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The map label for this gene is purN [H]

Identifier: 123966206

GI number: 123966206

Start: 869877

End: 870533

Strand: Direct

Name: purN [H]

Synonym: P9515_09731

Alternate gene names: 123966206

Gene position: 869877-870533 (Clockwise)

Preceding gene: 123966204

Following gene: 123966208

Centisome position: 51.04

GC content: 29.22

Gene sequence:

>657_bases
TTGGATAAGTCATTCAATTATTTAATTTCACCCGAGACTATAAAACTTAGGGGATTTTCACCAAAATTAAAAATTGCAAT
TTTAGCTTCAGGTGAAGGATCAAATTTTCAGGAATTAATTGACCTATCTAAATCTAATAAATTTGATATTGATATTAGAA
TTCTAATTACAAATAAATCAGATGCTGGTTGTATATCAAGAGCAAAAAAATCTAATATTTCCTACAAGATAATTAAAAAA
TCTGACAATGAAAATAACGATTGTTTTGAGGAGGAAATTATCAACACTATAAAAAATTATGATGTAGAACTTATTGTTAT
GGCAGGATGGATGAAAATTATGTCATCGAGATTTGTTAATGTATTTAGGAGTAAAATAATAAATATTCATCCATCCTTAC
TTCCTTCCTTTAAAGGAAATAATGCGATTAAAGAAGCCATAAAACATGATTCCAAGATCACTGGTTGTTCAGTACATTTT
GTAGAACCTGAAGTTGATAGCGGTGACCTAATTATGCAAGCAGCATTACCTATTCTTGATCAAGACAACTTAGAGACCAT
ATCGAAGAAAATACATTTTTTGGAACATAAGATTTTACCACTCTCAATTTCGCAGGCAGGATTTATAGTAAGAAATAGCT
TCAAGGGTAATGATTAA

Upstream 100 bases:

>100_bases
ATATTATTTTTAAATAATAATGTTTGTGATTTTAAATAATTAATATAAAGGGTTTTATTTATTTATTTCTGATTTATTAT
TCAATTAAAAATATCAGATT

Downstream 100 bases:

>100_bases
TCATTTCTAATCCATTATTTAATGGTAAACCAGATATAAGATTAAGATTTTGTATAGCTTGACCTGTTTGTCCTTTAAGT
AAATTATCTATTACTGATAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDKSFNYLISPETIKLRGFSPKLKIAILASGEGSNFQELIDLSKSNKFDIDIRILITNKSDAGCISRAKKSNISYKIIKK
SDNENNDCFEEEIINTIKNYDVELIVMAGWMKIMSSRFVNVFRSKIINIHPSLLPSFKGNNAIKEAIKHDSKITGCSVHF
VEPEVDSGDLIMQAALPILDQDNLETISKKIHFLEHKILPLSISQAGFIVRNSFKGND

Sequences:

>Translated_218_residues
MDKSFNYLISPETIKLRGFSPKLKIAILASGEGSNFQELIDLSKSNKFDIDIRILITNKSDAGCISRAKKSNISYKIIKK
SDNENNDCFEEEIINTIKNYDVELIVMAGWMKIMSSRFVNVFRSKIINIHPSLLPSFKGNNAIKEAIKHDSKITGCSVHF
VEPEVDSGDLIMQAALPILDQDNLETISKKIHFLEHKILPLSISQAGFIVRNSFKGND
>Mature_218_residues
MDKSFNYLISPETIKLRGFSPKLKIAILASGEGSNFQELIDLSKSNKFDIDIRILITNKSDAGCISRAKKSNISYKIIKK
SDNENNDCFEEEIINTIKNYDVELIVMAGWMKIMSSRFVNVFRSKIINIHPSLLPSFKGNNAIKEAIKHDSKITGCSVHF
VEPEVDSGDLIMQAALPILDQDNLETISKKIHFLEHKILPLSISQAGFIVRNSFKGND

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=37.3626373626374, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=37.3626373626374, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=37.3626373626374, Blast_Score=150, Evalue=7e-37,
Organism=Escherichia coli, GI1788846, Length=182, Percent_Identity=36.2637362637363, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1787483, Length=160, Percent_Identity=27.5, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=37.4301675977654, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=30.6122448979592, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582400, Length=183, Percent_Identity=36.6120218579235, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24520; Mature: 24520

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSFNYLISPETIKLRGFSPKLKIAILASGEGSNFQELIDLSKSNKFDIDIRILITNKS
CCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEEEEEECCC
DAGCISRAKKSNISYKIIKKSDNENNDCFEEEIINTIKNYDVELIVMAGWMKIMSSRFVN
CCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHH
VFRSKIINIHPSLLPSFKGNNAIKEAIKHDSKITGCSVHFVEPEVDSGDLIMQAALPILD
HHHHHHEECCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEHHCCCCC
QDNLETISKKIHFLEHKILPLSISQAGFIVRNSFKGND
CCCHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MDKSFNYLISPETIKLRGFSPKLKIAILASGEGSNFQELIDLSKSNKFDIDIRILITNKS
CCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEEEEEECCC
DAGCISRAKKSNISYKIIKKSDNENNDCFEEEIINTIKNYDVELIVMAGWMKIMSSRFVN
CCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHH
VFRSKIINIHPSLLPSFKGNNAIKEAIKHDSKITGCSVHFVEPEVDSGDLIMQAALPILD
HHHHHHEECCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEHHCCCCC
QDNLETISKKIHFLEHKILPLSISQAGFIVRNSFKGND
CCCHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]