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The map label for this gene is ilvB [H]

Identifier: 123965825

GI number: 123965825

Start: 528016

End: 529779

Strand: Direct

Name: ilvB [H]

Synonym: P9515_05901

Alternate gene names: 123965825

Gene position: 528016-529779 (Clockwise)

Preceding gene: 123965824

Following gene: 123965826

Centisome position: 30.98

GC content: 37.47

Gene sequence:

>1764_bases
GTGACTCTTACTTCTCCACCTTTGTCAAAAGGTGATTCAGAAAAAAAATACCCAAAATGGATTACAGGTGCAGAAGCACT
TATGGATTCACTAAGAATCCATGGTGTGAGAGTTATATTTGGATATCCCGGAGGAGCTATACTCCCAATTTATGATGCTG
TATATAAGGCAGAGAGTGATGGATGGTTGAAACACTATATGGTTAGACATGAGCAGGGAGGTTCCCATGCCGCAGATGGG
TATGCGAGAGCAACTGGTGAGGTAGGAGTATGTTTTGGAACTTCAGGCCCAGGAGCTACGAATTTAGTAACGGGAATTGC
AACAGCTCAGATGGATTCAGTCCCATTGGTGGTGGTCACTGGCCAAGTTCCAAGACCTGCTATCGGAACAGATGCTTTTC
AAGAGACTGATATTTTTGGGATAACACTTCCTATCGTTAAACATTCATGGGTAATTAGAGATCCTTCAGATATAGCGAAA
GTAGTTTCAGAAGCTTTTTTCATTGCATCCTCAGGAAGACCCGGCCCAGTTTTAATTGACATACCAAAAGATGTGGGTCA
AGAATTCTTTAATTATGAAAGAATTTTACCAGGTGAAATTATTCCTAAAGGTTTTAAAAGGAATGGTGAAATTAATGATA
ATGATATCGATAAAGCAATTGAATTAATTAAACAGTCTGCAAAACCTTTGCTCTATGTGGGTGGTGGTGCTATATCTTCT
GGGGCTCATAAGGAAGTGGAAATACTAGCAAAGAATTTTCAAATTCCTGTCACAACTACTTTAATGGGGAAGGGAGCTTT
TGATGAAAGGGATGATTTATCTGTGGGAATGTTGGGTATGCATGGAACTGCATATGCAAATTTTGCAGTAACTGAATGCG
ATCTTTTGATTACTGTTGGAGCGAGATTTGATGATAGAGTCACTGGCAAATTAGATACATTTGCTCCATCTGCAAAAGTT
ATTCATATCGATATTGATCCCGCAGAAGTTAATAAAAATAGACGTGTTGATGTTGCAATCGTTTCTGATGTGGCTGTAGC
TCTTTCAAAAATCAATGAAAAATCTTTAAGTAAAAATACTTCGTGTAATACTAGAAATTGGTTAGAAAAAATAAATTCTT
GGAAAAATAAACATCCTTTATTTATTCCTCCTGAAGAAGGAGAAATTTACCCTCAAGAAGTACTTTTAAAAGTTAGAGAT
TTGTCCCCTGAAGCTTTCATTACAACAGATGTAGGTCAACATCAAATGTGGGCTGCGCAGTATCTCAGAAATGCCCCAAG
AAGGTGGATTAGTAGTGCAGGCTTAGGAACAATGGGCTTTGGATTACCTGCCGCAATGGGAGTAAAAGCTGCATTACCAA
CATCTGAAGTTATTTGTATAGCTGGTGATGCAAGTGTTTTGATGAATATACAAGAATTGGGTACTTTATCTCAATATGGT
CTAAAAATTAAATTAGTTATAATAAATAATCGCTGGCAAGGAATGGTTAGACAATGGCAAGAAAGCTTTTATCATGAAAG
GTATTCTTCTTCCGATATGAGTTGTGGAGAACCAGATTTCGTTAAGCTAGCAGAATCCTTTGGAGTTAAGGGATTTTTAA
TCTCCGAGAGAAAACAATTACATAATGAATTTAAGTCTGCTTTAGATTTTGATGGTCCAGCCTTAATTAATGTTCGAGTT
AGGAGGGGAGAAAATTGTTATCCAATGGTTCCCCCAGGTAAAAGTAATGCTCAAATGGTTGGTTACGTTAACTGTAAAAA
CTAA

Upstream 100 bases:

>100_bases
TTAAAAATATTTAATTTAGAAGTGGTTCCTGACATTACGTTTCTAAATTAGGCAAAATTTTTTAATTAAGTTTAAGATTT
ATATAGAATTATTTTTCTTA

Downstream 100 bases:

>100_bases
TTATTGTTTTTGGATAAAATTAACAACTTATAATAAAAATAAATTATTGAGTTTACCTTAATTGAAAAAAGTTATATCGA
TATTAATAATTTTTGTTTTG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTLTSPPLSKGDSEKKYPKWITGAEALMDSLRIHGVRVIFGYPGGAILPIYDAVYKAESDGWLKHYMVRHEQGGSHAADG
YARATGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFNYERILPGEIIPKGFKRNGEINDNDIDKAIELIKQSAKPLLYVGGGAISS
GAHKEVEILAKNFQIPVTTTLMGKGAFDERDDLSVGMLGMHGTAYANFAVTECDLLITVGARFDDRVTGKLDTFAPSAKV
IHIDIDPAEVNKNRRVDVAIVSDVAVALSKINEKSLSKNTSCNTRNWLEKINSWKNKHPLFIPPEEGEIYPQEVLLKVRD
LSPEAFITTDVGQHQMWAAQYLRNAPRRWISSAGLGTMGFGLPAAMGVKAALPTSEVICIAGDASVLMNIQELGTLSQYG
LKIKLVIINNRWQGMVRQWQESFYHERYSSSDMSCGEPDFVKLAESFGVKGFLISERKQLHNEFKSALDFDGPALINVRV
RRGENCYPMVPPGKSNAQMVGYVNCKN

Sequences:

>Translated_587_residues
MTLTSPPLSKGDSEKKYPKWITGAEALMDSLRIHGVRVIFGYPGGAILPIYDAVYKAESDGWLKHYMVRHEQGGSHAADG
YARATGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFNYERILPGEIIPKGFKRNGEINDNDIDKAIELIKQSAKPLLYVGGGAISS
GAHKEVEILAKNFQIPVTTTLMGKGAFDERDDLSVGMLGMHGTAYANFAVTECDLLITVGARFDDRVTGKLDTFAPSAKV
IHIDIDPAEVNKNRRVDVAIVSDVAVALSKINEKSLSKNTSCNTRNWLEKINSWKNKHPLFIPPEEGEIYPQEVLLKVRD
LSPEAFITTDVGQHQMWAAQYLRNAPRRWISSAGLGTMGFGLPAAMGVKAALPTSEVICIAGDASVLMNIQELGTLSQYG
LKIKLVIINNRWQGMVRQWQESFYHERYSSSDMSCGEPDFVKLAESFGVKGFLISERKQLHNEFKSALDFDGPALINVRV
RRGENCYPMVPPGKSNAQMVGYVNCKN
>Mature_586_residues
TLTSPPLSKGDSEKKYPKWITGAEALMDSLRIHGVRVIFGYPGGAILPIYDAVYKAESDGWLKHYMVRHEQGGSHAADGY
ARATGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKV
VSEAFFIASSGRPGPVLIDIPKDVGQEFFNYERILPGEIIPKGFKRNGEINDNDIDKAIELIKQSAKPLLYVGGGAISSG
AHKEVEILAKNFQIPVTTTLMGKGAFDERDDLSVGMLGMHGTAYANFAVTECDLLITVGARFDDRVTGKLDTFAPSAKVI
HIDIDPAEVNKNRRVDVAIVSDVAVALSKINEKSLSKNTSCNTRNWLEKINSWKNKHPLFIPPEEGEIYPQEVLLKVRDL
SPEAFITTDVGQHQMWAAQYLRNAPRRWISSAGLGTMGFGLPAAMGVKAALPTSEVICIAGDASVLMNIQELGTLSQYGL
KIKLVIINNRWQGMVRQWQESFYHERYSSSDMSCGEPDFVKLAESFGVKGFLISERKQLHNEFKSALDFDGPALINVRVR
RGENCYPMVPPGKSNAQMVGYVNCKN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=25.2189141856392, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=24.3243243243243, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=44.9820788530466, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=42.3008849557522, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=31.7543859649123, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=26.3913824057451, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=23.8351254480287, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=604, Percent_Identity=26.158940397351, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=25.4385964912281, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=25.3097345132743, Blast_Score=128, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=42.320819112628, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=572, Percent_Identity=23.0769230769231, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=511, Percent_Identity=24.26614481409, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922626, Length=573, Percent_Identity=25.3054101221641, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64133; Mature: 64002

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTSPPLSKGDSEKKYPKWITGAEALMDSLRIHGVRVIFGYPGGAILPIYDAVYKAESD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCEEHHHHHHHHCCCC
GWLKHYMVRHEQGGSHAADGYARATGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVT
CHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCEEEEECHHHHHHHHHHHEEEECCCCCCCEEEE
IPKDVGQEFFNYERILPGEIIPKGFKRNGEINDNDIDKAIELIKQSAKPLLYVGGGAISS
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
GAHKEVEILAKNFQIPVTTTLMGKGAFDERDDLSVGMLGMHGTAYANFAVTECDLLITVG
CCCHHHHEEHHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEEEEEEC
ARFDDRVTGKLDTFAPSAKVIHIDIDPAEVNKNRRVDVAIVSDVAVALSKINEKSLSKNT
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCC
SCNTRNWLEKINSWKNKHPLFIPPEEGEIYPQEVLLKVRDLSPEAFITTDVGQHQMWAAQ
CCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
YLRNAPRRWISSAGLGTMGFGLPAAMGVKAALPTSEVICIAGDASVLMNIQELGTLSQYG
HHHHCHHHHHHHCCCCCCCCCCHHHHCCEECCCCCCEEEEECCHHHHHHHHHHCCHHHCC
LKIKLVIINNRWQGMVRQWQESFYHERYSSSDMSCGEPDFVKLAESFGVKGFLISERKQL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHH
HNEFKSALDFDGPALINVRVRRGENCYPMVPPGKSNAQMVGYVNCKN
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TLTSPPLSKGDSEKKYPKWITGAEALMDSLRIHGVRVIFGYPGGAILPIYDAVYKAESD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCEEHHHHHHHHCCCC
GWLKHYMVRHEQGGSHAADGYARATGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVT
CHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCEEEEECHHHHHHHHHHHEEEECCCCCCCEEEE
IPKDVGQEFFNYERILPGEIIPKGFKRNGEINDNDIDKAIELIKQSAKPLLYVGGGAISS
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
GAHKEVEILAKNFQIPVTTTLMGKGAFDERDDLSVGMLGMHGTAYANFAVTECDLLITVG
CCCHHHHEEHHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEEEEEEC
ARFDDRVTGKLDTFAPSAKVIHIDIDPAEVNKNRRVDVAIVSDVAVALSKINEKSLSKNT
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCC
SCNTRNWLEKINSWKNKHPLFIPPEEGEIYPQEVLLKVRDLSPEAFITTDVGQHQMWAAQ
CCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
YLRNAPRRWISSAGLGTMGFGLPAAMGVKAALPTSEVICIAGDASVLMNIQELGTLSQYG
HHHHCHHHHHHHCCCCCCCCCCHHHHCCEECCCCCCEEEEECCHHHHHHHHHHCCHHHCC
LKIKLVIINNRWQGMVRQWQESFYHERYSSSDMSCGEPDFVKLAESFGVKGFLISERKQL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHH
HNEFKSALDFDGPALINVRVRRGENCYPMVPPGKSNAQMVGYVNCKN
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]