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The map label for this gene is rsmE [H]

Identifier: 123965976

GI number: 123965976

Start: 670599

End: 671378

Strand: Direct

Name: rsmE [H]

Synonym: P9515_07411

Alternate gene names: 123965976

Gene position: 670599-671378 (Clockwise)

Preceding gene: 123965975

Following gene: 123965977

Centisome position: 39.35

GC content: 26.15

Gene sequence:

>780_bases
ATGGATGATTTAATAAGATTACTTATTCAAAATGAAAGAATAATTGGTAATAAAAACAAGAATTTAAAACTTACAAAAGA
AGAAGCTCACTATTTAAATAAAGTAATGCGTATAAAAATTGGTAGAGAAATTTTTATAACTAATGGTCAAGGTTCTCTAT
GGAAAGCTAAGAAATTGGAACAAAACTTTTTAGAGATAAATAATATTGATAATCCATATTTTGTTCAAGAAAAAGAAAAA
GTTTCTTTAGGAATAGCTGTTGTTGTTCCAAAGAATGGCTTTGAGGATATTCTTAAGATGTGTACTGAAATAGGAATTGA
TTTAATACAACCTTTATTTTCAGAAAGACAAGTTAAAAATAACTCTAACTTTTTAAAAAGAAATATAAGATGGAATTCAA
TAATCAATGAAGCGGTTGAACAAAGTGAAAGGTTATGGAGACCACTCATCTTAGGTGGAGTTAATTTAATAGATTGGATA
GATTCAAGGGATTCCAAAGATATAATTTCAATCTCTATTACAAGAGATGATAGTTCTGAAAATTTAAATCATTGGTTAAA
AAGAAAGCAAAATCTTTTGAAAAAAAATGGTGGAGTTTTATGGAATGTCGTTGGACCTGAGGGAGGTTGGTCAAGAAATG
AAATTGAATTTTTTTTAAAAAATAAAATTTCTTTTGTCAAACTTTCAGAAACTATTTTGAGAACTTCAACAGCTACTGTT
AATGCAACTTCAATTCTTAATCAATGGCGAAATGATTTAAAATTAAATAATTATTTATAA

Upstream 100 bases:

>100_bases
TAATTATGTAAAAATTGATCAATTAATTATTGGAGGAGAAAATAAGGATTGGTCAATTGAGGAACATGAAGATTTAGTTT
TTAAAAATTGATATTCATAT

Downstream 100 bases:

>100_bases
ATTATGAGTTTAATTTCTAATAGTTTTGTATTGGGTTTCTGCATAAATTTTAGTTTAATTCTTTTGTTTTATAGGTTCCC
ATTGATGACAAAAGGTGGAT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDDLIRLLIQNERIIGNKNKNLKLTKEEAHYLNKVMRIKIGREIFITNGQGSLWKAKKLEQNFLEINNIDNPYFVQEKEK
VSLGIAVVVPKNGFEDILKMCTEIGIDLIQPLFSERQVKNNSNFLKRNIRWNSIINEAVEQSERLWRPLILGGVNLIDWI
DSRDSKDIISISITRDDSSENLNHWLKRKQNLLKKNGGVLWNVVGPEGGWSRNEIEFFLKNKISFVKLSETILRTSTATV
NATSILNQWRNDLKLNNYL

Sequences:

>Translated_259_residues
MDDLIRLLIQNERIIGNKNKNLKLTKEEAHYLNKVMRIKIGREIFITNGQGSLWKAKKLEQNFLEINNIDNPYFVQEKEK
VSLGIAVVVPKNGFEDILKMCTEIGIDLIQPLFSERQVKNNSNFLKRNIRWNSIINEAVEQSERLWRPLILGGVNLIDWI
DSRDSKDIISISITRDDSSENLNHWLKRKQNLLKKNGGVLWNVVGPEGGWSRNEIEFFLKNKISFVKLSETILRTSTATV
NATSILNQWRNDLKLNNYL
>Mature_259_residues
MDDLIRLLIQNERIIGNKNKNLKLTKEEAHYLNKVMRIKIGREIFITNGQGSLWKAKKLEQNFLEINNIDNPYFVQEKEK
VSLGIAVVVPKNGFEDILKMCTEIGIDLIQPLFSERQVKNNSNFLKRNIRWNSIINEAVEQSERLWRPLILGGVNLIDWI
DSRDSKDIISISITRDDSSENLNHWLKRKQNLLKKNGGVLWNVVGPEGGWSRNEIEFFLKNKISFVKLSETILRTSTATV
NATSILNQWRNDLKLNNYL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=248, Percent_Identity=25, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 30088; Mature: 30088

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLIRLLIQNERIIGNKNKNLKLTKEEAHYLNKVMRIKIGREIFITNGQGSLWKAKKLE
CHHHHHHHHHCCEEECCCCCCEEEEHHHHHHHHHHHHEEECCEEEEECCCCCCHHHHHHH
QNFLEINNIDNPYFVQEKEKVSLGIAVVVPKNGFEDILKMCTEIGIDLIQPLFSERQVKN
HHHHHHCCCCCCEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHCC
NSNFLKRNIRWNSIINEAVEQSERLWRPLILGGVNLIDWIDSRDSKDIISISITRDDSSE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCHH
NLNHWLKRKQNLLKKNGGVLWNVVGPEGGWSRNEIEFFLKNKISFVKLSETILRTSTATV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCCCC
NATSILNQWRNDLKLNNYL
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDDLIRLLIQNERIIGNKNKNLKLTKEEAHYLNKVMRIKIGREIFITNGQGSLWKAKKLE
CHHHHHHHHHCCEEECCCCCCEEEEHHHHHHHHHHHHEEECCEEEEECCCCCCHHHHHHH
QNFLEINNIDNPYFVQEKEKVSLGIAVVVPKNGFEDILKMCTEIGIDLIQPLFSERQVKN
HHHHHHCCCCCCEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHCC
NSNFLKRNIRWNSIINEAVEQSERLWRPLILGGVNLIDWIDSRDSKDIISISITRDDSSE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCHH
NLNHWLKRKQNLLKKNGGVLWNVVGPEGGWSRNEIEFFLKNKISFVKLSETILRTSTATV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCCCC
NATSILNQWRNDLKLNNYL
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]