Click here to switch to the map view.

The map label for this gene is trmH [H]

Identifier: 123965718

GI number: 123965718

Start: 431672

End: 432346

Strand: Reverse

Name: trmH [H]

Synonym: P9515_04831

Alternate gene names: 123965718

Gene position: 432346-431672 (Counterclockwise)

Preceding gene: 123965724

Following gene: 123965714

Centisome position: 25.37

GC content: 31.11

Gene sequence:

>675_bases
ATGTCGATTTTACCAAGAAGATTTGAGAGGATAAAAAATGTTTTAAATTGTCGAATGGAAAACTTAACTGTTTTAGTAGA
GGCAGTAAATAAGCCCCATAATTTATCTGCAATATTAAGAACCTGTGATGCAGCAGGAGTTTTTGAAGCGAATTTCATTT
GTAAAAGAGATGAAGTTAAAACTTTTAACAGTACAGCTCAAGGTAGTCAAAAATGGGTCAAATTGAATAATCATGAAACA
ACAATTTCTGCAGTATCTCATCTAAAAAAGAAAGGATTTAAATTATATGGAACAACACTTAATGATAGCTCTACTGATTA
CAGGAATTTTGACTATGCAGAAAATACATGTTTTGTTTTAGGAGCAGAGAAGTGGGGATTAAGTGATGAACTTATCTCCA
AGGTTGATGAATCAATCTTTATCCCTATGACAGGAATGGTCCAATCTTTGAATGTTTCAGTAGCTGCCTCTATTTTACTT
TTTGAAGCTATTCGCCAAAGACAAATTAAAAGTTTACTTCCTTTAAATGGGGAAGGTTTAAGTCAAAAAGAATATCAAAA
GACATTATTTGAATGGAGTTATCCTGAGTTAGCCTCTATTTATAAAAAGTCTGGAAATAAATATCCAACCCTTAATCAGG
ATGGAGAAATTAATGAAGTTATAAAAAAACAATAA

Upstream 100 bases:

>100_bases
TGGTAGTAAAAGATAATTAATTATTCAAACTAAAATTTGTATATTTAAAAATTTTTTTTGGGATTAATATAGTTAAGATC
ATAATTAAAACATTTTTCAA

Downstream 100 bases:

>100_bases
TAAAAAAAAGTCAACTATTGCTTCTCAAAAATATTTTCTATTTCTTCTCCTATAAAAGAAAGACCTAGGACTAAAAAAAA
CATTGCTAATCCAGGGAATA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSILPRRFERIKNVLNCRMENLTVLVEAVNKPHNLSAILRTCDAAGVFEANFICKRDEVKTFNSTAQGSQKWVKLNNHET
TISAVSHLKKKGFKLYGTTLNDSSTDYRNFDYAENTCFVLGAEKWGLSDELISKVDESIFIPMTGMVQSLNVSVAASILL
FEAIRQRQIKSLLPLNGEGLSQKEYQKTLFEWSYPELASIYKKSGNKYPTLNQDGEINEVIKKQ

Sequences:

>Translated_224_residues
MSILPRRFERIKNVLNCRMENLTVLVEAVNKPHNLSAILRTCDAAGVFEANFICKRDEVKTFNSTAQGSQKWVKLNNHET
TISAVSHLKKKGFKLYGTTLNDSSTDYRNFDYAENTCFVLGAEKWGLSDELISKVDESIFIPMTGMVQSLNVSVAASILL
FEAIRQRQIKSLLPLNGEGLSQKEYQKTLFEWSYPELASIYKKSGNKYPTLNQDGEINEVIKKQ
>Mature_223_residues
SILPRRFERIKNVLNCRMENLTVLVEAVNKPHNLSAILRTCDAAGVFEANFICKRDEVKTFNSTAQGSQKWVKLNNHETT
ISAVSHLKKKGFKLYGTTLNDSSTDYRNFDYAENTCFVLGAEKWGLSDELISKVDESIFIPMTGMVQSLNVSVAASILLF
EAIRQRQIKSLLPLNGEGLSQKEYQKTLFEWSYPELASIYKKSGNKYPTLNQDGEINEVIKKQ

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=154, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790083, Length=213, Percent_Identity=45.0704225352113, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=36.4864864864865, Blast_Score=82, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355819, Length=149, Percent_Identity=28.8590604026846, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25395; Mature: 25264

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPRRFERIKNVLNCRMENLTVLVEAVNKPHNLSAILRTCDAAGVFEANFICKRDEVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCHHH
TFNSTAQGSQKWVKLNNHETTISAVSHLKKKGFKLYGTTLNDSSTDYRNFDYAENTCFVL
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEE
GAEKWGLSDELISKVDESIFIPMTGMVQSLNVSVAASILLFEAIRQRQIKSLLPLNGEGL
ECCCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQKEYQKTLFEWSYPELASIYKKSGNKYPTLNQDGEINEVIKKQ
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SILPRRFERIKNVLNCRMENLTVLVEAVNKPHNLSAILRTCDAAGVFEANFICKRDEVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCHHH
TFNSTAQGSQKWVKLNNHETTISAVSHLKKKGFKLYGTTLNDSSTDYRNFDYAENTCFVL
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEE
GAEKWGLSDELISKVDESIFIPMTGMVQSLNVSVAASILLFEAIRQRQIKSLLPLNGEGL
ECCCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQKEYQKTLFEWSYPELASIYKKSGNKYPTLNQDGEINEVIKKQ
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]