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The map label for this gene is folD

Identifier: 123966392

GI number: 123966392

Start: 1012865

End: 1013761

Strand: Direct

Name: folD

Synonym: P9515_11591

Alternate gene names: 123966392

Gene position: 1012865-1013761 (Clockwise)

Preceding gene: 123966390

Following gene: 123966393

Centisome position: 59.43

GC content: 30.88

Gene sequence:

>897_bases
ATGTCATTAAAATTGGATGGTAAAAAATTATCTTTAGAAATTGAAGAAAAATTAAAAAAAGATATTGAATCTAATATAAA
TTCAACAAAAAGGCCTCCAGGTTTAGCTGTAATAAGAATTGGAGAAGATCCTGCTAGTGGCGTCTATGTTAAGAATAAAG
AAAGAGCATGTGCAAGAGTTGGGATAAAGAGTTTTATTTTTCATTTGAAAGATACTATCGATCAAAAAGAAGTTGAACAA
TTAATAAATAAATTAAATCTTGATAATGATATTGACGGAATGCTACTGCAACTTCCCATACCAGATAAATTTGATGAGCA
AAGATTGATAAGTTTAATCAATCCAGACAAAGATGTAGATGGATTAAATGAGAAAAATATTGGCAAATTAGTTAAAAATG
AACCGGGGATGAGATCTTGTACACCAGCAGGGATAGTTAATTTATTAAGATCCCAAAATATTACAATTGAAGGAAAAAAA
ATTGTTGTTGTTGGAAGAAGCCTCTTGGTTGGGAAACCCCTATCTCTAATGTTGTTAAATCTAAATGGTACGGTAACAAT
AACTCATTCAAAGACTATAAATTTAAAGAAGATATGTAAGGAAGCGGATATATTAATTGCTGCCGCAGGAAAACCTAATC
TTATAAACTCTAGTTATGTAAAAGATGGTGCTGTAATTATTGATGTGGGAATACATAGATTAACAAGTTCTGATAAAAGC
CAAACTAAATTATGTGGAGATGTATTATTAGAAGATGTAATTCCCAAAGTTTATGCATACACACCGGTTCCTGGAGGTGT
TGGACCAATGACGGTAACAATGTTACTAGTAAATACTATTTTTAGTTGGCAAAAGCAATTTGTTTTATTATCAAGGCTTA
GTGACCTTTTGCCATAA

Upstream 100 bases:

>100_bases
TTTAAAGTAGTTTTAAGGTTATGCACAGAATATGAAGAAATCTATTTGAATAATTTAAAATTATAGGCATGCTAGGTGTA
AAAAGGAGAAGTTTTTCTAA

Downstream 100 bases:

>100_bases
AGTCCACGATGCAGAAAAAATTTACTCAATGTAAAAAATGACAGAAGTTATCGATAATATTTCAGATTTTGATAAATATC
TAAAAGAGACAAAAAAAATC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLKLDGKKLSLEIEEKLKKDIESNINSTKRPPGLAVIRIGEDPASGVYVKNKERACARVGIKSFIFHLKDTIDQKEVEQ
LINKLNLDNDIDGMLLQLPIPDKFDEQRLISLINPDKDVDGLNEKNIGKLVKNEPGMRSCTPAGIVNLLRSQNITIEGKK
IVVVGRSLLVGKPLSLMLLNLNGTVTITHSKTINLKKICKEADILIAAAGKPNLINSSYVKDGAVIIDVGIHRLTSSDKS
QTKLCGDVLLEDVIPKVYAYTPVPGGVGPMTVTMLLVNTIFSWQKQFVLLSRLSDLLP

Sequences:

>Translated_298_residues
MSLKLDGKKLSLEIEEKLKKDIESNINSTKRPPGLAVIRIGEDPASGVYVKNKERACARVGIKSFIFHLKDTIDQKEVEQ
LINKLNLDNDIDGMLLQLPIPDKFDEQRLISLINPDKDVDGLNEKNIGKLVKNEPGMRSCTPAGIVNLLRSQNITIEGKK
IVVVGRSLLVGKPLSLMLLNLNGTVTITHSKTINLKKICKEADILIAAAGKPNLINSSYVKDGAVIIDVGIHRLTSSDKS
QTKLCGDVLLEDVIPKVYAYTPVPGGVGPMTVTMLLVNTIFSWQKQFVLLSRLSDLLP
>Mature_297_residues
SLKLDGKKLSLEIEEKLKKDIESNINSTKRPPGLAVIRIGEDPASGVYVKNKERACARVGIKSFIFHLKDTIDQKEVEQL
INKLNLDNDIDGMLLQLPIPDKFDEQRLISLINPDKDVDGLNEKNIGKLVKNEPGMRSCTPAGIVNLLRSQNITIEGKKI
VVVGRSLLVGKPLSLMLLNLNGTVTITHSKTINLKKICKEADILIAAAGKPNLINSSYVKDGAVIIDVGIHRLTSSDKSQ
TKLCGDVLLEDVIPKVYAYTPVPGGVGPMTVTMLLVNTIFSWQKQFVLLSRLSDLLP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=299, Percent_Identity=43.4782608695652, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=40.4109589041096, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=36.5853658536585, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI36796743, Length=201, Percent_Identity=27.8606965174129, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=43.8405797101449, Blast_Score=243, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=282, Percent_Identity=39.7163120567376, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=40.1360544217687, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=39.5973154362416, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=320, Percent_Identity=25, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=40.7407407407407, Blast_Score=210, Evalue=9e-55,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=40.7407407407407, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI62472483, Length=304, Percent_Identity=35.1973684210526, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI45551871, Length=304, Percent_Identity=35.1973684210526, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24645718, Length=295, Percent_Identity=36.6101694915254, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI17137370, Length=295, Percent_Identity=36.6101694915254, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PROM5 (A2BX55)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011473.1
- ProteinModelPortal:   A2BX55
- SMR:   A2BX55
- STRING:   A2BX55
- GeneID:   4718979
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_11591
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14186
- BioCyc:   PMAR167542:P9515ORF_1211-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32709; Mature: 32578

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKLDGKKLSLEIEEKLKKDIESNINSTKRPPGLAVIRIGEDPASGVYVKNKERACARV
CCEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHH
GIKSFIFHLKDTIDQKEVEQLINKLNLDNDIDGMLLQLPIPDKFDEQRLISLINPDKDVD
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEKNIGKLVKNEPGMRSCTPAGIVNLLRSQNITIEGKKIVVVGRSLLVGKPLSLMLLN
CCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTITHSKTINLKKICKEADILIAAAGKPNLINSSYVKDGAVIIDVGIHRLTSSDKS
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEECHHHHCCCCCH
QTKLCGDVLLEDVIPKVYAYTPVPGGVGPMTVTMLLVNTIFSWQKQFVLLSRLSDLLP
HHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLKLDGKKLSLEIEEKLKKDIESNINSTKRPPGLAVIRIGEDPASGVYVKNKERACARV
CEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHH
GIKSFIFHLKDTIDQKEVEQLINKLNLDNDIDGMLLQLPIPDKFDEQRLISLINPDKDVD
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEKNIGKLVKNEPGMRSCTPAGIVNLLRSQNITIEGKKIVVVGRSLLVGKPLSLMLLN
CCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTITHSKTINLKKICKEADILIAAAGKPNLINSSYVKDGAVIIDVGIHRLTSSDKS
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEECHHHHCCCCCH
QTKLCGDVLLEDVIPKVYAYTPVPGGVGPMTVTMLLVNTIFSWQKQFVLLSRLSDLLP
HHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA