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The map label for this gene is wcaF [H]

Identifier: 123966609

GI number: 123966609

Start: 1210881

End: 1211432

Strand: Reverse

Name: wcaF [H]

Synonym: P9515_13761

Alternate gene names: 123966609

Gene position: 1211432-1210881 (Counterclockwise)

Preceding gene: 123966610

Following gene: 123966608

Centisome position: 71.09

GC content: 26.99

Gene sequence:

>552_bases
ATGCGACTTCAAAATTTAAATTTATATGAAAAGAAAAAAAATAGTATTTCAATAATAATAGTTAAATTAATTTGGGATTT
TTTTGGCAAACCTCTTTTTTCTAGCTACATTCCTGGAACATATTGGAGGAAGTTAATTTTAAGAATTTTTAGAGCTAAAA
TTGGAAAAGGAGGAAAAATAAAAACAAATATAAAAATTTCTGAACCATGGAATTTATCAATAGGAGATCATTGTTGGATT
GGTGAAGATGTATGGATAGATAATTTAGCTTTAGTTAAAATTGGCAATAGAGTTTGTATTTCTCAAGGAGTATATTTATG
CACTGGAAATCATAACTATAAAAAAGATTTATTTAATTTAATTTTGGAAAGAATTGTTATTGAGGATGATTGCTGGATAG
CTGCAAAATCAATTATTGCCCCAGGAGCTATTTTAAAAAGAGGTTCTGTGACATGTTTAGGTTCTTTAGTCTCAGGTATA
CTAGAAAAAGATGGAATTTATAAAGGTAACCCTGCTAAGCTTTTTAAATTAAAAGATGATAACCTCAATTAA

Upstream 100 bases:

>100_bases
ATTTATATAGATCAGTTTGTGAAGATATCTTATGAAATTGTAAATATTTAAAATAATTAAAGATGTTTAATATAAATTTA
AAATAAGGAAATTATTGATA

Downstream 100 bases:

>100_bases
AATAATTGTTCCAACTTTAAATACATATTTAATTCTACCTAAGTTGATAAATTCATTAAAAATTCAAACTTGGAAAGACT
GGAAATTATTATTTATTGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MRLQNLNLYEKKKNSISIIIVKLIWDFFGKPLFSSYIPGTYWRKLILRIFRAKIGKGGKIKTNIKISEPWNLSIGDHCWI
GEDVWIDNLALVKIGNRVCISQGVYLCTGNHNYKKDLFNLILERIVIEDDCWIAAKSIIAPGAILKRGSVTCLGSLVSGI
LEKDGIYKGNPAKLFKLKDDNLN

Sequences:

>Translated_183_residues
MRLQNLNLYEKKKNSISIIIVKLIWDFFGKPLFSSYIPGTYWRKLILRIFRAKIGKGGKIKTNIKISEPWNLSIGDHCWI
GEDVWIDNLALVKIGNRVCISQGVYLCTGNHNYKKDLFNLILERIVIEDDCWIAAKSIIAPGAILKRGSVTCLGSLVSGI
LEKDGIYKGNPAKLFKLKDDNLN
>Mature_183_residues
MRLQNLNLYEKKKNSISIIIVKLIWDFFGKPLFSSYIPGTYWRKLILRIFRAKIGKGGKIKTNIKISEPWNLSIGDHCWI
GEDVWIDNLALVKIGNRVCISQGVYLCTGNHNYKKDLFNLILERIVIEDDCWIAAKSIIAPGAILKRGSVTCLGSLVSGI
LEKDGIYKGNPAKLFKLKDDNLN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=140, Percent_Identity=37.8571428571429, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 20739; Mature: 20739

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQNLNLYEKKKNSISIIIVKLIWDFFGKPLFSSYIPGTYWRKLILRIFRAKIGKGGKI
CCCCCCCHHHHCCCCEEEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEE
KTNIKISEPWNLSIGDHCWIGEDVWIDNLALVKIGNRVCISQGVYLCTGNHNYKKDLFNL
EEEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCEEEECCCEEEEECCCCHHHHHHHH
ILERIVIEDDCWIAAKSIIAPGAILKRGSVTCLGSLVSGILEKDGIYKGNPAKLFKLKDD
HHHHHHCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
NLN
CCC
>Mature Secondary Structure
MRLQNLNLYEKKKNSISIIIVKLIWDFFGKPLFSSYIPGTYWRKLILRIFRAKIGKGGKI
CCCCCCCHHHHCCCCEEEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEE
KTNIKISEPWNLSIGDHCWIGEDVWIDNLALVKIGNRVCISQGVYLCTGNHNYKKDLFNL
EEEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCEEEECCCEEEEECCCCHHHHHHHH
ILERIVIEDDCWIAAKSIIAPGAILKRGSVTCLGSLVSGILEKDGIYKGNPAKLFKLKDD
HHHHHHCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
NLN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]