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The map label for this gene is trmD [H]

Identifier: 123966674

GI number: 123966674

Start: 1275493

End: 1276716

Strand: Reverse

Name: trmD [H]

Synonym: P9515_14411

Alternate gene names: 123966674

Gene position: 1276716-1275493 (Counterclockwise)

Preceding gene: 123966675

Following gene: 123966673

Centisome position: 74.92

GC content: 32.76

Gene sequence:

>1224_bases
ATGACTAATTTGAATTTTGAAGTAATTACTTTGTTCCCCAAAGCTTTTGAATTAATAAATAATTTAGGTGTAATAACAAG
AGCTTTAGAGAAAAAGTTAATCAAGGTGAACTTACATGATTTAAGAGAATACGGAGAAGGTTCGTATAGACAAGTCGATG
ACAAGCCTTATGGAGGAGGTTCGGGGATGGTTTTAAAACCAGGACCGATTTTTAAGGCTCATGATTCAATAAAAAAATTA
CCTAATATTAAAACTTTATTAATGAGTCCACAAGGTAAAGTTTTAAAGCAGCAGGATCTTTTAAGATGGTCTACTTTAGA
TCAATTAATAATTATATGCGGGCAATATGAAGGTTTTGATGAAAGAGTTAGATGCTTGGCTGATGAAGAAGTTTCTATAG
GTGATTATGTACTTTCAGGAGGGGAAATTCCTGCTATGACTATAATTAATGGTTTGACAAGATTATTACCTGGAACCCTT
GGAGATCCTAGATCTTTAATAGATGAGAGTCATAATTCATCTTTATTAGAATATCCGCATTATACGAGACCTCTAATTTT
TAGAGAAATGAAAGTACCAGATATTCTTGTTAGTGGTAATCATGAAGAGATTGAATTATGGCGTAAACAAAAAATGTTTG
AAAGAACATTAGAGAGAAGAAATGACTTGATTTCGTATGAAGACTATAAAAAATCACCAAAAAGTAAGAGAATAGATGAA
GACTCAAATGAAATGATGAAATTCCGTATAGGAAATGGATATGATATTCACCGATTAGTAAAGGGTAGAAACTTAATCAT
TGGAGGAGTTAAATTGCAACACCCTGATGATTTAGGACTGGATGGTCATAGCGACGCTGACGTTCTTAGCCACTCAATAA
TGGATGCATTGTTAGGAGCTCTATCTCTTGGTGACATCGGAAAATACTTCCCTCCATCTGATCAAAAATGGAAAGATGTA
GATAGTTTATTTTTGTTATCTAAAGTTATCGAATTAATTAGAAATCAAGGTTGGGAAGTAAATAATATTGATAGTGTTAT
TGTGGCAGAAAGACCAAAAATTAAACCTTTTGTGGAATTAATGAAGAATAATATTTCTGATATCTTGCAAATCGAAGATA
GTTTTATTGGAATAAAGGCTACTACAAATGAGAGATTGGGTCCTGAGGGTAGAGAAGAGGGGATTAGTTGTCACTCTGTA
GTATTGCTTGAGAAAAAAGAATGA

Upstream 100 bases:

>100_bases
AAATGGCTCAGTTCATCTGGAATTATTTGTCAAAGTAATACCAAATTGGAGAAAAAAAGAATCTAAATTAATGGAGTTCG
GTTATGAGGTGGATTACTAA

Downstream 100 bases:

>100_bases
AAATAAACTTTAAAATTAAAGAGAAGATAAAAAACCTTACTTTTTTGTTTATTTGTCTCTTTATTTTTCTATCTCAAATC
AATTCTCCAATCCTTAAAGC

Product: putative bifunctional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTNLNFEVITLFPKAFELINNLGVITRALEKKLIKVNLHDLREYGEGSYRQVDDKPYGGGSGMVLKPGPIFKAHDSIKKL
PNIKTLLMSPQGKVLKQQDLLRWSTLDQLIIICGQYEGFDERVRCLADEEVSIGDYVLSGGEIPAMTIINGLTRLLPGTL
GDPRSLIDESHNSSLLEYPHYTRPLIFREMKVPDILVSGNHEEIELWRKQKMFERTLERRNDLISYEDYKKSPKSKRIDE
DSNEMMKFRIGNGYDIHRLVKGRNLIIGGVKLQHPDDLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDQKWKDV
DSLFLLSKVIELIRNQGWEVNNIDSVIVAERPKIKPFVELMKNNISDILQIEDSFIGIKATTNERLGPEGREEGISCHSV
VLLEKKE

Sequences:

>Translated_407_residues
MTNLNFEVITLFPKAFELINNLGVITRALEKKLIKVNLHDLREYGEGSYRQVDDKPYGGGSGMVLKPGPIFKAHDSIKKL
PNIKTLLMSPQGKVLKQQDLLRWSTLDQLIIICGQYEGFDERVRCLADEEVSIGDYVLSGGEIPAMTIINGLTRLLPGTL
GDPRSLIDESHNSSLLEYPHYTRPLIFREMKVPDILVSGNHEEIELWRKQKMFERTLERRNDLISYEDYKKSPKSKRIDE
DSNEMMKFRIGNGYDIHRLVKGRNLIIGGVKLQHPDDLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDQKWKDV
DSLFLLSKVIELIRNQGWEVNNIDSVIVAERPKIKPFVELMKNNISDILQIEDSFIGIKATTNERLGPEGREEGISCHSV
VLLEKKE
>Mature_406_residues
TNLNFEVITLFPKAFELINNLGVITRALEKKLIKVNLHDLREYGEGSYRQVDDKPYGGGSGMVLKPGPIFKAHDSIKKLP
NIKTLLMSPQGKVLKQQDLLRWSTLDQLIIICGQYEGFDERVRCLADEEVSIGDYVLSGGEIPAMTIINGLTRLLPGTLG
DPRSLIDESHNSSLLEYPHYTRPLIFREMKVPDILVSGNHEEIELWRKQKMFERTLERRNDLISYEDYKKSPKSKRIDED
SNEMMKFRIGNGYDIHRLVKGRNLIIGGVKLQHPDDLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDQKWKDVD
SLFLLSKVIELIRNQGWEVNNIDSVIVAERPKIKPFVELMKNNISDILQIEDSFIGIKATTNERLGPEGREEGISCHSVV
LLEKKE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=220, Percent_Identity=42.7272727272727, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=43.6708860759494, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 46133; Mature: 46002

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNFEVITLFPKAFELINNLGVITRALEKKLIKVNLHDLREYGEGSYRQVDDKPYGGG
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCCCCC
SGMVLKPGPIFKAHDSIKKLPNIKTLLMSPQGKVLKQQDLLRWSTLDQLIIICGQYEGFD
CCEEECCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCHH
ERVRCLADEEVSIGDYVLSGGEIPAMTIINGLTRLLPGTLGDPRSLIDESHNSSLLEYPH
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEECCCC
YTRPLIFREMKVPDILVSGNHEEIELWRKQKMFERTLERRNDLISYEDYKKSPKSKRIDE
CCCCHHHECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
DSNEMMKFRIGNGYDIHRLVKGRNLIIGGVKLQHPDDLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDQKWKDVDSLFLLSKVIELIRNQGWEVNNIDSVIVAERPKIKPFVEL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
MKNNISDILQIEDSFIGIKATTNERLGPEGREEGISCHSVVLLEKKE
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEEECC
>Mature Secondary Structure 
TNLNFEVITLFPKAFELINNLGVITRALEKKLIKVNLHDLREYGEGSYRQVDDKPYGGG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCCCCC
SGMVLKPGPIFKAHDSIKKLPNIKTLLMSPQGKVLKQQDLLRWSTLDQLIIICGQYEGFD
CCEEECCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCHH
ERVRCLADEEVSIGDYVLSGGEIPAMTIINGLTRLLPGTLGDPRSLIDESHNSSLLEYPH
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEECCCC
YTRPLIFREMKVPDILVSGNHEEIELWRKQKMFERTLERRNDLISYEDYKKSPKSKRIDE
CCCCHHHECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
DSNEMMKFRIGNGYDIHRLVKGRNLIIGGVKLQHPDDLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDQKWKDVDSLFLLSKVIELIRNQGWEVNNIDSVIVAERPKIKPFVEL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
MKNNISDILQIEDSFIGIKATTNERLGPEGREEGISCHSVVLLEKKE
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA