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The map label for this gene is pyrF

Identifier: 123966722

GI number: 123966722

Start: 1324199

End: 1324918

Strand: Reverse

Name: pyrF

Synonym: P9515_14891

Alternate gene names: 123966722

Gene position: 1324918-1324199 (Counterclockwise)

Preceding gene: 123966723

Following gene: 123966715

Centisome position: 77.75

GC content: 30.0

Gene sequence:

>720_bases
ATGAACAAAATTAATTTAGAAGATAAAATAATATTAGCTATTGACGGATTAAATATTCAAGAAGCAAAATTATTTCTTGA
GAGATGTCCCAATATTAAATGGGTAAAAGTTGGATTAGAACTGTTTACAAGGGAAGGCCCTAATGTAATAAAATTTTTGA
AAGATTTAAATAAAAAAATTTTTTTAGATCTTAAATTTCACGATATTCCAAATACTATGGAGGCTGCTTGTTATGAGGTT
TCCAAATTAGGAGTTGATATTATATCTGTTCACGCTTCTTCAGGTTATAAAGCACTCACCTCATCAAAAAATGCATCTTT
AGATGGAGCAAATTTAGCTAGTGTGAATCCTCCCAATATTGTAGGTATAACTGTTTTAACTAGTTTCTCTAGTGAAGAAT
TTAGAAATGATCTTGATAGGAAGAACTCTATTGAAGAGAATGTTGTTAGGCTCGCAAAATTATGTTTTGATGCAGGATTA
GATGGATGCGTTTGTTCCCCATGGGAGGCAAAAAGATTGAGATCAATTTATAAAAATAATTTCGAGTTGATTACACCTGG
TATAAGAACTAAGACTCAAAATAAAGATGATCAGAATAGAATAATGACTCCCTATGAAGCGTTAAAAAATGGAGCTTCTA
GATTGGTTATTGGAAGAGCTATTTCAAAAGCTAAAGATCCTAATAAGGTTTTTTTAGATATCTGTAATTCTATTTATTAA

Upstream 100 bases:

>100_bases
AGTAAATCCAGACCTTGTTTTTGATTCGAAAGAAGCTTTAAAAGGTAAAATTTTGCAAATTGGCAAAAAAATAATTAAAA
GATTTGAGAATTGATTTTAA

Downstream 100 bases:

>100_bases
GGCTATTAATCTTTGATTCTTCTCCTTTAATAAGACGTCTGATATTTGTTCTATGCTTCAAAATAACTAATATTGATACA
ACTAAACTAATTAAGAAGTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNKINLEDKIILAIDGLNIQEAKLFLERCPNIKWVKVGLELFTREGPNVIKFLKDLNKKIFLDLKFHDIPNTMEAACYEV
SKLGVDIISVHASSGYKALTSSKNASLDGANLASVNPPNIVGITVLTSFSSEEFRNDLDRKNSIEENVVRLAKLCFDAGL
DGCVCSPWEAKRLRSIYKNNFELITPGIRTKTQNKDDQNRIMTPYEALKNGASRLVIGRAISKAKDPNKVFLDICNSIY

Sequences:

>Translated_239_residues
MNKINLEDKIILAIDGLNIQEAKLFLERCPNIKWVKVGLELFTREGPNVIKFLKDLNKKIFLDLKFHDIPNTMEAACYEV
SKLGVDIISVHASSGYKALTSSKNASLDGANLASVNPPNIVGITVLTSFSSEEFRNDLDRKNSIEENVVRLAKLCFDAGL
DGCVCSPWEAKRLRSIYKNNFELITPGIRTKTQNKDDQNRIMTPYEALKNGASRLVIGRAISKAKDPNKVFLDICNSIY
>Mature_239_residues
MNKINLEDKIILAIDGLNIQEAKLFLERCPNIKWVKVGLELFTREGPNVIKFLKDLNKKIFLDLKFHDIPNTMEAACYEV
SKLGVDIISVHASSGYKALTSSKNASLDGANLASVNPPNIVGITVLTSFSSEEFRNDLDRKNSIEENVVRLAKLCFDAGL
DGCVCSPWEAKRLRSIYKNNFELITPGIRTKTQNKDDQNRIMTPYEALKNGASRLVIGRAISKAKDPNKVFLDICNSIY

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=37.8260869565217, Blast_Score=153, Evalue=8e-39,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PROM5 (A2BY35)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011803.1
- ProteinModelPortal:   A2BY35
- SMR:   A2BY35
- STRING:   A2BY35
- GeneID:   4720053
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_14891
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK922152
- BioCyc:   PMAR167542:P9515ORF_1556-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26738; Mature: 26738

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 65-65 BINDING 15-15 BINDING 36-36 BINDING 127-127 BINDING 189-189 BINDING 198-198 BINDING 218-218 BINDING 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKINLEDKIILAIDGLNIQEAKLFLERCPNIKWVKVGLELFTREGPNVIKFLKDLNKKI
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHCCCEE
FLDLKFHDIPNTMEAACYEVSKLGVDIISVHASSGYKALTSSKNASLDGANLASVNPPNI
EEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCE
VGITVLTSFSSEEFRNDLDRKNSIEENVVRLAKLCFDAGLDGCVCSPWEAKRLRSIYKNN
EEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCC
FELITPGIRTKTQNKDDQNRIMTPYEALKNGASRLVIGRAISKAKDPNKVFLDICNSIY
CEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCHHEEHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNKINLEDKIILAIDGLNIQEAKLFLERCPNIKWVKVGLELFTREGPNVIKFLKDLNKKI
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHCCCEE
FLDLKFHDIPNTMEAACYEVSKLGVDIISVHASSGYKALTSSKNASLDGANLASVNPPNI
EEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCE
VGITVLTSFSSEEFRNDLDRKNSIEENVVRLAKLCFDAGLDGCVCSPWEAKRLRSIYKNN
EEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCC
FELITPGIRTKTQNKDDQNRIMTPYEALKNGASRLVIGRAISKAKDPNKVFLDICNSIY
CEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCHHEEHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA