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The map label for this gene is rpoD [H]

Identifier: 123965795

GI number: 123965795

Start: 499452

End: 500645

Strand: Reverse

Name: rpoD [H]

Synonym: P9515_05601

Alternate gene names: 123965795

Gene position: 500645-499452 (Counterclockwise)

Preceding gene: 123965797

Following gene: 123965794

Centisome position: 29.38

GC content: 34.76

Gene sequence:

>1194_bases
ATGTGTCCAGTTGCAGCAAAATCAAAAAACTCTAGTCCTAGCTCTAAGAAAAAAACTAATAAAAAAATTAATTCAAATTT
AAAAACCGGTCAAGAAGAAGAGAAAATTACTACAAATCTAGATCCTGTTAAGAACTCGCAACAAAATTCTTTGAAGGATA
AAGAAATTATCGAGAATAATGAAGAATTTAGTGGTTCTGATGAAGAGGCTAAAGCTCTTGGGAACATAAAGCTAGGACCA
AAAGGTATTTATACAGAGGATTCTATAAGAGTTTATCTTCAGGAAATTGGAAGAATAAGACTATTAAGACCCGATGAAGA
AATTGAACTTGCTAGAAAAATAGCTGATCTACTCCAACTTGAAGAGTTAGCTACACAATTCGAGTCTGAGAAGGGACATT
TCCCTTCAGTCAGAGAATGGGCTGAATTAATAGACATGCCTCTTTCAAAATTCAGAAGAAGACTTCTCTTAGGTAGAAGA
GCTAAAGAGAAGATGGTTCAATCTAATTTAAGATTAGTTGTCTCCATAGCTAAGAAATACATGAATAGAGGGTTATCTTT
TCAAGATCTAATTCAAGAAGGAAGCTTAGGTTTAATAAGGGCTGCAGAAAAATTTGATCATGAAAAAGGTTATAAATTCT
CTACTTATGCAACTTGGTGGATTCGTCAAGCGATAACGAGAGCCATAGCAGATCAAAGTAGAACTATCAGATTGCCTGTT
CACTTATATGAGACGATTTCTAGGATAAAAAAAACAACAAAAGTTCTTAGTCAAGAATTCGGAAGAAAACCTAGTGAAGA
AGAGATAGCTGAGAGTATGGAGATGACAATTGAGAAATTAAGATTTATAGCTAAAAGTGCTCAGCTTCCAATTTCTCTAG
AGACTCCTATTGGTAAAGAAGAGGATTCAAGGCTTGGAGACTTTATTGAGGCTGATATTGAGAACCCAGAACAAGATGTA
TCCAAAACCTTATTGAGAGAAGATTTGGAGGGAGTTTTAGCGACTCTTAGTCCAAGAGAGAGGGATGTCCTTAGATTGCG
ATATGGAATTGATGACGGAAGAATGAAAACTCTTGAAGAGATAGGGCAAATATTTGATGTTACAAGGGAAAGAATTAGAC
AGATAGAAGCAAAAGCACTGAGAAAGCTAAGACATCCAAACAGAAATGGGGTTTTAAAGGAATATATTAAATAA

Upstream 100 bases:

>100_bases
GTTCTTCTAAGTCCTTAAGGCAAATTTTTACTACTAATTGTATAAAATTGCCCTAATTAAAATTTTACCTAGTTAAATTC
ACCGTAGAAAGGAGTCCATT

Downstream 100 bases:

>100_bases
GAAAGTTAAATATAATTTTCAGCTTTGTAAAGTTGCCAAAAATTATCTTAAGATAAATTCGACTTTATCTTAAACCTATT
TAAACTTATCATTTAATGAC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MCPVAAKSKNSSPSSKKKTNKKINSNLKTGQEEEKITTNLDPVKNSQQNSLKDKEIIENNEEFSGSDEEAKALGNIKLGP
KGIYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLQLEELATQFESEKGHFPSVREWAELIDMPLSKFRRRLLLGRR
AKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPV
HLYETISRIKKTTKVLSQEFGRKPSEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENPEQDV
SKTLLREDLEGVLATLSPRERDVLRLRYGIDDGRMKTLEEIGQIFDVTRERIRQIEAKALRKLRHPNRNGVLKEYIK

Sequences:

>Translated_397_residues
MCPVAAKSKNSSPSSKKKTNKKINSNLKTGQEEEKITTNLDPVKNSQQNSLKDKEIIENNEEFSGSDEEAKALGNIKLGP
KGIYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLQLEELATQFESEKGHFPSVREWAELIDMPLSKFRRRLLLGRR
AKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPV
HLYETISRIKKTTKVLSQEFGRKPSEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENPEQDV
SKTLLREDLEGVLATLSPRERDVLRLRYGIDDGRMKTLEEIGQIFDVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
>Mature_397_residues
MCPVAAKSKNSSPSSKKKTNKKINSNLKTGQEEEKITTNLDPVKNSQQNSLKDKEIIENNEEFSGSDEEAKALGNIKLGP
KGIYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLQLEELATQFESEKGHFPSVREWAELIDMPLSKFRRRLLLGRR
AKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPV
HLYETISRIKKTTKVLSQEFGRKPSEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENPEQDV
SKTLLREDLEGVLATLSPRERDVLRLRYGIDDGRMKTLEEIGQIFDVTRERIRQIEAKALRKLRHPNRNGVLKEYIK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=59.0361445783133, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789098, Length=236, Percent_Identity=43.6440677966102, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=28.8065843621399, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=29.6116504854369, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45710; Mature: 45710

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPVAAKSKNSSPSSKKKTNKKINSNLKTGQEEEKITTNLDPVKNSQQNSLKDKEIIENN
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHCCCHHHHHCCC
EEFSGSDEEAKALGNIKLGPKGIYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLQL
CCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
EELATQFESEKGHFPSVREWAELIDMPLSKFRRRLLLGRRAKEKMVQSNLRLVVSIAKKY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPV
HHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHH
HLYETISRIKKTTKVLSQEFGRKPSEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
EDSRLGDFIEADIENPEQDVSKTLLREDLEGVLATLSPRERDVLRLRYGIDDGRMKTLEE
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
IGQIFDVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure
MCPVAAKSKNSSPSSKKKTNKKINSNLKTGQEEEKITTNLDPVKNSQQNSLKDKEIIENN
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHCCCHHHHHCCC
EEFSGSDEEAKALGNIKLGPKGIYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLQL
CCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
EELATQFESEKGHFPSVREWAELIDMPLSKFRRRLLLGRRAKEKMVQSNLRLVVSIAKKY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPV
HHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHH
HLYETISRIKKTTKVLSQEFGRKPSEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
EDSRLGDFIEADIENPEQDVSKTLLREDLEGVLATLSPRERDVLRLRYGIDDGRMKTLEE
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
IGQIFDVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]