Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 123966246

GI number: 123966246

Start: 906847

End: 907830

Strand: Reverse

Name: pdhB [H]

Synonym: P9515_10131

Alternate gene names: 123966246

Gene position: 907830-906847 (Counterclockwise)

Preceding gene: 123966247

Following gene: 123966245

Centisome position: 53.27

GC content: 34.04

Gene sequence:

>984_bases
GTGGCTAGTACTTTATTATTTAATGCTTTGAAAGAAGCAATTGATGAAGAGATGGCAAATGATGTAAACGTTTGCGTTAT
GGGAGAAGATGTAGGCCAATATGGTGGTTCTTATAAAGTCACTAAAGATCTATATGAAAAATATGGCGAATTAAGAGTTT
TAGATACTCCAATTGCCGAAAATAGTTTTACGGGGATGGCAGTTGGTGCTGCAATGACAGGATTAAGACCGATAGTGGAA
GGTATGAACATGGGATTTCTACTATTAGCCTTTAATCAAATTTCTAATAATATGGGCATGCTCAGATATACAAGTGGGGG
CAATTATAAAATACCTGCAGTTGTTCGTGGCCCTGGCGGTGTTGGGAGACAACTAGGCGCTGAACATAGTCAAAGATTAG
AGGCTTACTTTCACGCAGTTCCTGGGATCAAGATAGTTGCTTGTAGTACCCCCACAAATGCAAAAGGATTAATGAAAGCT
GCTATAAGGGACAATAATCCCGTTTTATTTTTTGAACATGTCCTTTTATACAATTTATCTGAGGAATTGCCTGAGGGAGA
TTATATCTGTTCTTTAGATCAAGCTGACATAGTCAAAGAGGGGAAAGATATTACGATTCTTACCTATTCAAGAATGAGAC
ATCATTGTTTAAAAGCTATTGAAGAGTTAGATAAGAAGAATATCGATGTCGAATTAATAGATCTGATAAGTTTAAAACCT
TTTGATATGAAAACAATTTCGAAATCTATTAAAAAAACTAATAATGTAATTATTGTAGAAGAATGTATGAAAACTGGAGG
AATTGGAGCAGAATTGATTGCTTTGATTACAGAAGAATGTTTTGATGATCTTGATCATAGACCTATAAGATTATCTAGTC
AGGATATTCCTACTCCTTATAATGGTAATTTAGAAAATTTAACAATAATTCAACCGCATCAAATAGTCGAAAAAGTAGAA
GAAATAATCAATGGGAGTATTTAA

Upstream 100 bases:

>100_bases
CATGATTTTGTCAATATTACTACTTATAACTTTTGTAGAAGGAGGCTAATTTAACCTTGATGGTATAAATTTATATATAT
GATCAATTAGGGAATTACTC

Downstream 100 bases:

>100_bases
GAAATGAAAAAAAGGCAAGGTTGGTTTTTTTTTATTATTTTCTTACTGACTTTATCTATTTATTTATTAATTAATTTCCC
ATTACAATTAGGTCTAGATT

Product: pyruvate dehydrogenase E1 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MASTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLSEELPEGDYICSLDQADIVKEGKDITILTYSRMRHHCLKAIEELDKKNIDVELIDLISLKP
FDMKTISKSIKKTNNVIIVEECMKTGGIGAELIALITEECFDDLDHRPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
EIINGSI

Sequences:

>Translated_327_residues
MASTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLSEELPEGDYICSLDQADIVKEGKDITILTYSRMRHHCLKAIEELDKKNIDVELIDLISLKP
FDMKTISKSIKKTNNVIIVEECMKTGGIGAELIALITEECFDDLDHRPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
EIINGSI
>Mature_326_residues
ASTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVEG
MNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAA
IRDNNPVLFFEHVLLYNLSEELPEGDYICSLDQADIVKEGKDITILTYSRMRHHCLKAIEELDKKNIDVELIDLISLKPF
DMKTISKSIKKTNNVIIVEECMKTGGIGAELIALITEECFDDLDHRPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVEE
IINGSI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=322, Percent_Identity=40.0621118012422, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI291084858, Length=322, Percent_Identity=37.888198757764, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI4557353, Length=317, Percent_Identity=35.0157728706625, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI34101272, Length=317, Percent_Identity=35.0157728706625, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17538422, Length=329, Percent_Identity=40.4255319148936, Blast_Score=262, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17506935, Length=322, Percent_Identity=33.5403726708075, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319698, Length=322, Percent_Identity=42.5465838509317, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=38.4615384615385, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=38.4615384615385, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=37.1517027863777, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=37.1517027863777, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650943, Length=85, Percent_Identity=51.7647058823529, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24650945, Length=85, Percent_Identity=51.7647058823529, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36086; Mature: 35955

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHH
EELPEGDYICSLDQADIVKEGKDITILTYSRMRHHCLKAIEELDKKNIDVELIDLISLKP
HHCCCCCEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDMKTISKSIKKTNNVIIVEECMKTGGIGAELIALITEECFDDLDHRPIRLSSQDIPTPY
CCHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEEIINGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHH
EELPEGDYICSLDQADIVKEGKDITILTYSRMRHHCLKAIEELDKKNIDVELIDLISLKP
HHCCCCCEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDMKTISKSIKKTNNVIIVEECMKTGGIGAELIALITEECFDDLDHRPIRLSSQDIPTPY
CCHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEEIINGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]