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The map label for this gene is gid

Identifier: 123966440

GI number: 123966440

Start: 1064648

End: 1066051

Strand: Reverse

Name: gid

Synonym: P9515_12071

Alternate gene names: 123966440

Gene position: 1066051-1064648 (Counterclockwise)

Preceding gene: 123966441

Following gene: 123966439

Centisome position: 62.56

GC content: 33.33

Gene sequence:

>1404_bases
TTGTTAAATAAAGAAGTTATCGTAATAGGAGCTGGTCTTGCTGGTTGTGAAGCCGCTTGGCAAATAGCTAATTCTGGAGT
AGAAGTGAAACTTGTTGAAATGAGGCCCATTCATTCAACTCCAGCACATCATACGAGTGAATTTGGTGAATTAGTGTGCA
GTAATAGTTTTGGAGCTTTAAGCACTGATAGAGCAGCAGGTTTATTACAGGAAGAATTAAGAACCTTTAATTCTTTGATA
ATAAATACGGCAGATGAATTTTCTGTGCCTGCTGGAGGAGCTTTAGCAGTAGATAGGTCTAAATTCAGTAAGTCATTAAC
GCAAACATTATCGAATCATCCTCTTGTGGAGATAAAGAGGTTTGAGCAACTAGATCTTCCAGATAAAAATAGAATCACTG
TTATTGCAACAGGACCCCTGACTTCTAATGAATTAGCTAATAAATTAAAAAACTTTACTGGTTTAGATTCTTGCCATTTT
TTTGATGCAGCTAGCCCCATTATTTATGGGGAGACAATTAATTACGATATTGCTTTTAGGGCGAGTAGATACGACAAAGG
AGATCCTGCATATTTAAATTGTCCTATGAATAAAATAGATTATTTAAATTTCAGAAATGCTTTAATTGAAGCTGAACAGG
CGACTTTAAAAGATTTTGAAAAAGAGTCTGCAAATTTTTTTGAAGCTTGTTTACCCATAGAAGAGATGGCTAGAAGAGGA
GTTGACACAATGAGATATGGACCTCTGAAATCTATCGGTTTGTGGAATCCGAAATGGGGAGATTTATTTGACAGGGAAAA
TAGATTAAAAAAAAGGCCTCATGCAATTGTTCAATTAAGAAAAGAAGATCTTAAGGGGAAATTACTTAATATGGTGGGTT
TTCAAACTAACCTTAAATGGTCAGAGCAAAAAAGAATATTTAGGATGATTCCTGGATTAGAAAAAGCTGAGTTTGTTCGT
TTTGGAGTGATGCATAGAAATACATTTTTAGAATCTCCTAAATTACTTTTGCCAACACTTCAATTTCTTAAAAGAGAAAA
TCTTTTTGCAGCAGGTCAAATAACAGGAACAGAAGGCTACGCTGCAGCTGCTTCGGGTGGTTTGTTAGCAGGATTAAATG
CCTCATTATTAGCTAATAATAAAAGTCCAGTAACTTTTCCTGACGAATCAATGATTGGAGCTTTAATGAACTTCATAAGT
AATAAAAATGAAATAATGTCTCAGCAGAAAAAAAATAAATTTCAACCAATGCCTGCTTCCTTTGGTTTGGTACCAGAATT
AAATAATAAAATAAAGGATAAAAAACTAAGATATAAAGCATATCAAAAAAGGTCTTTAGAAGTATTAAAAGAATTTAAAA
AAGTATTAGATTCATCTTTTGAAAAAGATCACATACTTGTTTAG

Upstream 100 bases:

>100_bases
TCCCATTATTGCTGCTCTTGCGTGGGTTATTTTTAATATACAAAAACCCGCAAGAGAACAATTTAATCGAGATTTTTTGG
GAAAAAAGGATTAAATTTAA

Downstream 100 bases:

>100_bases
ATAACTTAAAATGAATTATTAAAAAAATTAAAAATGCAATCTTATAAAGAAAGTTTTGATGCAATTATTATTGGCTCTGG
AATAGGGGGATTGGTAACTG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLNKEVIVIGAGLAGCEAAWQIANSGVEVKLVEMRPIHSTPAHHTSEFGELVCSNSFGALSTDRAAGLLQEELRTFNSLI
INTADEFSVPAGGALAVDRSKFSKSLTQTLSNHPLVEIKRFEQLDLPDKNRITVIATGPLTSNELANKLKNFTGLDSCHF
FDAASPIIYGETINYDIAFRASRYDKGDPAYLNCPMNKIDYLNFRNALIEAEQATLKDFEKESANFFEACLPIEEMARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAIVQLRKEDLKGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAASGGLLAGLNASLLANNKSPVTFPDESMIGALMNFIS
NKNEIMSQQKKNKFQPMPASFGLVPELNNKIKDKKLRYKAYQKRSLEVLKEFKKVLDSSFEKDHILV

Sequences:

>Translated_467_residues
MLNKEVIVIGAGLAGCEAAWQIANSGVEVKLVEMRPIHSTPAHHTSEFGELVCSNSFGALSTDRAAGLLQEELRTFNSLI
INTADEFSVPAGGALAVDRSKFSKSLTQTLSNHPLVEIKRFEQLDLPDKNRITVIATGPLTSNELANKLKNFTGLDSCHF
FDAASPIIYGETINYDIAFRASRYDKGDPAYLNCPMNKIDYLNFRNALIEAEQATLKDFEKESANFFEACLPIEEMARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAIVQLRKEDLKGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAASGGLLAGLNASLLANNKSPVTFPDESMIGALMNFIS
NKNEIMSQQKKNKFQPMPASFGLVPELNNKIKDKKLRYKAYQKRSLEVLKEFKKVLDSSFEKDHILV
>Mature_467_residues
MLNKEVIVIGAGLAGCEAAWQIANSGVEVKLVEMRPIHSTPAHHTSEFGELVCSNSFGALSTDRAAGLLQEELRTFNSLI
INTADEFSVPAGGALAVDRSKFSKSLTQTLSNHPLVEIKRFEQLDLPDKNRITVIATGPLTSNELANKLKNFTGLDSCHF
FDAASPIIYGETINYDIAFRASRYDKGDPAYLNCPMNKIDYLNFRNALIEAEQATLKDFEKESANFFEACLPIEEMARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAIVQLRKEDLKGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAASGGLLAGLNASLLANNKSPVTFPDESMIGALMNFIS
NKNEIMSQQKKNKFQPMPASFGLVPELNNKIKDKKLRYKAYQKRSLEVLKEFKKVLDSSFEKDHILV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Homo sapiens, GI74024895, Length=135, Percent_Identity=34.8148148148148, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367273, Length=412, Percent_Identity=24.7572815533981, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534255, Length=437, Percent_Identity=25.629290617849, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PROM5 (A2BXA3)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011521.1
- ProteinModelPortal:   A2BXA3
- SMR:   A2BXA3
- STRING:   A2BXA3
- GeneID:   4718970
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_12071
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   PMAR167542:P9515ORF_1261-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 52361; Mature: 52361

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKEVIVIGAGLAGCEAAWQIANSGVEVKLVEMRPIHSTPAHHTSEFGELVCSNSFGAL
CCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
STDRAAGLLQEELRTFNSLIINTADEFSVPAGGALAVDRSKFSKSLTQTLSNHPLVEIKR
CCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEH
FEQLDLPDKNRITVIATGPLTSNELANKLKNFTGLDSCHFFDAASPIIYGETINYDIAFR
HHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCEECEEEEEE
ASRYDKGDPAYLNCPMNKIDYLNFRNALIEAEQATLKDFEKESANFFEACLPIEEMARRG
ECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAIVQLRKEDLKGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
SEQKRIFRMIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAASGGLLAGLNASLLANNKSPVTFPDESMIGALMNFISNKNEIMSQQKKNKFQPMPAS
HHHCCCCEEECCCHHEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
FGLVPELNNKIKDKKLRYKAYQKRSLEVLKEFKKVLDSSFEKDHILV
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLNKEVIVIGAGLAGCEAAWQIANSGVEVKLVEMRPIHSTPAHHTSEFGELVCSNSFGAL
CCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
STDRAAGLLQEELRTFNSLIINTADEFSVPAGGALAVDRSKFSKSLTQTLSNHPLVEIKR
CCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEH
FEQLDLPDKNRITVIATGPLTSNELANKLKNFTGLDSCHFFDAASPIIYGETINYDIAFR
HHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCEECEEEEEE
ASRYDKGDPAYLNCPMNKIDYLNFRNALIEAEQATLKDFEKESANFFEACLPIEEMARRG
ECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAIVQLRKEDLKGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
SEQKRIFRMIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAASGGLLAGLNASLLANNKSPVTFPDESMIGALMNFISNKNEIMSQQKKNKFQPMPAS
HHHCCCCEEECCCHHEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
FGLVPELNNKIKDKKLRYKAYQKRSLEVLKEFKKVLDSSFEKDHILV
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA