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The map label for this gene is leuA [H]

Identifier: 123966389

GI number: 123966389

Start: 1009192

End: 1010832

Strand: Direct

Name: leuA [H]

Synonym: P9515_11561

Alternate gene names: 123966389

Gene position: 1009192-1010832 (Clockwise)

Preceding gene: 123966387

Following gene: 123966390

Centisome position: 59.22

GC content: 35.04

Gene sequence:

>1641_bases
ATGTCCAAAGATCCAGGCAGAATATTGATCTTTGATACAACTCTTAGAGATGGAGAACAATCTCCTGGCGCAAGTTTAAA
TCTTGAAGAGAAACTTGCAATTGCGCATCAACTTGCAAGATTAGGTGTAGATGTTATTGAAGCAGGTTTTCCTTTTGCAA
GTCCTGGAGATTTCAAAGCAGTAAATAAAATTTCAGAAGTAGTTGGAGGAGAAAATGGTCCAATAATTTGTGGTTTAGCA
AGGGCTTCTACAAGTGATATAAAAGCTTGTTACGAAGCCATAATTCCCGCTCCTAAAAAAAGAATACATACTTTCATAGC
TACTAGCGATATCCACCTAAAACATAAACTCAGGAAATCAAGAAAAGATGTTCTTTCAATTGTTCCTGAAATGGTCAGTT
ATGCTAAATCATTAGTCGATGATATTGAATTTTCCTGTGAAGATGCATCAAGGAGTGATCCCGAATTCCTTTATGAAGTA
ATTCAACTAGCAATAACATCAGGTGCTACAACAATTAATATTCCTGACACTGTTGGCTTCACGACTCCGAGTGAATTTGG
GAAGCTTATTTTTGATATTAATAAAAATGTTCCAAACATTGATGAGGCAGTTATTTCCGTTCATGGTCATAATGATTTAG
GATTGGCAGTTGCAAATTTCCTAGAAGCAGCAAAAAATGGAGCAAGGCAACTTGAATGCACCATTAATGGTATAGGTGAG
AGAGCTGGAAATGCATCACTAGAAGAATTAGTAATGGCATTACACGTAAGAAAAAGCTTTTTTAATAGTTTCTTTGGAAG
AAGTTCAGATTCACCCACGCCTCTTACAGCAATAAGAACAGAAGAAATAACAAAAACTTCACGGTTAGTATCAAACTTAA
CTGGAATGAATGTTCAGCCCAATAAAGCTATTGTTGGAGCAAATGCTTTTGCTCATGAGTCAGGAATCCATCAGGATGGA
GTACTAAAAAATAGGCTTACGTATGAAATTATTGATGCAAAAACTGTTGGTTTGAATGATAATAAAATCTCTTTAGGGAA
ACTTAGTGGTAGAAGTGCAGTTAGAGCAAGATTAGAAGAGATGGGATACGATTTAAGTAGGGAAGATTTGAATGATGCAT
TTGCTCGTTTCAAAGATCTAGCTGATAGAAAACGAGAAATTACTGATAGAGATTTAGAAGCTATCGTTAGTGAACAGGTT
CAACTTCCTGAATCTAGGTTTCAACTAAGTCACGTTCAAGTTAGTTGTGGCAGTACATCTAAGCCTACAGCTACAGTTAC
TATATTAAATACAGAGACACATACTGAAGATACTTCTGTTGCCATAGGTACTGGACCTGTAGATGCGGTTTGCGAAGCAA
TAAATGAACTTGCTAAAGTTCCAAATGAATTAATTGAGTTTTCAGTTAAATCTGTGACGGAAGGTATAGATGCATTAGGT
GAAGTTACCATAAGAATTAGGAATAAGAATAAAATATATTCAGGACATTCTGCAGATACAGACGTGGTTGTTGCAGCTGC
AAATGCTTTTTTAAATGCTTTAAACAGGCTTATCTTTTCAGAAAAAAAAGAATGTATTCATCCCCAATTTGATAATTTAG
AAAATTCTGAAAAAAAAGTATTTTCAAATCCCAAAAATTAA

Upstream 100 bases:

>100_bases
TAATGTATGAATATGATTTCTTTACTTTTAATTTAAAATTAATCCAAATCTAGTAATTTTTTTTTAATTGGCTTAATATG
GATAAAGATACATTTAGTTA

Downstream 100 bases:

>100_bases
ATTAATTTTGGGATTATTAACTTTAGCTAAAATCTCGTTACCAATAATGTAGATTAAAGGTCGAAGTTTAAATTTAGTCA
AATAATGAGAGAAAGGGTAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISEVVGGENGPIICGLA
RASTSDIKACYEAIIPAPKKRIHTFIATSDIHLKHKLRKSRKDVLSIVPEMVSYAKSLVDDIEFSCEDASRSDPEFLYEV
IQLAITSGATTINIPDTVGFTTPSEFGKLIFDINKNVPNIDEAVISVHGHNDLGLAVANFLEAAKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFGRSSDSPTPLTAIRTEEITKTSRLVSNLTGMNVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLNDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPESRFQLSHVQVSCGSTSKPTATVTILNTETHTEDTSVAIGTGPVDAVCEAINELAKVPNELIEFSVKSVTEGIDALG
EVTIRIRNKNKIYSGHSADTDVVVAAANAFLNALNRLIFSEKKECIHPQFDNLENSEKKVFSNPKN

Sequences:

>Translated_546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISEVVGGENGPIICGLA
RASTSDIKACYEAIIPAPKKRIHTFIATSDIHLKHKLRKSRKDVLSIVPEMVSYAKSLVDDIEFSCEDASRSDPEFLYEV
IQLAITSGATTINIPDTVGFTTPSEFGKLIFDINKNVPNIDEAVISVHGHNDLGLAVANFLEAAKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFGRSSDSPTPLTAIRTEEITKTSRLVSNLTGMNVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLNDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPESRFQLSHVQVSCGSTSKPTATVTILNTETHTEDTSVAIGTGPVDAVCEAINELAKVPNELIEFSVKSVTEGIDALG
EVTIRIRNKNKIYSGHSADTDVVVAAANAFLNALNRLIFSEKKECIHPQFDNLENSEKKVFSNPKN
>Mature_545_residues
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKISEVVGGENGPIICGLAR
ASTSDIKACYEAIIPAPKKRIHTFIATSDIHLKHKLRKSRKDVLSIVPEMVSYAKSLVDDIEFSCEDASRSDPEFLYEVI
QLAITSGATTINIPDTVGFTTPSEFGKLIFDINKNVPNIDEAVISVHGHNDLGLAVANFLEAAKNGARQLECTINGIGER
AGNASLEELVMALHVRKSFFNSFFGRSSDSPTPLTAIRTEEITKTSRLVSNLTGMNVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDAKTVGLNDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQ
LPESRFQLSHVQVSCGSTSKPTATVTILNTETHTEDTSVAIGTGPVDAVCEAINELAKVPNELIEFSVKSVTEGIDALGE
VTIRIRNKNKIYSGHSADTDVVVAAANAFLNALNRLIFSEKKECIHPQFDNLENSEKKVFSNPKN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=46.7836257309941, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=30.2325581395349, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=389, Percent_Identity=29.5629820051414, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=558, Percent_Identity=25.9856630824373, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324682, Length=504, Percent_Identity=24.6031746031746, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59387; Mature: 59256

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKISEVVGGENGPIICGLARASTSDIKACYEAIIPAPKKRIHTFIATSDIHLKHKLRKS
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
RKDVLSIVPEMVSYAKSLVDDIEFSCEDASRSDPEFLYEVIQLAITSGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGKLIFDINKNVPNIDEAVISVHGHNDLGLAVANFLEAAKNGARQLECTINGIGE
CCHHHHCCEEEEECCCCCCCCHHEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFGRSSDSPTPLTAIRTEEITKTSRLVSNLTGMNVQP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLNDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCEEEEECCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPESRFQLSHVQVSCGSTS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCC
KPTATVTILNTETHTEDTSVAIGTGPVDAVCEAINELAKVPNELIEFSVKSVTEGIDALG
CCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRNKNKIYSGHSADTDVVVAAANAFLNALNRLIFSEKKECIHPQFDNLENSEKKV
EEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FSNPKN
CCCCCC
>Mature Secondary Structure 
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKISEVVGGENGPIICGLARASTSDIKACYEAIIPAPKKRIHTFIATSDIHLKHKLRKS
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
RKDVLSIVPEMVSYAKSLVDDIEFSCEDASRSDPEFLYEVIQLAITSGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGKLIFDINKNVPNIDEAVISVHGHNDLGLAVANFLEAAKNGARQLECTINGIGE
CCHHHHCCEEEEECCCCCCCCHHEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFGRSSDSPTPLTAIRTEEITKTSRLVSNLTGMNVQP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLNDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCEEEEECCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPESRFQLSHVQVSCGSTS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCC
KPTATVTILNTETHTEDTSVAIGTGPVDAVCEAINELAKVPNELIEFSVKSVTEGIDALG
CCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRNKNKIYSGHSADTDVVVAAANAFLNALNRLIFSEKKECIHPQFDNLENSEKKV
EEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FSNPKN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA