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The map label for this gene is ndhB [H]

Identifier: 123965732

GI number: 123965732

Start: 443429

End: 444949

Strand: Reverse

Name: ndhB [H]

Synonym: P9515_04971

Alternate gene names: 123965732

Gene position: 444949-443429 (Counterclockwise)

Preceding gene: 123965738

Following gene: 123965731

Centisome position: 26.11

GC content: 35.9

Gene sequence:

>1521_bases
GTGCCTAACGAAATCTTTACAATTAATTTAAATGCTCAAGCAATTATTCCAGAAGCTTTCATTTTGCTTGGTATTGTTGG
TACTCTTCTTGTTGATCTAGCTGGCGAAAAGACAGCCTCTAGGTGGGCGCCTGTGATTTGTTATATTTCTTTAGGCAGTT
CTCTGGTAAGTCTCGCTTTTCAGTGGAGTAACCCTGTTAATAACGCATTCCTTGGTTCTTTTAATTCAGATAATCTAGCT
ATTGCATTTAGATCAATCATTGCATTATCAACTTTGATTTCCTTACTCATTAGTTGGCGTTATACAGAGCAGAGTGGAAG
TCCAATCGGAGAATTTGCTGCAATAGTTTTATCGGCAACTTTAGGAGCCATGCTTTTGTGTGGATCTACTGACCTTGTAA
GTGTATTTATATCTTTAGAAACTTTATCAGTAGCAAGTTATTTGCTTTCCGGTTACCTCAAAAGAGACCCAAGAAGCTCA
GAGGCCGCTTTAAAATATCTTCTTGTCGGATCAGCAGCAGCTGCAATTTATTTATATGGATCCTCATTTCTTTATGGTCT
AAGTGGTTCCACAAACTTATCAACTATTGGAGTAGAGATAATAAATAAGCCATCTTTTATTACATCCTTATCTCTAGTCT
TTGTCTTATCAACTGTTGCTTTTAAAATTGCTGCAGTTCCATTTCATCAGTGGACACCCGATGTCTACGAAGGATCTCCC
ACGCCGGTAGTAGCTTTTTTGTCAGTTGGTTCAAAGACTGCTGGATTTGCTTTTGCAATAAGAATTTTAACCACCTCTTT
TTCTTCTTTTGATGAACAATGGAAATTACTATTTACCATTTTAGCTATTTTAAGTATGGCCTTAGGAAATATTGTTGCAT
TGGCCCAAACCTCAATGAAAAGAATGTTGGCTTATAGTTCAATTGGTCAAGCAGGATTTGTAATGATTGGAATAGTTTCC
GGGACTCAAGATGGTCTTTCAGCTGCTGTCTTATACTTGGCTGCTTACTTATTTATGAATCTAGGAGCTTTTTCATGTGT
AATACTTTTTTCTCTTAGAACTGGCTCAGATAGAATTTCAGATTATTCAGGCCTGTATCAAAAAGATCCCTTAATTACTT
TGGGTTTGAGTTTATGTCTTCTTTCTCTCGGAGGACTTCCGCCTATGTTAGGTTTTTTCGGGAAAATATATTTGTTCTTT
GCAGGCTGGGCTAATCATCAATATCTTTTAGTAGTTGTTGGATTGGTTACTTCAGTAATTTCAATTTATTACTACATCTC
GGTTATAAAAATGATGGTAGTAAAAGAGCCCCAAGAAGCTTCTGAAATAGTAAAATCCTATCCTGAAATCAAATGGAATA
TAATAGGATTACCTCCGTTAAGAATTGCTTTATATACTTGTATAGCTGTGACAGCTTTAGGAGGAATACTTTCTAACCCT
CTTTTCAAATTAGCTAATTCAGCAGTATCAGAAACTCCTTTTTTACAGGATATCTTAGCTGTAACAAATAATATGCTCTA
A

Upstream 100 bases:

>100_bases
AAACAAACTTGCTCAAGAATTTCAAAAATCATACCCACAAATTATAATCAACTTAAGATTAACTCTTACATCCTTACAAT
CAAACATTCAATTTAATTAA

Downstream 100 bases:

>100_bases
AAAATTATTCATAAATGACTAAAAAAGTTGCGACCTTAGAAAAAGTATCTAAGACTTACGGTAAAGATGATCTTATTGTA
AAAGCCTTAGATAATGTAAA

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2 [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASRWAPVICYISLGSSLVSLAFQWSNPVNNAFLGSFNSDNLA
IAFRSIIALSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLVSVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAIYLYGSSFLYGLSGSTNLSTIGVEIINKPSFITSLSLVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILTTSFSSFDEQWKLLFTILAILSMALGNIVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRISDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVVVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEIKWNIIGLPPLRIALYTCIAVTALGGILSNP
LFKLANSAVSETPFLQDILAVTNNML

Sequences:

>Translated_506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASRWAPVICYISLGSSLVSLAFQWSNPVNNAFLGSFNSDNLA
IAFRSIIALSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLVSVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAIYLYGSSFLYGLSGSTNLSTIGVEIINKPSFITSLSLVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILTTSFSSFDEQWKLLFTILAILSMALGNIVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRISDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVVVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEIKWNIIGLPPLRIALYTCIAVTALGGILSNP
LFKLANSAVSETPFLQDILAVTNNML
>Mature_505_residues
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASRWAPVICYISLGSSLVSLAFQWSNPVNNAFLGSFNSDNLAI
AFRSIIALSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLVSVFISLETLSVASYLLSGYLKRDPRSSE
AALKYLLVGSAAAAIYLYGSSFLYGLSGSTNLSTIGVEIINKPSFITSLSLVFVLSTVAFKIAAVPFHQWTPDVYEGSPT
PVVAFLSVGSKTAGFAFAIRILTTSFSSFDEQWKLLFTILAILSMALGNIVALAQTSMKRMLAYSSIGQAGFVMIGIVSG
TQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRISDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFA
GWANHQYLLVVVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEIKWNIIGLPPLRIALYTCIAVTALGGILSNPL
FKLANSAVSETPFLQDILAVTNNML

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=367, Percent_Identity=23.9782016348774, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI145693160, Length=376, Percent_Identity=33.5106382978723, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788829, Length=342, Percent_Identity=26.6081871345029, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=26.9360269360269, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788614, Length=387, Percent_Identity=25.8397932816537, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788613, Length=360, Percent_Identity=23.8888888888889, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54280; Mature: 54149

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASRWAPVICYISLGSSLVSLAF
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCHHHHHHHH
QWSNPVNNAFLGSFNSDNLAIAFRSIIALSTLISLLISWRYTEQSGSPIGEFAAIVLSAT
CCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLVSVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAIYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEEC
SSFLYGLSGSTNLSTIGVEIINKPSFITSLSLVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCCEEEEHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILTTSFSSFDEQWKLLFTILAILSMALGNIVALAQTSMK
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIS
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCHHH
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVVVGLVTSVI
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEIKWNIIGLPPLRIALYTCIAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCH
LFKLANSAVSETPFLQDILAVTNNML
HHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASRWAPVICYISLGSSLVSLAF
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCHHHHHHHH
QWSNPVNNAFLGSFNSDNLAIAFRSIIALSTLISLLISWRYTEQSGSPIGEFAAIVLSAT
CCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLVSVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAIYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEEC
SSFLYGLSGSTNLSTIGVEIINKPSFITSLSLVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCCEEEEHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILTTSFSSFDEQWKLLFTILAILSMALGNIVALAQTSMK
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIS
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCHHH
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVVVGLVTSVI
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEIKWNIIGLPPLRIALYTCIAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCH
LFKLANSAVSETPFLQDILAVTNNML
HHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA