Click here to switch to the map view.

The map label for this gene is ribB [H]

Identifier: 123966208

GI number: 123966208

Start: 871661

End: 873394

Strand: Direct

Name: ribB [H]

Synonym: P9515_09751

Alternate gene names: 123966208

Gene position: 871661-873394 (Clockwise)

Preceding gene: 123966206

Following gene: 123966213

Centisome position: 51.15

GC content: 32.3

Gene sequence:

>1734_bases
ATGAAAGAAACAAGTCCCAAATCAAATAATGGAACAATTTTGGATAATAATGAAAAGTTTAAAATAGAATTTGATCAGAT
AAGCGATGCATTAGCTGCTATAAGGAATGGAGAATGCATCATTGTAGTTGATGATGAAAGAAGAGAAAATGAAGGAGATT
TAATTTGCGCTGCTCAGTTTGCAACTCCTCAACAAATTAACTTTATGGCAACTGAAGGAAGAGGACTTATATGCTTAGCA
ATGCAAGGAGATAAATTAGATGCATTAGATCTTCCTTTAATGGTTGATCGAAATACTGATAAGAATCAAACTGCCTTTAC
TGTATCAATAGATGCTGGGCCAGAGAATAATGTAACTACAGGCATATCAGCAGAGGATAGAGCTAAAACTATACAAGTAG
CAATAAATCCTTCAACGAAACCTGAAGATTTAAGAAGACCTGGACACATATTTCCACTTAGAGCAAAAAAAGGAGGAGTC
CTAAAAAGGGCAGGACATACCGAGGCTGCTGTTGACATTGCGGCAATGTCTGGTCTATATCCTGCTGGTGTTATTTGCGA
AATCCAAAATCCGGATGGCTCTATGTCTCGACTGCCTCAACTTAAGGAATATGCTAAAGAATGGGGAATGAAGTTAATAT
CTATTGCAGACCTTATTAGTTATAGATTTCAAAACGAAAGATTTGTTTATAGAAAATCAGAGACAATTCTTCCAAGTATT
TTTGGTAATTTCAAAGCATATGGATACATAAATGATCTTGACGGCTCTGAACATATTGCATTAGTAAAGCAAAAATCAAA
TAAATTAAGTGAACCAGTTTTAGTAAGGATGCATTCAGAATGTTTAACAGGAGATGCTTTTGGCTCACTACGATGTGACT
GCAGACCTCAGCTTGAAGCGGCATTATCTAGGATAGAAAAAGAAGGCGAGGGAGTAGTTGTTTATTTAAGACAAGAAGGA
AGAGGGATCGGTTTGATTAATAAATTAAAAGCATACAGCCTACAAGATGGAGGTTTGGATACTGTGGAAGCAAATGAAAA
ATTAGGTTTTCCCGCTGACCTCAGGAATTATGGAGTTGGAGCTCAAATATTAACTGACTTAGGTATCAAAAAATTAAAAC
TACTAACTAATAATCCTAGGAAAATTGCAGGATTAGGTGGTTATGGAATAGAGGTTACTGAGAGAGTTCCACTAGTCATA
TGTCCTGGAGATCATAATGCTGAATATTTAAATGTAAAAAGACAAAAACTTGGTCACTTATTAGAAGAAGATAAAATAAA
TTCTAGATTTGTGGACCCATATATTGCTATTTTCTTAGATGGTGATTACAAATCGATTGATTTAGTTCCTATCAAAAATA
AAACATTTGAATTCTGTACCAAAAAAAATATAAATATTATGTTAGAGAGTTCTCCAAGATTATTAGCTTTTTGGAATAGA
CCAAAATTAGTATGGAGAATATTACATGATAAAAACAGAGAAGATTTCACAATAAACGATAATGAAATTAAAAATATAGA
AATTTTTATTAAGTTTTTATCTGAATTTGATAAAAGCTCGAAAGTTGGTATTATCGTCTCCAAAAATATCGAGCAGGCGT
TACATCCGAAAAATAACATTAAATTGAAAAATACAAAGTTTTCAATAAAAAATGATTATTTATATTCCTCTACTAGAAAA
TTTAATCTTGATAAAGAAACTTTTAGTATTATATTTAATAAAGATTTAGCTTAG

Upstream 100 bases:

>100_bases
TGCAACATTCATATCTCTTTATTGGCAAACTTTAAGTTAGGATTAATAGTGTTGAAATAAACAATAATTAAAACACTTTT
TTCTATCTTGAATAGTAATA

Downstream 100 bases:

>100_bases
ACTAAGCTAGCTTTAATAATTTTTGATCCGTTTGTAAGTTTTAAAACAATATCCATATCTTCTGTTTTTCCAAAGACAGT
ATGAACTCCGTCTAAATGAG

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II/unknown domain fusion protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKETSPKSNNGTILDNNEKFKIEFDQISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGDKLDALDLPLMVDRNTDKNQTAFTVSIDAGPENNVTTGISAEDRAKTIQVAINPSTKPEDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKEWGMKLISIADLISYRFQNERFVYRKSETILPSI
FGNFKAYGYINDLDGSEHIALVKQKSNKLSEPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEGEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVTERVPLVI
CPGDHNAEYLNVKRQKLGHLLEEDKINSRFVDPYIAIFLDGDYKSIDLVPIKNKTFEFCTKKNINIMLESSPRLLAFWNR
PKLVWRILHDKNREDFTINDNEIKNIEIFIKFLSEFDKSSKVGIIVSKNIEQALHPKNNIKLKNTKFSIKNDYLYSSTRK
FNLDKETFSIIFNKDLA

Sequences:

>Translated_577_residues
MKETSPKSNNGTILDNNEKFKIEFDQISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGDKLDALDLPLMVDRNTDKNQTAFTVSIDAGPENNVTTGISAEDRAKTIQVAINPSTKPEDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKEWGMKLISIADLISYRFQNERFVYRKSETILPSI
FGNFKAYGYINDLDGSEHIALVKQKSNKLSEPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEGEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVTERVPLVI
CPGDHNAEYLNVKRQKLGHLLEEDKINSRFVDPYIAIFLDGDYKSIDLVPIKNKTFEFCTKKNINIMLESSPRLLAFWNR
PKLVWRILHDKNREDFTINDNEIKNIEIFIKFLSEFDKSSKVGIIVSKNIEQALHPKNNIKLKNTKFSIKNDYLYSSTRK
FNLDKETFSIIFNKDLA
>Mature_577_residues
MKETSPKSNNGTILDNNEKFKIEFDQISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGDKLDALDLPLMVDRNTDKNQTAFTVSIDAGPENNVTTGISAEDRAKTIQVAINPSTKPEDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKEWGMKLISIADLISYRFQNERFVYRKSETILPSI
FGNFKAYGYINDLDGSEHIALVKQKSNKLSEPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEGEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVTERVPLVI
CPGDHNAEYLNVKRQKLGHLLEEDKINSRFVDPYIAIFLDGDYKSIDLVPIKNKTFEFCTKKNINIMLESSPRLLAFWNR
PKLVWRILHDKNREDFTINDNEIKNIEIFIKFLSEFDKSSKVGIIVSKNIEQALHPKNNIKLKNTKFSIKNDYLYSSTRK
FNLDKETFSIIFNKDLA

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=218, Percent_Identity=47.2477064220183, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1787533, Length=191, Percent_Identity=50.7853403141361, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=41.747572815534, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319438, Length=172, Percent_Identity=45.9302325581395, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 64726; Mature: 64726

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETSPKSNNGTILDNNEKFKIEFDQISDALAAIRNGECIIVVDDERRENEGDLICAAQF
CCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEC
ATPQQINFMATEGRGLICLAMQGDKLDALDLPLMVDRNTDKNQTAFTVSIDAGPENNVTT
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCEEC
GISAEDRAKTIQVAINPSTKPEDLRRPGHIFPLRAKKGGVLKRAGHTEAAVDIAAMSGLY
CCCCCCCCEEEEEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHEEHHHHCCCC
PAGVICEIQNPDGSMSRLPQLKEYAKEWGMKLISIADLISYRFQNERFVYRKSETILPSI
CCCEEEEEECCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
FGNFKAYGYINDLDGSEHIALVKQKSNKLSEPVLVRMHSECLTGDAFGSLRCDCRPQLEA
HCCCEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCCCCEEECCCHHHHH
ALSRIEKEGEGVVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVG
HHHHHHHCCCCEEEEEEECCCCCCCHHHHEEEEECCCCCCEEECCCCCCCCHHHHHCCCC
AQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVTERVPLVICPGDHNAEYLNVKRQKLGHL
HHHHHHCCCEEEEEEECCCCEEEECCCCCEEEECCCCEEEECCCCCCCEEEEHHHHHHHH
LEEDKINSRFVDPYIAIFLDGDYKSIDLVPIKNKTFEFCTKKNINIMLESSPRLLAFWNR
HHHCCCCCEECCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCEEEEECCCCEEEEECC
PKLVWRILHDKNREDFTINDNEIKNIEIFIKFLSEFDKSSKVGIIVSKNIEQALHPKNNI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCE
KLKNTKFSIKNDYLYSSTRKFNLDKETFSIIFNKDLA
EEECEEEEEECCEEEECCEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MKETSPKSNNGTILDNNEKFKIEFDQISDALAAIRNGECIIVVDDERRENEGDLICAAQF
CCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEC
ATPQQINFMATEGRGLICLAMQGDKLDALDLPLMVDRNTDKNQTAFTVSIDAGPENNVTT
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCEEC
GISAEDRAKTIQVAINPSTKPEDLRRPGHIFPLRAKKGGVLKRAGHTEAAVDIAAMSGLY
CCCCCCCCEEEEEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHEEHHHHCCCC
PAGVICEIQNPDGSMSRLPQLKEYAKEWGMKLISIADLISYRFQNERFVYRKSETILPSI
CCCEEEEEECCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
FGNFKAYGYINDLDGSEHIALVKQKSNKLSEPVLVRMHSECLTGDAFGSLRCDCRPQLEA
HCCCEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCCCCEEECCCHHHHH
ALSRIEKEGEGVVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVG
HHHHHHHCCCCEEEEEEECCCCCCCHHHHEEEEECCCCCCEEECCCCCCCCHHHHHCCCC
AQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVTERVPLVICPGDHNAEYLNVKRQKLGHL
HHHHHHCCCEEEEEEECCCCEEEECCCCCEEEECCCCEEEECCCCCCCEEEEHHHHHHHH
LEEDKINSRFVDPYIAIFLDGDYKSIDLVPIKNKTFEFCTKKNINIMLESSPRLLAFWNR
HHHCCCCCEECCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCEEEEECCCCEEEEECC
PKLVWRILHDKNREDFTINDNEIKNIEIFIKFLSEFDKSSKVGIIVSKNIEQALHPKNNI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCE
KLKNTKFSIKNDYLYSSTRKFNLDKETFSIIFNKDLA
EEECEEEEEECCEEEECCEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]