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The map label for this gene is mdeA [H]

Identifier: 123965704

GI number: 123965704

Start: 417861

End: 419288

Strand: Reverse

Name: mdeA [H]

Synonym: P9515_04691

Alternate gene names: 123965704

Gene position: 419288-417861 (Counterclockwise)

Preceding gene: 123965705

Following gene: 123965703

Centisome position: 24.6

GC content: 30.67

Gene sequence:

>1428_bases
TTGGGTTTTTCGATCCCTGATAGTAAACATGCTGTTTCTGTAGCATTACCAACTTGGAATGATGTAATTAACTATGAGGA
AAAAGATCCAAAATGTATAGAAGCATTAAAGTCAATCTATCCTCGTTTTGGACTCAATCCTTTAGTAAAAAGATTATGTG
AAAAGATAAAAAAAGAAAGTCAATTCAATAATAAGAATATTTGGCCATATCCAGATGAAAGAATAGCTTTAAAAGCAAAA
AAATACTGTGATAAAAATACTTTTCAAGGATATTCATATATAGAAAGAAGGCACAATTTTACTTTATTAATCACTCAAGA
ATCAGCAAATATATATGCAAGATCTTTTTGGCAACATTCGGGTCTTGGTCTATCTTCAAGAGCGGCAGCAATTGAATTAG
GCCTTGAGCATTGCCCATCAAAATCTTTAGCCAATGAAAGTTATCAAAGAATAAAAAATAGAATTTCCAAGTTTACAAAA
ACGGACTCTAATGATATTCACCTCACTTCATCAGGCATGTCTGCGTTATACACAATATTAGAAATTATATATAAATTATT
TCCCGAAAAACCTACTCTACAAATTGGTTTCCCATATGTTGATGTACTTAAATTACCAATGAATATCTTTCATGGAGCCA
AGCTAATAACAGAAGAAGATTGCAAGGATATTGAATTAGAAATTTTAAGAATAAATCCAGCAGCCTTGATTATTGAATTA
CCAAGTAATCCAATGCTCAAATGTGTAAACATAAAAAAAATTTCAAAAATAGCAAATAAATTAAATATACCTGTGATAGT
TGACGATACCATTGGTTCAAATTTGAATATAAATTCCCTAGAACATGCAGATATCGTTTTCACCTCACTTACAAAAATCT
TTTCAGGTAGCGGAGATATTCTGGCTGGCTCATTAATACTAAATCCAAAAAGCAGATGGATTGATCAGTTTAGAAATGCA
TTAAAGCAAATTAACCTTCCAATACTTTCAGATGGAGATATAGTTTCTTTAGAAAAAGTTAGTAGAGATGTAAAACAACG
AGTTTTTCAGCAAAATAAAGCTTGTTTGGAATTAAAAAAAAGATTAGAGACCCATAAAGAAGTAAAAAATATTTTCCATC
CAGAAAATTGTCCCAATTTCAATTCCATACTAACTTCTGATGGGGGATATGGTTGCTTATTATCATTTGAATTAAAAGGA
GGAAAAGAAAAAGCTAAAAAGTTTTATGATTCTCTTCAAATATCTAAAGGACCTAGTTTAGGTACGAAATTTACACTTGT
ATGTCCTTATGTTTTATTAGCTCATTATGAAGAATTGGAGTGGGCTGAGAGTTTTGGTGTACCTTCACACCTTTTAAGGG
TATCTGTAGGACTAGAAGACGAAAATCACTTATGGCATACCTTTTCTGAAGCATTAAATAATTTTTAA

Upstream 100 bases:

>100_bases
TGAAGATACAAATGATTTAATCTCAGATATCTTATTTGCCTTAAATAAATTTTGAAAATTGAGAGATTTACTTAAAAATC
CCATATGGAGAAATTTAGAG

Downstream 100 bases:

>100_bases
ATCCCTAGTATTTACCGTATAGAAACTTATATACTCTTTTTACGAAAAATAGTTAATATTAAAATATATTTTCTTAAAAC
ATAAATGGCAAAAATTTATG

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MGFSIPDSKHAVSVALPTWNDVINYEEKDPKCIEALKSIYPRFGLNPLVKRLCEKIKKESQFNNKNIWPYPDERIALKAK
KYCDKNTFQGYSYIERRHNFTLLITQESANIYARSFWQHSGLGLSSRAAAIELGLEHCPSKSLANESYQRIKNRISKFTK
TDSNDIHLTSSGMSALYTILEIIYKLFPEKPTLQIGFPYVDVLKLPMNIFHGAKLITEEDCKDIELEILRINPAALIIEL
PSNPMLKCVNIKKISKIANKLNIPVIVDDTIGSNLNINSLEHADIVFTSLTKIFSGSGDILAGSLILNPKSRWIDQFRNA
LKQINLPILSDGDIVSLEKVSRDVKQRVFQQNKACLELKKRLETHKEVKNIFHPENCPNFNSILTSDGGYGCLLSFELKG
GKEKAKKFYDSLQISKGPSLGTKFTLVCPYVLLAHYEELEWAESFGVPSHLLRVSVGLEDENHLWHTFSEALNNF

Sequences:

>Translated_475_residues
MGFSIPDSKHAVSVALPTWNDVINYEEKDPKCIEALKSIYPRFGLNPLVKRLCEKIKKESQFNNKNIWPYPDERIALKAK
KYCDKNTFQGYSYIERRHNFTLLITQESANIYARSFWQHSGLGLSSRAAAIELGLEHCPSKSLANESYQRIKNRISKFTK
TDSNDIHLTSSGMSALYTILEIIYKLFPEKPTLQIGFPYVDVLKLPMNIFHGAKLITEEDCKDIELEILRINPAALIIEL
PSNPMLKCVNIKKISKIANKLNIPVIVDDTIGSNLNINSLEHADIVFTSLTKIFSGSGDILAGSLILNPKSRWIDQFRNA
LKQINLPILSDGDIVSLEKVSRDVKQRVFQQNKACLELKKRLETHKEVKNIFHPENCPNFNSILTSDGGYGCLLSFELKG
GKEKAKKFYDSLQISKGPSLGTKFTLVCPYVLLAHYEELEWAESFGVPSHLLRVSVGLEDENHLWHTFSEALNNF
>Mature_474_residues
GFSIPDSKHAVSVALPTWNDVINYEEKDPKCIEALKSIYPRFGLNPLVKRLCEKIKKESQFNNKNIWPYPDERIALKAKK
YCDKNTFQGYSYIERRHNFTLLITQESANIYARSFWQHSGLGLSSRAAAIELGLEHCPSKSLANESYQRIKNRISKFTKT
DSNDIHLTSSGMSALYTILEIIYKLFPEKPTLQIGFPYVDVLKLPMNIFHGAKLITEEDCKDIELEILRINPAALIIELP
SNPMLKCVNIKKISKIANKLNIPVIVDDTIGSNLNINSLEHADIVFTSLTKIFSGSGDILAGSLILNPKSRWIDQFRNAL
KQINLPILSDGDIVSLEKVSRDVKQRVFQQNKACLELKKRLETHKEVKNIFHPENCPNFNSILTSDGGYGCLLSFELKGG
KEKAKKFYDSLQISKGPSLGTKFTLVCPYVLLAHYEELEWAESFGVPSHLLRVSVGLEDENHLWHTFSEALNNF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=323, Percent_Identity=25.3869969040248, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538055, Length=250, Percent_Identity=26.4, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17560056, Length=250, Percent_Identity=26.8, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322970, Length=554, Percent_Identity=31.4079422382672, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6322590, Length=373, Percent_Identity=33.7801608579088, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323557, Length=345, Percent_Identity=34.7826086956522, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=276, Percent_Identity=30.0724637681159, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922554, Length=244, Percent_Identity=27.8688524590164, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 53880; Mature: 53748

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFSIPDSKHAVSVALPTWNDVINYEEKDPKCIEALKSIYPRFGLNPLVKRLCEKIKKES
CCCCCCCCCCEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QFNNKNIWPYPDERIALKAKKYCDKNTFQGYSYIERRHNFTLLITQESANIYARSFWQHS
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHC
GLGLSSRAAAIELGLEHCPSKSLANESYQRIKNRISKFTKTDSNDIHLTSSGMSALYTIL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
EIIYKLFPEKPTLQIGFPYVDVLKLPMNIFHGAKLITEEDCKDIELEILRINPAALIIEL
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCEEEEEEC
PSNPMLKCVNIKKISKIANKLNIPVIVDDTIGSNLNINSLEHADIVFTSLTKIFSGSGDI
CCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
LAGSLILNPKSRWIDQFRNALKQINLPILSDGDIVSLEKVSRDVKQRVFQQNKACLELKK
EEEEEEECCHHHHHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RLETHKEVKNIFHPENCPNFNSILTSDGGYGCLLSFELKGGKEKAKKFYDSLQISKGPSL
HHHHHHHHHHHCCCCCCCCHHHEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
GTKFTLVCPYVLLAHYEELEWAESFGVPSHLLRVSVGLEDENHLWHTFSEALNNF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GFSIPDSKHAVSVALPTWNDVINYEEKDPKCIEALKSIYPRFGLNPLVKRLCEKIKKES
CCCCCCCCCEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QFNNKNIWPYPDERIALKAKKYCDKNTFQGYSYIERRHNFTLLITQESANIYARSFWQHS
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHC
GLGLSSRAAAIELGLEHCPSKSLANESYQRIKNRISKFTKTDSNDIHLTSSGMSALYTIL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
EIIYKLFPEKPTLQIGFPYVDVLKLPMNIFHGAKLITEEDCKDIELEILRINPAALIIEL
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCEEEEEEC
PSNPMLKCVNIKKISKIANKLNIPVIVDDTIGSNLNINSLEHADIVFTSLTKIFSGSGDI
CCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
LAGSLILNPKSRWIDQFRNALKQINLPILSDGDIVSLEKVSRDVKQRVFQQNKACLELKK
EEEEEEECCHHHHHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RLETHKEVKNIFHPENCPNFNSILTSDGGYGCLLSFELKGGKEKAKKFYDSLQISKGPSL
HHHHHHHHHHHCCCCCCCCHHHEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
GTKFTLVCPYVLLAHYEELEWAESFGVPSHLLRVSVGLEDENHLWHTFSEALNNF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]