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The map label for this gene is tyrS [H]

Identifier: 123966723

GI number: 123966723

Start: 1324925

End: 1326163

Strand: Reverse

Name: tyrS [H]

Synonym: P9515_14901

Alternate gene names: 123966723

Gene position: 1326163-1324925 (Counterclockwise)

Preceding gene: 123966724

Following gene: 123966722

Centisome position: 77.82

GC content: 30.19

Gene sequence:

>1239_bases
ATGGTAGATAAATTTGAATTGCCAAAATGGCTCACGAGGGGAATAGAAGAATCATTCCCACTCAAAAACTTTGATCAAAC
ACTTGCATATAAAATTGATGAGGCAAATAAAAATAAAAAAAAATTAAGAGTAAAACTTGGTATTGATCCAACTGGAACTG
ATATTCATCTTGGTCATAGCATTTTATTCAGAAAACTTAGAGCATTCCAAGATAATGGTCATGTAGCGGTACTAATAATT
GGAGATTTTACTGCACAAATAGGAGATCCAACAGGAAAAAACAAAACAAGAGTTCAATTATCCGAAGAAGAGGTTAAAGA
AAACTCAAAAACTTATTTGAATCAACTTGGTATGGGTAAATCTTCTGATAAATCTATTTTGGATTTTGAATCAGAAGATA
GGATAGAAATTAGATACAATAGCGAATGGTTAAAAAATCTAGACCTTAATTCAATTATTGAACTAATGGGAAGATCTACA
GTTAGCCAAATGCTTGCTAAGGAAGAATTTAATAAAAGATATAACGATCAAACCCCAATTGCATTACATGAATTTCTATA
CCCATTACTACAAGGCTATGATTCAGTGATCGTGAATTCGGATATTGAACTAGGTGGAACTGATCAGAAATTTAATATTG
CCATAGGAAGGGATTTGCAAAGGCACTTTAAAAAAGAACCTCAATATGGAATATTGCTACCGATTTTAACTGGCTTAGAT
GGAATTAAAAAAATGAGTAAATCAGAATTCAATACTGTTGGCTTGAGCGAGGATTCACTATCTATGTATTCAAAATTAGA
AAAAGTACCTGATCAACTTATTCCATCATATTTCGAATTGCTTACAGAATTAGATTTAAGTTTCATTAAAGAAACCAATC
CTCGAGATTTACAAAGACTTATGGCTTTGGAAGTTACATCTTTGTACCATGGGAAGAAAGAAGCATTAAAAGCACAATCT
AATTGTGAAAAAATTTTTCTAGGCCTTAAAGAAAAAGTTGGAGATATTCCAATAATTTCTTTAAAAGAGATAGTATTTCC
TGTAAAGCTTTTTTATTTGCTTAGCTCATTAAATTTATTTAAATCAAGCAGTGAATCAAAAAGATCTATTAAAGGTGGTG
GTGTGAAAATTGATAGTAATAAATTAGTAAATCCAGACCTTGTTTTTGATTCGAAAGAAGCTTTAAAAGGTAAAATTTTG
CAAATTGGCAAAAAAATAATTAAAAGATTTGAGAATTGA

Upstream 100 bases:

>100_bases
TATTACTCCTGATCAAATGTTTGATCAAATGAACAAAACATTGATAAGAATGAAGGAAGAGCTTGATAAGTCTTCTTAAC
TTCCTAAAATTGAAATTTTC

Downstream 100 bases:

>100_bases
TTTTAAATGAACAAAATTAATTTAGAAGATAAAATAATATTAGCTATTGACGGATTAAATATTCAAGAAGCAAAATTATT
TCTTGAGAGATGTCCCAATA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MVDKFELPKWLTRGIEESFPLKNFDQTLAYKIDEANKNKKKLRVKLGIDPTGTDIHLGHSILFRKLRAFQDNGHVAVLII
GDFTAQIGDPTGKNKTRVQLSEEEVKENSKTYLNQLGMGKSSDKSILDFESEDRIEIRYNSEWLKNLDLNSIIELMGRST
VSQMLAKEEFNKRYNDQTPIALHEFLYPLLQGYDSVIVNSDIELGGTDQKFNIAIGRDLQRHFKKEPQYGILLPILTGLD
GIKKMSKSEFNTVGLSEDSLSMYSKLEKVPDQLIPSYFELLTELDLSFIKETNPRDLQRLMALEVTSLYHGKKEALKAQS
NCEKIFLGLKEKVGDIPIISLKEIVFPVKLFYLLSSLNLFKSSSESKRSIKGGGVKIDSNKLVNPDLVFDSKEALKGKIL
QIGKKIIKRFEN

Sequences:

>Translated_412_residues
MVDKFELPKWLTRGIEESFPLKNFDQTLAYKIDEANKNKKKLRVKLGIDPTGTDIHLGHSILFRKLRAFQDNGHVAVLII
GDFTAQIGDPTGKNKTRVQLSEEEVKENSKTYLNQLGMGKSSDKSILDFESEDRIEIRYNSEWLKNLDLNSIIELMGRST
VSQMLAKEEFNKRYNDQTPIALHEFLYPLLQGYDSVIVNSDIELGGTDQKFNIAIGRDLQRHFKKEPQYGILLPILTGLD
GIKKMSKSEFNTVGLSEDSLSMYSKLEKVPDQLIPSYFELLTELDLSFIKETNPRDLQRLMALEVTSLYHGKKEALKAQS
NCEKIFLGLKEKVGDIPIISLKEIVFPVKLFYLLSSLNLFKSSSESKRSIKGGGVKIDSNKLVNPDLVFDSKEALKGKIL
QIGKKIIKRFEN
>Mature_412_residues
MVDKFELPKWLTRGIEESFPLKNFDQTLAYKIDEANKNKKKLRVKLGIDPTGTDIHLGHSILFRKLRAFQDNGHVAVLII
GDFTAQIGDPTGKNKTRVQLSEEEVKENSKTYLNQLGMGKSSDKSILDFESEDRIEIRYNSEWLKNLDLNSIIELMGRST
VSQMLAKEEFNKRYNDQTPIALHEFLYPLLQGYDSVIVNSDIELGGTDQKFNIAIGRDLQRHFKKEPQYGILLPILTGLD
GIKKMSKSEFNTVGLSEDSLSMYSKLEKVPDQLIPSYFELLTELDLSFIKETNPRDLQRLMALEVTSLYHGKKEALKAQS
NCEKIFLGLKEKVGDIPIISLKEIVFPVKLFYLLSSLNLFKSSSESKRSIKGGGVKIDSNKLVNPDLVFDSKEALKGKIL
QIGKKIIKRFEN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 S4 RNA-binding domains [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=436, Percent_Identity=27.5229357798165, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1787925, Length=388, Percent_Identity=28.0927835051546, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=343, Percent_Identity=25.9475218658892, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=302, Percent_Identity=28.1456953642384, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20130383, Length=395, Percent_Identity=24.8101265822785, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46857; Mature: 46857

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKFELPKWLTRGIEESFPLKNFDQTLAYKIDEANKNKKKLRVKLGIDPTGTDIHLGHS
CCCCCCCCHHHHHCHHHCCCCCCCCHHHHEEECCCCCCCEEEEEEECCCCCCCCEECCHH
ILFRKLRAFQDNGHVAVLIIGDFTAQIGDPTGKNKTRVQLSEEEVKENSKTYLNQLGMGK
HHHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC
SSDKSILDFESEDRIEIRYNSEWLKNLDLNSIIELMGRSTVSQMLAKEEFNKRYNDQTPI
CCCCCEECCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ALHEFLYPLLQGYDSVIVNSDIELGGTDQKFNIAIGRDLQRHFKKEPQYGILLPILTGLD
HHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEHHHHHCCH
GIKKMSKSEFNTVGLSEDSLSMYSKLEKVPDQLIPSYFELLTELDLSFIKETNPRDLQRL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MALEVTSLYHGKKEALKAQSNCEKIFLGLKEKVGDIPIISLKEIVFPVKLFYLLSSLNLF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
KSSSESKRSIKGGGVKIDSNKLVNPDLVFDSKEALKGKILQIGKKIIKRFEN
HCCCHHHHHCCCCCEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVDKFELPKWLTRGIEESFPLKNFDQTLAYKIDEANKNKKKLRVKLGIDPTGTDIHLGHS
CCCCCCCCHHHHHCHHHCCCCCCCCHHHHEEECCCCCCCEEEEEEECCCCCCCCEECCHH
ILFRKLRAFQDNGHVAVLIIGDFTAQIGDPTGKNKTRVQLSEEEVKENSKTYLNQLGMGK
HHHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC
SSDKSILDFESEDRIEIRYNSEWLKNLDLNSIIELMGRSTVSQMLAKEEFNKRYNDQTPI
CCCCCEECCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ALHEFLYPLLQGYDSVIVNSDIELGGTDQKFNIAIGRDLQRHFKKEPQYGILLPILTGLD
HHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEHHHHHCCH
GIKKMSKSEFNTVGLSEDSLSMYSKLEKVPDQLIPSYFELLTELDLSFIKETNPRDLQRL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MALEVTSLYHGKKEALKAQSNCEKIFLGLKEKVGDIPIISLKEIVFPVKLFYLLSSLNLF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
KSSSESKRSIKGGGVKIDSNKLVNPDLVFDSKEALKGKILQIGKKIIKRFEN
HCCCHHHHHCCCCCEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]