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The map label for this gene is phoB [H]

Identifier: 123966437

GI number: 123966437

Start: 1061806

End: 1062543

Strand: Direct

Name: phoB [H]

Synonym: P9515_12041

Alternate gene names: 123966437

Gene position: 1061806-1062543 (Clockwise)

Preceding gene: 123966435

Following gene: 123966442

Centisome position: 62.31

GC content: 43.9

Gene sequence:

>738_bases
ATGCAATCAACTGAGCAAATCTTAGCTTCAGCTCCTGGTAACTCCCAATTGCCCTCGACCTCTCAAACCCCTTCGAGAGT
TCTTGTTGTTGAACCTCACCCCACACTAAGAACTGTCTTAGTGCAAAGACTCCGTCAAGATGGCCATTTAGCCGCCGCAG
TCGGATCAGCGAAAGAAGCTGTAGACTTATGCAGAGAGCAGTCTCCAGACTTGTTAGTAAGCGCTGAAATATTAGAGCAA
AATACTGCCATGAGATTAGCTAAACAATTAGGTTGTTCAGTAATTGTTCTCACGGCGAGATCGGGAGTTGAAGCATTAGT
CAGCTTATTAGATGAGGGTGCTGATGACGTTTTGAGAAAACCTTTTGGATTAGAGGAATTAGCAGCAAGATGTAGAACAC
TTCTAAAAAGGGGTCGGATAGGCTTACAGGAGAAAGTTGCAGTAGGTCCTTTAGAGGTGCATCTATTACTAAGACAAGTT
ACCCTAAGTGAAAAGCCTGTAGAATTAAGCCCTAGAGAGTTTGCCTTGCTTTGTGCTCTTCTTATGCCCCCAGGGATGAT
TAGGAGCCGCCAAGAGCTTCTAAGGTTGGCTTGGCCTCCATTTAGCGGTGGACCAAGATCAGTTGACACTCAAGTTTTGA
CATTGCGTAGAAAATTAGAGCAGGCAGGATTAGGAGAGGGAGGAGGTATTACTACGGTTCGACAACAAGGATATAGATTT
AGTATTGATAATATTTAA

Upstream 100 bases:

>100_bases
CAAATGATGTCATAATTCGATAGATTCTGATTTTTAATTAGCATTTGTTATACCTTATTAAGGATAAACAAAAAATTGCC
CAGCCAAACAGTAATTAAGA

Downstream 100 bases:

>100_bases
ATTACAAAAGCTATTATTTCTCCAGCAATCATCAAAACAGACATGTAAGTTAATAATTTATATGTCCACAAAGGCGAAAT
AAACGATATTTTTTCTATAT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MQSTEQILASAPGNSQLPSTSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGSAKEAVDLCREQSPDLLVSAEILEQ
NTAMRLAKQLGCSVIVLTARSGVEALVSLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVAVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMIRSRQELLRLAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI

Sequences:

>Translated_245_residues
MQSTEQILASAPGNSQLPSTSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGSAKEAVDLCREQSPDLLVSAEILEQ
NTAMRLAKQLGCSVIVLTARSGVEALVSLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVAVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMIRSRQELLRLAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI
>Mature_245_residues
MQSTEQILASAPGNSQLPSTSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGSAKEAVDLCREQSPDLLVSAEILEQ
NTAMRLAKQLGCSVIVLTARSGVEALVSLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVAVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMIRSRQELLRLAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=31.5555555555556, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788394, Length=238, Percent_Identity=29.8319327731092, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=29.1479820627803, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.4841628959276, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=29.4642857142857, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=30.6382978723404, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790552, Length=153, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=28, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787229, Length=213, Percent_Identity=28.6384976525822, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26633; Mature: 26633

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTEQILASAPGNSQLPSTSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGSAKEA
CCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHCCHHHH
VDLCREQSPDLLVSAEILEQNTAMRLAKQLGCSVIVLTARSGVEALVSLLDEGADDVLRK
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHHC
PFGLEELAARCRTLLKRGRIGLQEKVAVGPLEVHLLLRQVTLSEKPVELSPREFALLCAL
CCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LMPPGMIRSRQELLRLAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
HCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEE
SIDNI
ECCCC
>Mature Secondary Structure
MQSTEQILASAPGNSQLPSTSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGSAKEA
CCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHCCHHHH
VDLCREQSPDLLVSAEILEQNTAMRLAKQLGCSVIVLTARSGVEALVSLLDEGADDVLRK
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHHC
PFGLEELAARCRTLLKRGRIGLQEKVAVGPLEVHLLLRQVTLSEKPVELSPREFALLCAL
CCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LMPPGMIRSRQELLRLAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
HCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEE
SIDNI
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]