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The map label for this gene is ndhK

Identifier: 123965561

GI number: 123965561

Start: 302378

End: 303112

Strand: Reverse

Name: ndhK

Synonym: P9515_03261

Alternate gene names: 123965561

Gene position: 303112-302378 (Counterclockwise)

Preceding gene: 123965562

Following gene: 123965560

Centisome position: 17.79

GC content: 36.73

Gene sequence:

>735_bases
ATGAACAATTCACTATCACCAAAAGCAATTAGAGAGCTTAGAGAAGAAACCTGTAATCCTCTAGGTGCTCCACAAGTAAC
GACTGACTTGAGTGAAAATATCATAATGACTAGTTTAGATGATCTTCATAACTGGGCCAGATTAAGTAGTCTATGGCCAC
TTTTATATGGAACCGCATGCTGTTTCATCGAATTTGCAGCACTGATAGGGTCTAGATTCGATTTTGACAGGTTTGGTTTG
GTACCTAGAAGTTCCCCAAGACAAGCTGATTTACTTATTGTTGCAGGGACCGTAACAATGAAGATGGCGCCAGCATTAGT
GAGATTGTATGAGCAAATGCCAGAGCCAAAGTATGTAATTGCTATGGGAGCTTGCACAATAACTGGTGGGATGTTTAGTG
CAGACTCGACAACTGCAGTAAGAGGGGTTGATAAATTAATCCCGGTTGATTTATATCTTCCCGGATGCCCTCCTAGACCA
GAAGCTATTTTTGACGCGGTTATTAAATTAAGAAAAAAAGTTGGGAATGAAAGTATTCTTGAAAGGAATAAAGCTGAACA
AACTCACAGGTATTTGACTGTCAAACATGATATGAAACTTGTTTTTTCAGAAAATACCGGAGAATATTTAAACAAAAAAT
CTGAAAAAGCAATTAAGTCTTCAGATCTAGAGATGATTGAAGAAAATATAAAAGTTAAAATCGAAGAGAACTTAATTGAT
GAAGAAACAAAATAA

Upstream 100 bases:

>100_bases
GGCTTATTAGCCTTTATTGAAGCTCTCATATTCATCGCAATATTGGTTATTGCACTTGCGTATGCATGGAGAAAAGGAGC
TTTAGAATGGAGTTAAAAAA

Downstream 100 bases:

>100_bases
TGGACCAAAATAATTTATCTAAATCTTCAGAAGAAATAGTTGAACAAAGAGGAATAGTAAGTAATCGACTTTATAAAGAT
GGAATTACAAACAACTCATT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNNSLSPKAIRELREETCNPLGAPQVTTDLSENIIMTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERNKAEQTHRYLTVKHDMKLVFSENTGEYLNKKSEKAIKSSDLEMIEENIKVKIEENLID
EETK

Sequences:

>Translated_244_residues
MNNSLSPKAIRELREETCNPLGAPQVTTDLSENIIMTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERNKAEQTHRYLTVKHDMKLVFSENTGEYLNKKSEKAIKSSDLEMIEENIKVKIEENLID
EETK
>Mature_244_residues
MNNSLSPKAIRELREETCNPLGAPQVTTDLSENIIMTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERNKAEQTHRYLTVKHDMKLVFSENTGEYLNKKSEKAIKSSDLEMIEENIKVKIEENLID
EETK

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=51.7006802721088, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=48.780487804878, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=173, Percent_Identity=46.242774566474, Blast_Score=166, Evalue=7e-42,
Organism=Drosophila melanogaster, GI18859983, Length=171, Percent_Identity=47.3684210526316, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24642371, Length=171, Percent_Identity=47.3684210526316, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651058, Length=143, Percent_Identity=53.1468531468531, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_PROM5 (A2BUS4)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010642.1
- ProteinModelPortal:   A2BUS4
- SMR:   A2BUS4
- STRING:   A2BUS4
- GeneID:   4718684
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_03261
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- BioCyc:   PMAR167542:P9515ORF_0339-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 27327; Mature: 27327

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSLSPKAIRELREETCNPLGAPQVTTDLSENIIMTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERNKAEQTHRYLTVKHDMKLVFSENTGEYLNKKSEKAIKSSDLEMIEENIKVKIEENLID
HHHHHHHHHHEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
EETK
CCCC
>Mature Secondary Structure
MNNSLSPKAIRELREETCNPLGAPQVTTDLSENIIMTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERNKAEQTHRYLTVKHDMKLVFSENTGEYLNKKSEKAIKSSDLEMIEENIKVKIEENLID
HHHHHHHHHHEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
EETK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA