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The map label for this gene is mrcB [H]

Identifier: 123965724

GI number: 123965724

Start: 436888

End: 438657

Strand: Reverse

Name: mrcB [H]

Synonym: P9515_04891

Alternate gene names: 123965724

Gene position: 438657-436888 (Counterclockwise)

Preceding gene: 123965725

Following gene: 123965718

Centisome position: 25.74

GC content: 26.38

Gene sequence:

>1770_bases
GTGAATGAAAAAAAATACAAATATCTTATTATTACCCCCTCAATATTTATTTTCTTTGGTATATTTTTCCCCGTAATTAA
TTTAATTAGGTTTACTTTAAATTCTAATCCACTTAAAAATAATAATAATTCAAAATCAATAACCTATTCTTATGAAATAT
TATCTTCTGATAATAAAATATTAAGCAAATTAAGTAGAAAATTTGATGTCCTTGATGATACAAATTCGATACCATTTTTC
CTAAAATATTCTTTTATCTCTGGAGAGGACAAAAGATTTTTTAAACATAATGGAATTGATTTAATAGGTTTATCAAGAGC
CTTGATTAATAATATTCAAAGTAGGTCCTTTAAGGAGGGTGGAAGTACTATTACGCAGCAAGTAGCTAGATTAATTTTTT
TAAATAATGATTTAAGTTTTCAACGAAAAATTAAAGAAATGATAATATCCTTAATACTAGATTTTAAATACAGTAAATCC
CAGATTTTAAAATTATATCTAAATAATATTTACCTAGGATCTGGAGCTTACGGAGTAAATGAGGCCTCTCAAGTTTATTT
TGGGAAATTTATTAACGAATTAACATTATCTGAGGTTGCCTTAATAGCAGGTTTAGCTCCTGCTCCTTCAATTTATTCAC
CTTATGAAAATATTGATTTAGCTATTAAACAAAGAAATAAAATTTTAGAATCTATGTATTTAGATGGCCACATATCATTA
GGTGAAAAAGATTCAGCTCTTCAGGAAAAAGTTAAATTAAATTATCAAATAGATAACAAAAATCTTAACGATAAATTATT
AGTAAATTATATTTTGGAGGAAGCAAATAAAATTATTAAGACTCATAAAAAATATAAGTTTTTAAAAATCAAATCAAGTA
TCAACAAAAATTGGCAAGAAATTGGACAACATATTTCAAAGTATATAGGTCCTAATAATATTGAAATAGCTTTGGTATCA
ATTGAATCCAATACAGGTTTAATTAGAACAATGATCACCAGCAAAAATCCATCAATAAACGAATTTAATAGAGTCACTTC
AGCAGTAAGACCTTTAGGCTCAACTTTTAAAATTATTCCTTATGCAGCAGCACTTATAGAAGGTAGAAAGTTAAGTGATA
AATTCGAGGATATTCCTACTTGCTGGAAAGATTATTGTCCAAACAATTTTTCTGAAAACTACCTTGGTAAGATATCCTTA
ACTGAATCTTTTAAAAAATCTTCTAATATAGTTCCATTAAAAATAGCTAATAATATAGGCCTAAAAAAAATTATAAATTT
AGCTAATTTATTTGGACTAGGATATGAACAAAAGCTTGAAGAATTTCTACCATTAGCTATTGGATCTTATGGAGATAACC
TTCTAAATATTACAAACGCCTATGCAGTTTTGAATAGCAATGGAAATCTCTATAAGCCTAGCATTATTGAAAAAATAGAG
TCAAAAAATCAAGAATTAATTTGGGAAAACAAATTTGTTCATAAGAAAATATTAGCTCCTAAAGTAAACAAAAATTTAAA
TAATCTACTTGAGAAATCTGTATCAGAAGGAACTTCGATAGGAGCTTCAATAAAAGGAGAAAGGATATTTGGAAAGACAG
GTACCTCGGATGAGAATAGAGACCTTTGGTTTATTGGTTCAATTAAAAATCTTACTACTGGAGTTTGGATAGGTTTTGAT
GAAAACAAAAAATCTAACTTATCAAGTGGTAATGCAGCTTATTTCTGGAAAAAATTTATAAGTAAAATTTTTGATTTAAA
AATTCAATAA

Upstream 100 bases:

>100_bases
AAATTTGATGTCAAATATCAGGCAAGCGCACAGCCATTCCTAATAACTGGAATGTTAGTAACTGCCTTAGCAATAGGACA
TAGTTTTTTAGTAGCTTAAA

Downstream 100 bases:

>100_bases
TAAAAAGTTTTAGTTTTACTTATAGGATATTGTTGCTGCATAGAATCCATCTCCTGGCTTATCAAATCTTGGTAAAATTT
GTCTTTCACTATCTAATTTA

Product: putative penicillin binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MNEKKYKYLIITPSIFIFFGIFFPVINLIRFTLNSNPLKNNNNSKSITYSYEILSSDNKILSKLSRKFDVLDDTNSIPFF
LKYSFISGEDKRFFKHNGIDLIGLSRALINNIQSRSFKEGGSTITQQVARLIFLNNDLSFQRKIKEMIISLILDFKYSKS
QILKLYLNNIYLGSGAYGVNEASQVYFGKFINELTLSEVALIAGLAPAPSIYSPYENIDLAIKQRNKILESMYLDGHISL
GEKDSALQEKVKLNYQIDNKNLNDKLLVNYILEEANKIIKTHKKYKFLKIKSSINKNWQEIGQHISKYIGPNNIEIALVS
IESNTGLIRTMITSKNPSINEFNRVTSAVRPLGSTFKIIPYAAALIEGRKLSDKFEDIPTCWKDYCPNNFSENYLGKISL
TESFKKSSNIVPLKIANNIGLKKIINLANLFGLGYEQKLEEFLPLAIGSYGDNLLNITNAYAVLNSNGNLYKPSIIEKIE
SKNQELIWENKFVHKKILAPKVNKNLNNLLEKSVSEGTSIGASIKGERIFGKTGTSDENRDLWFIGSIKNLTTGVWIGFD
ENKKSNLSSGNAAYFWKKFISKIFDLKIQ

Sequences:

>Translated_589_residues
MNEKKYKYLIITPSIFIFFGIFFPVINLIRFTLNSNPLKNNNNSKSITYSYEILSSDNKILSKLSRKFDVLDDTNSIPFF
LKYSFISGEDKRFFKHNGIDLIGLSRALINNIQSRSFKEGGSTITQQVARLIFLNNDLSFQRKIKEMIISLILDFKYSKS
QILKLYLNNIYLGSGAYGVNEASQVYFGKFINELTLSEVALIAGLAPAPSIYSPYENIDLAIKQRNKILESMYLDGHISL
GEKDSALQEKVKLNYQIDNKNLNDKLLVNYILEEANKIIKTHKKYKFLKIKSSINKNWQEIGQHISKYIGPNNIEIALVS
IESNTGLIRTMITSKNPSINEFNRVTSAVRPLGSTFKIIPYAAALIEGRKLSDKFEDIPTCWKDYCPNNFSENYLGKISL
TESFKKSSNIVPLKIANNIGLKKIINLANLFGLGYEQKLEEFLPLAIGSYGDNLLNITNAYAVLNSNGNLYKPSIIEKIE
SKNQELIWENKFVHKKILAPKVNKNLNNLLEKSVSEGTSIGASIKGERIFGKTGTSDENRDLWFIGSIKNLTTGVWIGFD
ENKKSNLSSGNAAYFWKKFISKIFDLKIQ
>Mature_589_residues
MNEKKYKYLIITPSIFIFFGIFFPVINLIRFTLNSNPLKNNNNSKSITYSYEILSSDNKILSKLSRKFDVLDDTNSIPFF
LKYSFISGEDKRFFKHNGIDLIGLSRALINNIQSRSFKEGGSTITQQVARLIFLNNDLSFQRKIKEMIISLILDFKYSKS
QILKLYLNNIYLGSGAYGVNEASQVYFGKFINELTLSEVALIAGLAPAPSIYSPYENIDLAIKQRNKILESMYLDGHISL
GEKDSALQEKVKLNYQIDNKNLNDKLLVNYILEEANKIIKTHKKYKFLKIKSSINKNWQEIGQHISKYIGPNNIEIALVS
IESNTGLIRTMITSKNPSINEFNRVTSAVRPLGSTFKIIPYAAALIEGRKLSDKFEDIPTCWKDYCPNNFSENYLGKISL
TESFKKSSNIVPLKIANNIGLKKIINLANLFGLGYEQKLEEFLPLAIGSYGDNLLNITNAYAVLNSNGNLYKPSIIEKIE
SKNQELIWENKFVHKKILAPKVNKNLNNLLEKSVSEGTSIGASIKGERIFGKTGTSDENRDLWFIGSIKNLTTGVWIGFD
ENKKSNLSSGNAAYFWKKFISKIFDLKIQ

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=525, Percent_Identity=26.0952380952381, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082258, Length=412, Percent_Identity=28.6407766990291, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788867, Length=498, Percent_Identity=26.3052208835341, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789601, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 66855; Mature: 66855

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKKYKYLIITPSIFIFFGIFFPVINLIRFTLNSNPLKNNNNSKSITYSYEILSSDNKI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHH
LSKLSRKFDVLDDTNSIPFFLKYSFISGEDKRFFKHNGIDLIGLSRALINNIQSRSFKEG
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
GSTITQQVARLIFLNNDLSFQRKIKEMIISLILDFKYSKSQILKLYLNNIYLGSGAYGVN
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC
EASQVYFGKFINELTLSEVALIAGLAPAPSIYSPYENIDLAIKQRNKILESMYLDGHISL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GEKDSALQEKVKLNYQIDNKNLNDKLLVNYILEEANKIIKTHKKYKFLKIKSSINKNWQE
CCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHCCCHHH
IGQHISKYIGPNNIEIALVSIESNTGLIRTMITSKNPSINEFNRVTSAVRPLGSTFKIIP
HHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEHHH
YAAALIEGRKLSDKFEDIPTCWKDYCPNNFSENYLGKISLTESFKKSSNIVPLKIANNIG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCEEEEEECCCCC
LKKIINLANLFGLGYEQKLEEFLPLAIGSYGDNLLNITNAYAVLNSNGNLYKPSIIEKIE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHEEEEEEEEECCCCCEECCHHHHHHH
SKNQELIWENKFVHKKILAPKVNKNLNNLLEKSVSEGTSIGASIKGERIFGKTGTSDENR
CCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCC
DLWFIGSIKNLTTGVWIGFDENKKSNLSSGNAAYFWKKFISKIFDLKIQ
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNEKKYKYLIITPSIFIFFGIFFPVINLIRFTLNSNPLKNNNNSKSITYSYEILSSDNKI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHH
LSKLSRKFDVLDDTNSIPFFLKYSFISGEDKRFFKHNGIDLIGLSRALINNIQSRSFKEG
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
GSTITQQVARLIFLNNDLSFQRKIKEMIISLILDFKYSKSQILKLYLNNIYLGSGAYGVN
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC
EASQVYFGKFINELTLSEVALIAGLAPAPSIYSPYENIDLAIKQRNKILESMYLDGHISL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GEKDSALQEKVKLNYQIDNKNLNDKLLVNYILEEANKIIKTHKKYKFLKIKSSINKNWQE
CCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHCCCHHH
IGQHISKYIGPNNIEIALVSIESNTGLIRTMITSKNPSINEFNRVTSAVRPLGSTFKIIP
HHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEHHH
YAAALIEGRKLSDKFEDIPTCWKDYCPNNFSENYLGKISLTESFKKSSNIVPLKIANNIG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCEEEEEECCCCC
LKKIINLANLFGLGYEQKLEEFLPLAIGSYGDNLLNITNAYAVLNSNGNLYKPSIIEKIE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHEEEEEEEEECCCCCEECCHHHHHHH
SKNQELIWENKFVHKKILAPKVNKNLNNLLEKSVSEGTSIGASIKGERIFGKTGTSDENR
CCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCC
DLWFIGSIKNLTTGVWIGFDENKKSNLSSGNAAYFWKKFISKIFDLKIQ
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]