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The map label for this gene is ispG [H]

Identifier: 123965984

GI number: 123965984

Start: 677928

End: 679145

Strand: Direct

Name: ispG [H]

Synonym: P9515_07491

Alternate gene names: 123965984

Gene position: 677928-679145 (Clockwise)

Preceding gene: 123965983

Following gene: 123965985

Centisome position: 39.78

GC content: 35.22

Gene sequence:

>1218_bases
ATGTCATTAACTCAATCAAAAGAGGTGAATAGTCTCTCAAGAAGATATTCAACTCATATTGAGAGAAGGATTACTAGAAC
TGTAATGGTTGGAGATATTGCAATTGGTAGTGACTATCCAGTAAGAGTTCAGTCAATGATAAATGAAGATACTATGGATG
TGGATAATGCTTACTTAGCCATTAAAAGACTTCACGATGTGGGCTGTGAAATCGTAAGACTTACTGTCCCTTCTCTAGCT
CATGCAAAAGCTGTTGGAGATATTAAAGAAAAATTAATTAAAAGTAATATAAATACACCACTAGTCGCAGATGTTCATCA
CAATGGAATGAAAATTGCAATGGAGGTTGCAAAACATGTTGATAAAGTAAGGATTAATCCAGGATTGTTTGTTTTTGAGA
AATCAGATCCTACAAGAACTAAATATACTGATGCAGAATTTGAGACTATTAAAAATACAATCCTAAAAAGATTTACTCCA
CTTGTAGAAGTTTTGAAGTCGAAAAATAAAGCTCTAAGGATCGGAGTTAATCATGGTTCTTTATCCGAGAGAATGCTTTT
TACTTATGGGGATACACCTTTAGGAATGACAGAATCTGCAATGGAATTTGTAAAAATTTGCGATGAACTTGATTTTCATA
ATATTATTATTTCTATGAAGGCTTCAAGAGCACCAGTAATGTTGGCAGCATATAGGATGATTTCAGATAGATTGGATGCA
GAAGGATATAACTACCCTTTGCATTTAGGCGTCACTGAAGCTGGAGATGGAGATTATGGAAGAATTAAGAGTACTGCAGG
TATAGGAACACTCCTTGCAGAGGGTCTTGGAGATACTATAAGAGTCTCATTAACTGAAGCTCCTGAAAAGGAAATTCCAG
TATGCTATTCAATTTTGCAATCCTTAGGATTAAGAAAAACAATGGTTGAATATATAAGTTGTCCTAGTTGCGGAAGAACT
CTTTTTAATCTCGAGGAAGTTGTAGATAAAGTTAGGAATGCTACCTCTCATTTAACTGGTTTAGATATTGCAATAATGGG
TTGTATTGTGAACGGACCTGGAGAAATGGCAGATGCTGATTATGGCTACGTAGGGAAAGGAAAAGGGACAATTGCTCTTT
ACAGAAGGAAAGAAGAGATAAAAAGAGTACCTGAGGATGAGGGGGTTGATGCTTTAATTAGACTTATTAAAGAAGATGGG
AAATGGATTGATCCTTAA

Upstream 100 bases:

>100_bases
GGGAAACCATATGATCTAACTTTAAAAGACCTTAAGAATGTTAGTAGGAAACTATATGCTTTATAATTTAAAGAAGACTT
TTTAGAATTTCTAGAATTTA

Downstream 100 bases:

>100_bases
ATAAATTTTTAATTTATAATTCGATAAATAGAAATTTTAATTTTGAATACTACTTCCAAGCCTTTAAAGATAAAAAAATT
ATTTAAAAAGCAGATTGTGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSLTQSKEVNSLSRRYSTHIERRITRTVMVGDIAIGSDYPVRVQSMINEDTMDVDNAYLAIKRLHDVGCEIVRLTVPSLA
HAKAVGDIKEKLIKSNINTPLVADVHHNGMKIAMEVAKHVDKVRINPGLFVFEKSDPTRTKYTDAEFETIKNTILKRFTP
LVEVLKSKNKALRIGVNHGSLSERMLFTYGDTPLGMTESAMEFVKICDELDFHNIIISMKASRAPVMLAAYRMISDRLDA
EGYNYPLHLGVTEAGDGDYGRIKSTAGIGTLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSLGLRKTMVEYISCPSCGRT
LFNLEEVVDKVRNATSHLTGLDIAIMGCIVNGPGEMADADYGYVGKGKGTIALYRRKEEIKRVPEDEGVDALIRLIKEDG
KWIDP

Sequences:

>Translated_405_residues
MSLTQSKEVNSLSRRYSTHIERRITRTVMVGDIAIGSDYPVRVQSMINEDTMDVDNAYLAIKRLHDVGCEIVRLTVPSLA
HAKAVGDIKEKLIKSNINTPLVADVHHNGMKIAMEVAKHVDKVRINPGLFVFEKSDPTRTKYTDAEFETIKNTILKRFTP
LVEVLKSKNKALRIGVNHGSLSERMLFTYGDTPLGMTESAMEFVKICDELDFHNIIISMKASRAPVMLAAYRMISDRLDA
EGYNYPLHLGVTEAGDGDYGRIKSTAGIGTLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSLGLRKTMVEYISCPSCGRT
LFNLEEVVDKVRNATSHLTGLDIAIMGCIVNGPGEMADADYGYVGKGKGTIALYRRKEEIKRVPEDEGVDALIRLIKEDG
KWIDP
>Mature_404_residues
SLTQSKEVNSLSRRYSTHIERRITRTVMVGDIAIGSDYPVRVQSMINEDTMDVDNAYLAIKRLHDVGCEIVRLTVPSLAH
AKAVGDIKEKLIKSNINTPLVADVHHNGMKIAMEVAKHVDKVRINPGLFVFEKSDPTRTKYTDAEFETIKNTILKRFTPL
VEVLKSKNKALRIGVNHGSLSERMLFTYGDTPLGMTESAMEFVKICDELDFHNIIISMKASRAPVMLAAYRMISDRLDAE
GYNYPLHLGVTEAGDGDYGRIKSTAGIGTLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSLGLRKTMVEYISCPSCGRTL
FNLEEVVDKVRNATSHLTGLDIAIMGCIVNGPGEMADADYGYVGKGKGTIALYRRKEEIKRVPEDEGVDALIRLIKEDGK
WIDP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=388, Percent_Identity=37.3711340206186, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44909; Mature: 44778

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTQSKEVNSLSRRYSTHIERRITRTVMVGDIAIGSDYPVRVQSMINEDTMDVDNAYLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
IKRLHDVGCEIVRLTVPSLAHAKAVGDIKEKLIKSNINTPLVADVHHNGMKIAMEVAKHV
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
DKVRINPGLFVFEKSDPTRTKYTDAEFETIKNTILKRFTPLVEVLKSKNKALRIGVNHGS
HHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LSERMLFTYGDTPLGMTESAMEFVKICDELDFHNIIISMKASRAPVMLAAYRMISDRLDA
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
EGYNYPLHLGVTEAGDGDYGRIKSTAGIGTLLAEGLGDTIRVSLTEAPEKEIPVCYSILQ
CCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
SLGLRKTMVEYISCPSCGRTLFNLEEVVDKVRNATSHLTGLDIAIMGCIVNGPGEMADAD
HHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC
YGYVGKGKGTIALYRRKEEIKRVPEDEGVDALIRLIKEDGKWIDP
CCEEECCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLTQSKEVNSLSRRYSTHIERRITRTVMVGDIAIGSDYPVRVQSMINEDTMDVDNAYLA
CCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
IKRLHDVGCEIVRLTVPSLAHAKAVGDIKEKLIKSNINTPLVADVHHNGMKIAMEVAKHV
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
DKVRINPGLFVFEKSDPTRTKYTDAEFETIKNTILKRFTPLVEVLKSKNKALRIGVNHGS
HHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LSERMLFTYGDTPLGMTESAMEFVKICDELDFHNIIISMKASRAPVMLAAYRMISDRLDA
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
EGYNYPLHLGVTEAGDGDYGRIKSTAGIGTLLAEGLGDTIRVSLTEAPEKEIPVCYSILQ
CCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
SLGLRKTMVEYISCPSCGRTLFNLEEVVDKVRNATSHLTGLDIAIMGCIVNGPGEMADAD
HHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC
YGYVGKGKGTIALYRRKEEIKRVPEDEGVDALIRLIKEDGKWIDP
CCEEECCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]