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The map label for this gene is hisH [H]

Identifier: 123966383

GI number: 123966383

Start: 1001051

End: 1001668

Strand: Reverse

Name: hisH [H]

Synonym: P9515_11501

Alternate gene names: 123966383

Gene position: 1001668-1001051 (Counterclockwise)

Preceding gene: 123966384

Following gene: 123966382

Centisome position: 58.78

GC content: 29.61

Gene sequence:

>618_bases
TTGTCTAAAATAGGACTTATAGATTACGGAATGGGTAACATTCATTCCGTGACGAAAGCCTTAGAAAATCTTGAAGAAGA
AATACTTTTAATTAAAAATAAGCACCAAACAAAAGATTGTAAGGCTTTAATACTACCTGGGGTTGGTTCATTCGATCCGG
CAATAAATAATCTAATTAATACTGATTTAATAATTGATATTAAAAGCTGGATTAATAGTGGAAAATCATTTTTAGGAATT
TGTCTTGGATTACAATTGCTTTTTGAATCAAGTGATGAAGGCTCAATAAATGGACTTGGCATAGTTAAAGGCCAAATAAA
AAAAATTCCTCAATTACCTAATCAAAGAATTCCTCATGTTGGATGGTGTGAACTTCTTCCAACAAGAAAAAATAGCTTAT
TAAGAAATGAAGAATTAAATAATTGGGTATATTTTGTTCATTCTTATCATGCTGTTCCTTCAAATACTGATGTAATAGCT
GCAAATGTAAGTTATGGTTCTGAAAAATTAACTGCAATAATTGAACAAGATAATTTACTTGCTTGTCAGTTTCATCCTGA
AAAGTCAGGAAAAACAGGAGAAAAACTTTTACGTAGATGGCTTAAAAATATTCAATAA

Upstream 100 bases:

>100_bases
AATGATATTTAAAGGTGGTCAAAAGGTTGATACTGTCGTTGGAGCTGTTCCAAAAGCGACTCTTTCAAGTACCTTGTCTA
AGCACTTATAAATTAAAGAA

Downstream 100 bases:

>100_bases
TTCTATGTGATATATGAAATCAAATTTAAGGTTAATTGGAGGAAGAAAGCTTGAGAGCCCAAATACTAATGAATCTAGGC
CAACTACTTTAATGGTTAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSKIGLIDYGMGNIHSVTKALENLEEEILLIKNKHQTKDCKALILPGVGSFDPAINNLINTDLIIDIKSWINSGKSFLGI
CLGLQLLFESSDEGSINGLGIVKGQIKKIPQLPNQRIPHVGWCELLPTRKNSLLRNEELNNWVYFVHSYHAVPSNTDVIA
ANVSYGSEKLTAIIEQDNLLACQFHPEKSGKTGEKLLRRWLKNIQ

Sequences:

>Translated_205_residues
MSKIGLIDYGMGNIHSVTKALENLEEEILLIKNKHQTKDCKALILPGVGSFDPAINNLINTDLIIDIKSWINSGKSFLGI
CLGLQLLFESSDEGSINGLGIVKGQIKKIPQLPNQRIPHVGWCELLPTRKNSLLRNEELNNWVYFVHSYHAVPSNTDVIA
ANVSYGSEKLTAIIEQDNLLACQFHPEKSGKTGEKLLRRWLKNIQ
>Mature_204_residues
SKIGLIDYGMGNIHSVTKALENLEEEILLIKNKHQTKDCKALILPGVGSFDPAINNLINTDLIIDIKSWINSGKSFLGIC
LGLQLLFESSDEGSINGLGIVKGQIKKIPQLPNQRIPHVGWCELLPTRKNSLLRNEELNNWVYFVHSYHAVPSNTDVIAA
NVSYGSEKLTAIIEQDNLLACQFHPEKSGKTGEKLLRRWLKNIQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=35.3535353535354, Blast_Score=110, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=33.8028169014084, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22883; Mature: 22752

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIGLIDYGMGNIHSVTKALENLEEEILLIKNKHQTKDCKALILPGVGSFDPAINNLIN
CCCCCEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCCCCHHHHHHCC
TDLIIDIKSWINSGKSFLGICLGLQLLFESSDEGSINGLGIVKGQIKKIPQLPNQRIPHV
CCEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCC
GWCELLPTRKNSLLRNEELNNWVYFVHSYHAVPSNTDVIAANVSYGSEKLTAIIEQDNLL
CHHHCCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLKNIQ
EEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIGLIDYGMGNIHSVTKALENLEEEILLIKNKHQTKDCKALILPGVGSFDPAINNLIN
CCCCEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCCCCHHHHHHCC
TDLIIDIKSWINSGKSFLGICLGLQLLFESSDEGSINGLGIVKGQIKKIPQLPNQRIPHV
CCEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCC
GWCELLPTRKNSLLRNEELNNWVYFVHSYHAVPSNTDVIAANVSYGSEKLTAIIEQDNLL
CHHHCCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLKNIQ
EEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]