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The map label for this gene is ligA [H]

Identifier: 123966756

GI number: 123966756

Start: 1355171

End: 1355920

Strand: Direct

Name: ligA [H]

Synonym: P9515_15231

Alternate gene names: 123966756

Gene position: 1355171-1355920 (Clockwise)

Preceding gene: 123966753

Following gene: 123966757

Centisome position: 79.52

GC content: 30.8

Gene sequence:

>750_bases
ATGGGTTCGTTATTAGAAAAAAATGTTAAACATCTTGATGAACAATATAGAATAGGCAACGCTCTAATATCAGATAAAGC
TTTTGATCAACTTGAAAAAAACTTACTCCGAACAGATCCACAATGTGATTACTTTAATCAAAAAAATAATTTATTACTTC
CCTCATTGGCTAATGAAAACCATATAGAGTTTTTAGCGAGCTTGTTAAAAAACACAAGATTGAGCATTCAACCAAAAATT
GATGGATGTGCTATTGCAATTAATTATATAAATGGCAAGTTTAATAAAGCCATTACAAGAACAGGATTTGATGTCACAAG
CAAAATTAAAAAGATTAAAGATGTCCCCAGTCGCCTTCCTATCCAACGAGATTTTCAAGTTAGAGGTGAGCTTTACTCCC
CAAACCAAACTCCCTATTTTTCCCAAAAAATTACAAGCGAATTCCTCAACAATAAAAAAAGGATTGCAAAAAGTTTCAGC
TTTTGCTGTTTCCAAATACTTAATGGAAGACTTAACCAGTATGAAACCCTTAACTATCTTAAAAAATGTGGCTTTAACAC
CCCTCACAGTTACTTCACTAATTTTACAAGCCAAGTCGAGTTATTTAGAAAAAGATGGTTAGATGGAAAAATTTTTTCAA
AATACCCAACTGATGGAATTGTCATCAAAATAAATAGTAGAAAATTGCAGTTGCTTAGAGAAACAAATCTTTCAAAATAT
AATGAATGGCAATATGCAATTAAAAAATAA

Upstream 100 bases:

>100_bases
TGAATTTTTACGGAATCATAAAGTTAAATTTTTATTTATGAAAACTTAACTTTAATAAAAATTCTCCTCAAAAATATTTA
TAAATTATTCAAGAAATATT

Downstream 100 bases:

>100_bases
TTTAATTTCGAAAATAATTAATAAATTAATTAAATCCTAAAAAAATTTTCAAATTAGAAAAAGGAGTAACTTTTTAAGTG
GTCACGATCCTTCTGATTAG

Product: NAD-dependent DNA ligase N-terminus

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MGSLLEKNVKHLDEQYRIGNALISDKAFDQLEKNLLRTDPQCDYFNQKNNLLLPSLANENHIEFLASLLKNTRLSIQPKI
DGCAIAINYINGKFNKAITRTGFDVTSKIKKIKDVPSRLPIQRDFQVRGELYSPNQTPYFSQKITSEFLNNKKRIAKSFS
FCCFQILNGRLNQYETLNYLKKCGFNTPHSYFTNFTSQVELFRKRWLDGKIFSKYPTDGIVIKINSRKLQLLRETNLSKY
NEWQYAIKK

Sequences:

>Translated_249_residues
MGSLLEKNVKHLDEQYRIGNALISDKAFDQLEKNLLRTDPQCDYFNQKNNLLLPSLANENHIEFLASLLKNTRLSIQPKI
DGCAIAINYINGKFNKAITRTGFDVTSKIKKIKDVPSRLPIQRDFQVRGELYSPNQTPYFSQKITSEFLNNKKRIAKSFS
FCCFQILNGRLNQYETLNYLKKCGFNTPHSYFTNFTSQVELFRKRWLDGKIFSKYPTDGIVIKINSRKLQLLRETNLSKY
NEWQYAIKK
>Mature_248_residues
GSLLEKNVKHLDEQYRIGNALISDKAFDQLEKNLLRTDPQCDYFNQKNNLLLPSLANENHIEFLASLLKNTRLSIQPKID
GCAIAINYINGKFNKAITRTGFDVTSKIKKIKDVPSRLPIQRDFQVRGELYSPNQTPYFSQKITSEFLNNKKRIAKSFSF
CCFQILNGRLNQYETLNYLKKCGFNTPHSYFTNFTSQVELFRKRWLDGKIFSKYPTDGIVIKINSRKLQLLRETNLSKYN
EWQYAIKK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 29100; Mature: 28969

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLLEKNVKHLDEQYRIGNALISDKAFDQLEKNLLRTDPQCDYFNQKNNLLLPSLANEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCC
HIEFLASLLKNTRLSIQPKIDGCAIAINYINGKFNKAITRTGFDVTSKIKKIKDVPSRLP
HHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
IQRDFQVRGELYSPNQTPYFSQKITSEFLNNKKRIAKSFSFCCFQILNGRLNQYETLNYL
CCCCHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKCGFNTPHSYFTNFTSQVELFRKRWLDGKIFSKYPTDGIVIKINSRKLQLLRETNLSKY
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCHHHHHHHHCCCCHH
NEWQYAIKK
HHHHHHCCC
>Mature Secondary Structure 
GSLLEKNVKHLDEQYRIGNALISDKAFDQLEKNLLRTDPQCDYFNQKNNLLLPSLANEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCC
HIEFLASLLKNTRLSIQPKIDGCAIAINYINGKFNKAITRTGFDVTSKIKKIKDVPSRLP
HHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
IQRDFQVRGELYSPNQTPYFSQKITSEFLNNKKRIAKSFSFCCFQILNGRLNQYETLNYL
CCCCHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKCGFNTPHSYFTNFTSQVELFRKRWLDGKIFSKYPTDGIVIKINSRKLQLLRETNLSKY
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCHHHHHHHHCCCCHH
NEWQYAIKK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA