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The map label for this gene is ndhJ

Identifier: 123965560

GI number: 123965560

Start: 301848

End: 302378

Strand: Reverse

Name: ndhJ

Synonym: P9515_03251

Alternate gene names: 123965560

Gene position: 302378-301848 (Counterclockwise)

Preceding gene: 123965561

Following gene: 123965558

Centisome position: 17.74

GC content: 32.96

Gene sequence:

>531_bases
ATGGACCAAAATAATTTATCTAAATCTTCAGAAGAAATAGTTGAACAAAGAGGAATAGTAAGTAATCGACTTTATAAAGA
TGGAATTACAAACAACTCATTAGGAAACGATCACATAGGTGTTGAGCTCCTCTCAGTTAAACCCGAAAAATTATACGAAG
CCATATCTTCTTTAAAAAAATATGGCTTTAATTACCTACAGTGTCAAGGAGGTTATGATGAGGGACCCGGGAAACGTCTT
GTAAGTTTTTACCATTTAATCTCACTTCAAGATATTGAAGAATTAGAAGAGATTAAAGAAATTAGAGTTAAAGTTTTTTT
AAATCGAGATTCAGATTTATCTGTACCTAGCCTTTATAAAATTTTCAAAGGGAGTGATTGGCAGGAAAGAGAAACTTATG
ACATGTACGGGATCAACTTTCAAGATCATCCAAACCCCAAAAGAATTTTGATGCCTGAGGATTGGAGAGGATGGCCTTTA
AGAAAAGATTATATTCAACCTGACTTTTATGAATTACAAGATGCCTATTAG

Upstream 100 bases:

>100_bases
AAAAATCTGAAAAAGCAATTAAGTCTTCAGATCTAGAGATGATTGAAGAAAATATAAAAGTTAAAATCGAAGAGAACTTA
ATTGATGAAGAAACAAAATA

Downstream 100 bases:

>100_bases
ATTTATTAATTTAATTTCTTTAATTTTTTATTAATAAACATGACTGCCCTCGCAAGTCTTCGAGGGTCATGCCTAAGTGT
TGGGGTAATTTTTCTTGAAT

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDQNNLSKSSEEIVEQRGIVSNRLYKDGITNNSLGNDHIGVELLSVKPEKLYEAISSLKKYGFNYLQCQGGYDEGPGKRL
VSFYHLISLQDIEELEEIKEIRVKVFLNRDSDLSVPSLYKIFKGSDWQERETYDMYGINFQDHPNPKRILMPEDWRGWPL
RKDYIQPDFYELQDAY

Sequences:

>Translated_176_residues
MDQNNLSKSSEEIVEQRGIVSNRLYKDGITNNSLGNDHIGVELLSVKPEKLYEAISSLKKYGFNYLQCQGGYDEGPGKRL
VSFYHLISLQDIEELEEIKEIRVKVFLNRDSDLSVPSLYKIFKGSDWQERETYDMYGINFQDHPNPKRILMPEDWRGWPL
RKDYIQPDFYELQDAY
>Mature_176_residues
MDQNNLSKSSEEIVEQRGIVSNRLYKDGITNNSLGNDHIGVELLSVKPEKLYEAISSLKKYGFNYLQCQGGYDEGPGKRL
VSFYHLISLQDIEELEEIKEIRVKVFLNRDSDLSVPSLYKIFKGSDWQERETYDMYGINFQDHPNPKRILMPEDWRGWPL
RKDYIQPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=157, Percent_Identity=37.5796178343949, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71990788, Length=128, Percent_Identity=36.71875, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32563621, Length=128, Percent_Identity=36.71875, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_PROM5 (A2BUS3)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010641.1
- ProteinModelPortal:   A2BUS3
- SMR:   A2BUS3
- STRING:   A2BUS3
- GeneID:   4718683
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_03251
- eggNOG:   COG0852
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- BioCyc:   PMAR167542:P9515ORF_0338-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- InterPro:   IPR020396
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 20695; Mature: 20695

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQNNLSKSSEEIVEQRGIVSNRLYKDGITNNSLGNDHIGVELLSVKPEKLYEAISSLKK
CCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
YGFNYLQCQGGYDEGPGKRLVSFYHLISLQDIEELEEIKEIRVKVFLNRDSDLSVPSLYK
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHH
IFKGSDWQERETYDMYGINFQDHPNPKRILMPEDWRGWPLRKDYIQPDFYELQDAY
HHCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MDQNNLSKSSEEIVEQRGIVSNRLYKDGITNNSLGNDHIGVELLSVKPEKLYEAISSLKK
CCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
YGFNYLQCQGGYDEGPGKRLVSFYHLISLQDIEELEEIKEIRVKVFLNRDSDLSVPSLYK
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHH
IFKGSDWQERETYDMYGINFQDHPNPKRILMPEDWRGWPLRKDYIQPDFYELQDAY
HHCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA