKLEIINDSKATNFDSSTAGIKAIKGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCC
PIIISGGKLKSGSYIEWVNIINKKAKAVFLFGESSQTLKKLILEGGFKKDIFIFNDLSEV
EEEEECCEECCCCEEHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHH
INYVYSYIENNQIETLLFSPSCSSFDQFRNYEERGDIFKKLIHEKFNLKLFAHKEFS
HHHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA