Click here to switch to the map view.

The map label for this gene is metK

Identifier: 123965579

GI number: 123965579

Start: 317165

End: 318406

Strand: Reverse

Name: metK

Synonym: P9515_03441

Alternate gene names: 123965579

Gene position: 318406-317165 (Counterclockwise)

Preceding gene: 123965580

Following gene: 123965577

Centisome position: 18.68

GC content: 38.41

Gene sequence:

>1242_bases
ATGAGCGATTTTATCTTTACTTCTGAATCCGTTACTGAGGGTCATCCTGACAAGATATGTGATCAAATAAGTGACGCAGT
TTTAGATGCTTTATTGAAAGAGGATCCAGAAAGTAGAGTGGCCTGCGAAACAGTAGTAAATACTGGTCTTTGTTTACTTA
CAGGTGAAATCACCTCAAGAGCAAAGCTCGATTATATAAAACTTGTAAGGAAAGTAATTAAAGAAATTGGTTATGAAGGT
GCAAAAGCGGGAGGCTTTGACTCAAATAGCTGTGCAGTTTTAGTAGCTCTTGATGAGCAATCACCTGATATCTCCCAAGG
AGTAAATGAAGCAGATGATCTGAATGAGGATTTGGAAAATAATACTGGTGCCGGGGACCAAGGGATAATGTTTGGCTATG
CATGCGATGAAACCCCTGAATTAATGCCATTACCAATTAGCTTGGCTCACAGATTAGCAAGACAGCTTGCAAAAGTAAGG
CATGAGAACGTGCTTGAATATCTCCTCCCTGATGGAAAGACCCAAGTAAGTATTGATTACAAAAAAGGGGTTCCTGTCTC
AATAAACACCATTTTAATTTCAACTCAGCACAGAGCCGAGATTGATGGCATCATAAATGAGAAAGAAATTCGTCAAAAGA
TAACAGATGATTTATGGATCAATGTTGTTTTACCGGTAACTGAAGATTTAGAAATCAAACCAAGCAAGGAAAAGACAAGA
TTCTTGGTAAACCCCACAGGTAAATTTGTTGTCGGAGGACCTCAAGGAGATGCGGGACTCACTGGGAGAAAAATAATTGT
GGATACTTATGGTGGCTATGCGAGGCATGGAGGTGGCGCATTCTCAGGTAAAGATCCTACAAAAGTTGACAGATCAGCCG
CTTATGCAGCACGTTATGTAGCTAAAAGCATTGTAAAAGCAAAATTAGCAAAAAAGGCGGAAGTACAATTAAGTTACGCT
ATTGGAGTTGCAAAACCAATTTCAATTCTTGTTGACACTTTTGGAACTAGTGTTATTTCTCAAGATAATTTGAAAGAGCT
AATAGAAAACAATTTTGATTTAAGGCCCGCAGCAATAATAAAAGAATTTGATTTAAGAAATCTACCCAAAAAATTGGGTG
GGGAATTTTACAGAAAGACTGCATCCTATGGACATTTTGGTAGAAATGATCTAAACCTTCCTTGGGAAAGTGTAGAAGTA
AAAGCAGCCCAGCTAGTAGAGGCCTCAAAAGATTTCTTGTAA

Upstream 100 bases:

>100_bases
TTAACAACAAATACAAATGTTTAATGTAATTTCAACTTAATAACTATTGATTAATATTTGCAATTTAAATTAGGATTAAA
AGTAATTATTAAAATTATAA

Downstream 100 bases:

>100_bases
AAATTTATGCGAGATGATTTTTTTGGGGTTTAAATTTTAGGAATAGTGGTGTAACAATATCCATAATAAATTTAAAAAGT
CATTAAAAATACCGATTCTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLKEDPESRVACETVVNTGLCLLTGEITSRAKLDYIKLVRKVIKEIGYEG
AKAGGFDSNSCAVLVALDEQSPDISQGVNEADDLNEDLENNTGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAKVR
HENVLEYLLPDGKTQVSIDYKKGVPVSINTILISTQHRAEIDGIINEKEIRQKITDDLWINVVLPVTEDLEIKPSKEKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVDTFGTSVISQDNLKELIENNFDLRPAAIIKEFDLRNLPKKLGGEFYRKTASYGHFGRNDLNLPWESVEV
KAAQLVEASKDFL

Sequences:

>Translated_413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLKEDPESRVACETVVNTGLCLLTGEITSRAKLDYIKLVRKVIKEIGYEG
AKAGGFDSNSCAVLVALDEQSPDISQGVNEADDLNEDLENNTGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAKVR
HENVLEYLLPDGKTQVSIDYKKGVPVSINTILISTQHRAEIDGIINEKEIRQKITDDLWINVVLPVTEDLEIKPSKEKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVDTFGTSVISQDNLKELIENNFDLRPAAIIKEFDLRNLPKKLGGEFYRKTASYGHFGRNDLNLPWESVEV
KAAQLVEASKDFL
>Mature_412_residues
SDFIFTSESVTEGHPDKICDQISDAVLDALLKEDPESRVACETVVNTGLCLLTGEITSRAKLDYIKLVRKVIKEIGYEGA
KAGGFDSNSCAVLVALDEQSPDISQGVNEADDLNEDLENNTGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAKVRH
ENVLEYLLPDGKTQVSIDYKKGVPVSINTILISTQHRAEIDGIINEKEIRQKITDDLWINVVLPVTEDLEIKPSKEKTRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYAI
GVAKPISILVDTFGTSVISQDNLKELIENNFDLRPAAIIKEFDLRNLPKKLGGEFYRKTASYGHFGRNDLNLPWESVEVK
AAQLVEASKDFL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=54.6599496221663, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=55.1637279596977, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1789311, Length=409, Percent_Identity=53.5452322738386, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=397, Percent_Identity=54.911838790932, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=54.1353383458647, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=397, Percent_Identity=54.1561712846348, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=54.75, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565905, Length=397, Percent_Identity=48.1108312342569, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=52.1367521367521, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=354, Percent_Identity=51.9774011299435, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=337, Percent_Identity=48.9614243323442, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=66.6666666666667, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=397, Percent_Identity=54.1561712846348, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=53.4005037783375, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=397, Percent_Identity=52.896725440806, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=397, Percent_Identity=52.1410579345088, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=397, Percent_Identity=52.1410579345088, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=384, Percent_Identity=51.0416666666667, Blast_Score=341, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=56.4516129032258, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PROM5 (A2BUU2)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010660.1
- ProteinModelPortal:   A2BUU2
- SMR:   A2BUU2
- STRING:   A2BUU2
- GeneID:   4718703
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_03441
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   PMAR167542:P9515ORF_0360-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 45142; Mature: 45011

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIFTSESVTEGHPDKICDQISDAVLDALLKEDPESRVACETVVNTGLCLLTGEITSR
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCC
AKLDYIKLVRKVIKEIGYEGAKAGGFDSNSCAVLVALDEQSPDISQGVNEADDLNEDLEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAKVRHENVLEYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KKGVPVSINTILISTQHRAEIDGIINEKEIRQKITDDLWINVVLPVTEDLEIKPSKEKTR
CCCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVDTFGTSVISQDNLKELIENNFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCCCHHHHH
KEFDLRNLPKKLGGEFYRKTASYGHFGRNDLNLPWESVEVKAAQLVEASKDFL
HHHHHHHCHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFIFTSESVTEGHPDKICDQISDAVLDALLKEDPESRVACETVVNTGLCLLTGEITSR
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCC
AKLDYIKLVRKVIKEIGYEGAKAGGFDSNSCAVLVALDEQSPDISQGVNEADDLNEDLEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAKVRHENVLEYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KKGVPVSINTILISTQHRAEIDGIINEKEIRQKITDDLWINVVLPVTEDLEIKPSKEKTR
CCCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVDTFGTSVISQDNLKELIENNFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCCCHHHHH
KEFDLRNLPKKLGGEFYRKTASYGHFGRNDLNLPWESVEVKAAQLVEASKDFL
HHHHHHHCHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA