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The map label for this gene is metB [H]

Identifier: 123965705

GI number: 123965705

Start: 419334

End: 420497

Strand: Reverse

Name: metB [H]

Synonym: P9515_04701

Alternate gene names: 123965705

Gene position: 420497-419334 (Counterclockwise)

Preceding gene: 123965706

Following gene: 123965704

Centisome position: 24.67

GC content: 32.65

Gene sequence:

>1164_bases
ATGGGAAATAAGGAAAATAATATCAAAAAGCCAGGCGTCAAAACCTTAACAATTCACCATGGAGAAACATTTGCCGAAGA
GACAGGTTGTGTTATGCCTCCAATTTTCTCAACATCTACTTTCAAATATGGAAATAAACAAAATTTCGATTACACCAGGT
CAGGTAATCCAAATTTTAAAATTCTAGAAAATATACTTAAATCAGTAGAAGATTCTAAATACTGTACAGTTTTTGGATCC
GGCATAAGTGCAGTAACTGCAATAACATCCACATTAAAATCAGGAGATAAGATACTTTGCGAGTCTAATCTCTATGGTTG
TACGGTAAGAATGTTTGATAAAATTTTCAAAAAATTTGGGATAGAAACTCTATACACAGATTTTACGGATAAAGAAAGTA
TCAAAAAAATTAAGGACTTTAAGCCAACTTTAATATGGCTCGAAAGTCCAACAAATCCACTATTAAAAATACTTGATATT
AAATCGATCTGTGACGAAGCGAATAAACACAAAATACCTGTGGTTCTAGATAATACCTTTACTACAGCTCTCATTCAAAA
ACCTTTAGAACTTGGAGCAACACTATCGCTAATAAGTACAACAAAATTTATAAATGGTCATAGTGATGCTCTTGGTGGAG
CGGTGCTAACTAATGATGAGGAATGGAATAGTAAGCTACTTTTCTCTCAAAAAGCTTTAGGACTTCAACCCTCGCCCTTT
GATTCATGGCTAATTACAAGAGGAGTAAAAACTCTCCCTCTAAGAATCGAGCAACAAACAAAAAGTGCAGAAATAATTTC
TAAGGAATTTGAAAACCATAGGATCATTAGAAAAGCTCTTTATCCCTTTAATCAAAAACATCCACAATTTAATTTAGCAA
AATCACAAATGAAATCTGGTGGTTCTATGATCACTCTTAAATTGAACTTAAATAAAGCAGACACTTTTAATTTTTGTAAA
TCTCTCAAATATTTCTCATTAGCAGAGAGTCTTGGTGGAGTTGAAAGTTTAATTTGCCACCCCGCAACAATGACTCATGC
ATCTGTTGATGATAAAACAAAAAATCTCCTAGGTATTGACGATTCTCTTGTACGATTATCGGTTGGTTGTGAAGATACAA
ATGATTTAATCTCAGATATCTTATTTGCCTTAAATAAATTTTGA

Upstream 100 bases:

>100_bases
TGAATATTATTCAAGAAAAGTTTAAAAAAACTTTTCTTTGGATTATTAAATCTCTCCTCGATAAGAAGAGAGATTTTATT
AAATTCTTATTTTGAAAATA

Downstream 100 bases:

>100_bases
AAATTGAGAGATTTACTTAAAAATCCCATATGGAGAAATTTAGAGTTGGGTTTTTCGATCCCTGATAGTAAACATGCTGT
TTCTGTAGCATTACCAACTT

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGNKENNIKKPGVKTLTIHHGETFAEETGCVMPPIFSTSTFKYGNKQNFDYTRSGNPNFKILENILKSVEDSKYCTVFGS
GISAVTAITSTLKSGDKILCESNLYGCTVRMFDKIFKKFGIETLYTDFTDKESIKKIKDFKPTLIWLESPTNPLLKILDI
KSICDEANKHKIPVVLDNTFTTALIQKPLELGATLSLISTTKFINGHSDALGGAVLTNDEEWNSKLLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEIISKEFENHRIIRKALYPFNQKHPQFNLAKSQMKSGGSMITLKLNLNKADTFNFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDSLVRLSVGCEDTNDLISDILFALNKF

Sequences:

>Translated_387_residues
MGNKENNIKKPGVKTLTIHHGETFAEETGCVMPPIFSTSTFKYGNKQNFDYTRSGNPNFKILENILKSVEDSKYCTVFGS
GISAVTAITSTLKSGDKILCESNLYGCTVRMFDKIFKKFGIETLYTDFTDKESIKKIKDFKPTLIWLESPTNPLLKILDI
KSICDEANKHKIPVVLDNTFTTALIQKPLELGATLSLISTTKFINGHSDALGGAVLTNDEEWNSKLLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEIISKEFENHRIIRKALYPFNQKHPQFNLAKSQMKSGGSMITLKLNLNKADTFNFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDSLVRLSVGCEDTNDLISDILFALNKF
>Mature_386_residues
GNKENNIKKPGVKTLTIHHGETFAEETGCVMPPIFSTSTFKYGNKQNFDYTRSGNPNFKILENILKSVEDSKYCTVFGSG
ISAVTAITSTLKSGDKILCESNLYGCTVRMFDKIFKKFGIETLYTDFTDKESIKKIKDFKPTLIWLESPTNPLLKILDIK
SICDEANKHKIPVVLDNTFTTALIQKPLELGATLSLISTTKFINGHSDALGGAVLTNDEEWNSKLLFSQKALGLQPSPFD
SWLITRGVKTLPLRIEQQTKSAEIISKEFENHRIIRKALYPFNQKHPQFNLAKSQMKSGGSMITLKLNLNKADTFNFCKS
LKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDSLVRLSVGCEDTNDLISDILFALNKF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.8839779005525, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=37.0165745856354, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=37.6731301939058, Blast_Score=221, Evalue=9e-58,
Organism=Escherichia coli, GI1790375, Length=370, Percent_Identity=35.6756756756757, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789383, Length=338, Percent_Identity=26.6272189349112, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560056, Length=368, Percent_Identity=41.0326086956522, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=41.0326086956522, Blast_Score=287, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI71980708, Length=377, Percent_Identity=31.8302387267904, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=37.8238341968912, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321254, Length=398, Percent_Identity=30.4020100502513, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6321904, Length=389, Percent_Identity=29.305912596401, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=338, Percent_Identity=28.4023668639053, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323333, Length=404, Percent_Identity=28.960396039604, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=39.5833333333333, Blast_Score=285, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 43131; Mature: 43000

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKENNIKKPGVKTLTIHHGETFAEETGCVMPPIFSTSTFKYGNKQNFDYTRSGNPNFK
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKSVEDSKYCTVFGSGISAVTAITSTLKSGDKILCESNLYGCTVRMFDKIFKKFG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHC
IETLYTDFTDKESIKKIKDFKPTLIWLESPTNPLLKILDIKSICDEANKHKIPVVLDNTF
CHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCCH
TTALIQKPLELGATLSLISTTKFINGHSDALGGAVLTNDEEWNSKLLFSQKALGLQPSPF
HHHHHHHHHHHCCEEEHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEIISKEFENHRIIRKALYPFNQKHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMITLKLNLNKADTFNFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHEECCC
DSLVRLSVGCEDTNDLISDILFALNKF
CCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNKENNIKKPGVKTLTIHHGETFAEETGCVMPPIFSTSTFKYGNKQNFDYTRSGNPNFK
CCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKSVEDSKYCTVFGSGISAVTAITSTLKSGDKILCESNLYGCTVRMFDKIFKKFG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHC
IETLYTDFTDKESIKKIKDFKPTLIWLESPTNPLLKILDIKSICDEANKHKIPVVLDNTF
CHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCCH
TTALIQKPLELGATLSLISTTKFINGHSDALGGAVLTNDEEWNSKLLFSQKALGLQPSPF
HHHHHHHHHHHCCEEEHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEIISKEFENHRIIRKALYPFNQKHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMITLKLNLNKADTFNFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHEECCC
DSLVRLSVGCEDTNDLISDILFALNKF
CCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]