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The map label for this gene is pykF [H]

Identifier: 123966228

GI number: 123966228

Start: 888621

End: 890411

Strand: Direct

Name: pykF [H]

Synonym: P9515_09951

Alternate gene names: 123966228

Gene position: 888621-890411 (Clockwise)

Preceding gene: 123966226

Following gene: 123966229

Centisome position: 52.14

GC content: 34.45

Gene sequence:

>1791_bases
ATGTCGAATATTGATTTAAAAAGAAGAACAAAAATTGTAGCAACAATTGGACCAGCTACTCAGTCTGAAGAAGTAATTAC
TGATCTTATAAAGGCTGGGGTAACAACATTCCGATTAAATTTTTCCCATGGCGATCATAAAGATCATGAAGAAAGGATTA
AAACTATAAGAAAAGTTTCTAAAAATCTTGATCTAGATGTAGGAATACTACAAGATCTGCAAGGACCTAAAATAAGACTA
GGAAGATTTAAAGATGGTCCCGTTAAAGTAAAAAAAGGAGATAAATTTACATTGACTTCTAACGAAGTTGAATGCACTAA
AAGTATTGCAAACGTTACTTATAATAGACTCGCGCAGGAAGTTACCGAAGGCAAAAGAATATTATTAGACGATGGTAAAA
TTGAAATGATTGTTGAAAAGGTTGACTTACCAAATAATTTATTAAAGTGTAAAGTAACTGTCGGTGGGGTACTTTCTAAC
AATAAAGGAGTTAATTTTCCAGATGTGCAATTATCGGTAAAAGCTTTAACTGATAAAGATATAGATGATCTTGAATTTGG
ATTAACCGCTGGAGTAGATTGGATAGCTCTAAGTTTTGTAAGAAACCCCTCAGATATTAATGAAATAAAAAATTTAATAA
ATAAGAGTGGGCAATCAATACCTGTGATAGCAAAAATTGAAAAATTTGAAGCAATAGATCAAATAGATTCAATACTACCC
TTGTGTGATGGAGTTATGGTTGCAAGAGGAGATTTAGGAGTTGAAATGCCAGCGGAGGAAGTTCCACTTTTACAAAAAGA
GTTAATAAGGAAAGCTAATACATTTGGGATCCCTATAATCACGGCTACACAAATGCTTGACTCCATGGCATCAAATCCCA
GACCAACACGAGCGGAAGTTAGTGATGTGGCTAACGCAATTCTTGATGGGACTGATGCAGTTATGCTCTCAAATGAAACT
GCCGTAGGTGACTATCCTGTAGAGGCTGTTGAAACTATGGCTACTATTGCCAGAAGAATTGAAAGAGATTATCCATTAAA
AGCAATCGAGAGTAATCTGCCTAGTACTATCCCAAATGCAATAAGTGCTGCAGTAAGCAATATTGCGAGGCAGCTTGACG
CTGGAGCAATAATTCCTTTAACTAAATCAGGATCCACAGCACGTAATGTAAGTAAGTTCAGACCACCTACTCCTATCCTT
GCCACGACTACTGAGAAGAGTGTCGCAAGAAGATTACAACTTGTGTGGGGAGTGACACCATTATTAGTAAAATCTGACGA
TAGAACAGCAAGAACATTTAGTATTGCAATGCAAATAGCTCAAGAAATGGGGATTCTTAAGCAAGGAGACTTAGTAGTAC
AAACTGCTGGAACTTTGACAGGAATAAGTGGATCTACTGACCTTATAAAAGTTGGCCTAGTAAGAAAAGTGATAACCCGA
GGAATATCAATTGGAGAAATAGGTGTTACAGGTAAAGCGAGAAATATAGAAACAAGTCTTGATTTATCATTAATAACTCA
CGGTGAAATATTATTTGTATCTAAGAATCTATTAAAAGATTTACCTTACTCAAAAAAAATCACAGGCATCGTTACTGATG
AAACTGTTGATGAATGTTATAAGATTTTTAAAAAAAATAAAATTAAAATATCTTCAATTTGTAATTTATCTACTATTGAT
AATCAGGTAACAAATGGTGATGTAATAACACTACAACTTAATGAAGGAGTAATTTATATGGGACAGATAGAGGAAGATGA
AGAGGCACTAGACAGATATAAATATGTCTAG

Upstream 100 bases:

>100_bases
TGTCAATCGAGCTTTTTTCTGATAAGTTTTGAAATCCATAGATTAAATAACTAACTTTAGGTATGTTAAATTTATTTATA
TCTAGCAATATTATTTATAT

Downstream 100 bases:

>100_bases
GAATATATCTTTTAAAGAAGCTTTTAAAATGGCTTGCAAAACATTAGTTTCTAATAAATTGAGAAGTTCCCTTACTATGT
TGGGGATAGTTATAGGAAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSNIDLKRRTKIVATIGPATQSEEVITDLIKAGVTTFRLNFSHGDHKDHEERIKTIRKVSKNLDLDVGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECTKSIANVTYNRLAQEVTEGKRILLDDGKIEMIVEKVDLPNNLLKCKVTVGGVLSN
NKGVNFPDVQLSVKALTDKDIDDLEFGLTAGVDWIALSFVRNPSDINEIKNLINKSGQSIPVIAKIEKFEAIDQIDSILP
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANTFGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVETMATIARRIERDYPLKAIESNLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTEKSVARRLQLVWGVTPLLVKSDDRTARTFSIAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKVITR
GISIGEIGVTGKARNIETSLDLSLITHGEILFVSKNLLKDLPYSKKITGIVTDETVDECYKIFKKNKIKISSICNLSTID
NQVTNGDVITLQLNEGVIYMGQIEEDEEALDRYKYV

Sequences:

>Translated_596_residues
MSNIDLKRRTKIVATIGPATQSEEVITDLIKAGVTTFRLNFSHGDHKDHEERIKTIRKVSKNLDLDVGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECTKSIANVTYNRLAQEVTEGKRILLDDGKIEMIVEKVDLPNNLLKCKVTVGGVLSN
NKGVNFPDVQLSVKALTDKDIDDLEFGLTAGVDWIALSFVRNPSDINEIKNLINKSGQSIPVIAKIEKFEAIDQIDSILP
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANTFGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVETMATIARRIERDYPLKAIESNLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTEKSVARRLQLVWGVTPLLVKSDDRTARTFSIAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKVITR
GISIGEIGVTGKARNIETSLDLSLITHGEILFVSKNLLKDLPYSKKITGIVTDETVDECYKIFKKNKIKISSICNLSTID
NQVTNGDVITLQLNEGVIYMGQIEEDEEALDRYKYV
>Mature_595_residues
SNIDLKRRTKIVATIGPATQSEEVITDLIKAGVTTFRLNFSHGDHKDHEERIKTIRKVSKNLDLDVGILQDLQGPKIRLG
RFKDGPVKVKKGDKFTLTSNEVECTKSIANVTYNRLAQEVTEGKRILLDDGKIEMIVEKVDLPNNLLKCKVTVGGVLSNN
KGVNFPDVQLSVKALTDKDIDDLEFGLTAGVDWIALSFVRNPSDINEIKNLINKSGQSIPVIAKIEKFEAIDQIDSILPL
CDGVMVARGDLGVEMPAEEVPLLQKELIRKANTFGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNETA
VGDYPVEAVETMATIARRIERDYPLKAIESNLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILA
TTTEKSVARRLQLVWGVTPLLVKSDDRTARTFSIAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKVITRG
ISIGEIGVTGKARNIETSLDLSLITHGEILFVSKNLLKDLPYSKKITGIVTDETVDECYKIFKKNKIKISSICNLSTIDN
QVTNGDVITLQLNEGVIYMGQIEEDEEALDRYKYV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=40.3688524590164, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=40.2040816326531, Blast_Score=332, Evalue=8e-91,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=40.2040816326531, Blast_Score=332, Evalue=9e-91,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=39.7540983606557, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=39.7540983606557, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=40.1384083044983, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=40.1384083044983, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.7088607594937, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.7088607594937, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=35.6481481481481, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=44.136460554371, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1788160, Length=473, Percent_Identity=41.2262156448203, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=39.0852390852391, Blast_Score=328, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=38.8090349075975, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.8090349075975, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.8090349075975, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=38.8090349075975, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=39.6303901437372, Blast_Score=303, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6324923, Length=479, Percent_Identity=38.8308977035491, Blast_Score=302, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=39.7540983606557, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=39.7540983606557, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=40.3940886699507, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=33.8809034907598, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24646914, Length=329, Percent_Identity=38.9057750759878, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 65071; Mature: 64940

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIDLKRRTKIVATIGPATQSEEVITDLIKAGVTTFRLNFSHGDHKDHEERIKTIRKVS
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
KNLDLDVGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECTKSIANVTYNRLAQE
HCCCCCHHHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
VTEGKRILLDDGKIEMIVEKVDLPNNLLKCKVTVGGVLSNNKGVNFPDVQLSVKALTDKD
HHCCCEEEEECCCEEEEEEECCCCCCEEEEEEEECEEEECCCCCCCCCCEEEEEECCCCC
IDDLEFGLTAGVDWIALSFVRNPSDINEIKNLINKSGQSIPVIAKIEKFEAIDQIDSILP
CHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANTFGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVETMATIARRIERDYPLKAIESNLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
ISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTEKSVARRLQLVWGVTP
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
LLVKSDDRTARTFSIAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKVITR
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GISIGEIGVTGKARNIETSLDLSLITHGEILFVSKNLLKDLPYSKKITGIVTDETVDECY
CCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHHHHHCCCCCCEEEEECHHHHHHHH
KIFKKNKIKISSICNLSTIDNQVTNGDVITLQLNEGVIYMGQIEEDEEALDRYKYV
HHHHCCCEEEECCCCCHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
SNIDLKRRTKIVATIGPATQSEEVITDLIKAGVTTFRLNFSHGDHKDHEERIKTIRKVS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
KNLDLDVGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECTKSIANVTYNRLAQE
HCCCCCHHHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
VTEGKRILLDDGKIEMIVEKVDLPNNLLKCKVTVGGVLSNNKGVNFPDVQLSVKALTDKD
HHCCCEEEEECCCEEEEEEECCCCCCEEEEEEEECEEEECCCCCCCCCCEEEEEECCCCC
IDDLEFGLTAGVDWIALSFVRNPSDINEIKNLINKSGQSIPVIAKIEKFEAIDQIDSILP
CHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANTFGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVETMATIARRIERDYPLKAIESNLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
ISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTEKSVARRLQLVWGVTP
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
LLVKSDDRTARTFSIAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKVITR
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GISIGEIGVTGKARNIETSLDLSLITHGEILFVSKNLLKDLPYSKKITGIVTDETVDECY
CCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHHHHHCCCCCCEEEEECHHHHHHHH
KIFKKNKIKISSICNLSTIDNQVTNGDVITLQLNEGVIYMGQIEEDEEALDRYKYV
HHHHCCCEEEECCCCCHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]