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The map label for this gene is ppnK [H]

Identifier: 123965420

GI number: 123965420

Start: 169953

End: 170852

Strand: Reverse

Name: ppnK [H]

Synonym: P9515_01851

Alternate gene names: 123965420

Gene position: 170852-169953 (Counterclockwise)

Preceding gene: 123965421

Following gene: 123965419

Centisome position: 10.03

GC content: 29.44

Gene sequence:

>900_bases
GTGCTTATCATTTATAGATCTAACAGCTCAGTTGCTAAAGAAGCATCTATATTTTGTGAGAAGGCTCTCAAAGATAGAAA
TATTAAATCAAAGAGTATAGGAAGTGATTTTGATACAAAAAAAATAGAGCAATATCTTTTTAATCTTCCTTCATTACCTG
ACTTATTAATTGTTCTTGGAGGTGATGGGACTGTTCTTAAATCTGCTAATGCATTAGTTCATTATGATATCCCATTACTA
AGTTTTAATATTGGAGGCAATTTAGGATTCTTAACTCAAGAAAAAGATTTTTTATTTGACAAATCATTTATCAAAATTTT
GGAAAAGGAAGAATTCACAATTGATTTAAGAAACAGGTTGCATTGTGAAGTTTATAGTAAAGAAAATCTTGGAGTACGAA
AAATAATCAAGAGCTATGATGCATTAAATGATTTTTATTTTAAATCCGTTGAAGAAGATATTTCTCCAACAAATCAAATT
CAAATAGAAATAGATAATGAAAAAGTAAACGAATATAAAGGTGATGGTTTAATTATTTCTTCCTCAACTGGTTCTACAGC
ATACTCTATGGCCGCAGGGGGGCCAATAGTTCACCCTTCAATAAACGCATTTGTAATCAATCCAATATGTCCTATGAGCT
TAGCCAGCCGTCCAATTATTATTCCTGATACAAGTAAAGTAATCATTAGAGCAGTTCAAAAGAATAAAAGAGAAATAAAA
CTATGGAAAGATGGATCAAAGTGCATGACTGTAAAAGAAAATGATTATTGCGAAATAAATAAAGGAACAAAGCCCTGTAA
AATGATTAAGTTCAATAAAAGCATTTCTTATTACATTACTTTGATCAAAAAGCTTGATTGGAAAGGGAATTTATCTTTAA
AAAATAATCAACTCAATTAG

Upstream 100 bases:

>100_bases
TTAGCTATATTGTTATCTCTTTATAGAAATCGAGTTACTGTCGATATGGAAAGTTTTAATTTATTAAAGTGGTAAAAAGC
ATCAAGTGAAACTTGCATTG

Downstream 100 bases:

>100_bases
ATGACATTAGAGATTGAAAGGCGATTTCTTATCAAAAATGATCAATGGAAAGCATTTATTACTCATAAAACTTTTATAGA
GCAGGGCTACCTTTCATATG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLIIYRSNSSVAKEASIFCEKALKDRNIKSKSIGSDFDTKKIEQYLFNLPSLPDLLIVLGGDGTVLKSANALVHYDIPLL
SFNIGGNLGFLTQEKDFLFDKSFIKILEKEEFTIDLRNRLHCEVYSKENLGVRKIIKSYDALNDFYFKSVEEDISPTNQI
QIEIDNEKVNEYKGDGLIISSSTGSTAYSMAAGGPIVHPSINAFVINPICPMSLASRPIIIPDTSKVIIRAVQKNKREIK
LWKDGSKCMTVKENDYCEINKGTKPCKMIKFNKSISYYITLIKKLDWKGNLSLKNNQLN

Sequences:

>Translated_299_residues
MLIIYRSNSSVAKEASIFCEKALKDRNIKSKSIGSDFDTKKIEQYLFNLPSLPDLLIVLGGDGTVLKSANALVHYDIPLL
SFNIGGNLGFLTQEKDFLFDKSFIKILEKEEFTIDLRNRLHCEVYSKENLGVRKIIKSYDALNDFYFKSVEEDISPTNQI
QIEIDNEKVNEYKGDGLIISSSTGSTAYSMAAGGPIVHPSINAFVINPICPMSLASRPIIIPDTSKVIIRAVQKNKREIK
LWKDGSKCMTVKENDYCEINKGTKPCKMIKFNKSISYYITLIKKLDWKGNLSLKNNQLN
>Mature_299_residues
MLIIYRSNSSVAKEASIFCEKALKDRNIKSKSIGSDFDTKKIEQYLFNLPSLPDLLIVLGGDGTVLKSANALVHYDIPLL
SFNIGGNLGFLTQEKDFLFDKSFIKILEKEEFTIDLRNRLHCEVYSKENLGVRKIIKSYDALNDFYFKSVEEDISPTNQI
QIEIDNEKVNEYKGDGLIISSSTGSTAYSMAAGGPIVHPSINAFVINPICPMSLASRPIIIPDTSKVIIRAVQKNKREIK
LWKDGSKCMTVKENDYCEINKGTKPCKMIKFNKSISYYITLIKKLDWKGNLSLKNNQLN

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788968, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320794, Length=250, Percent_Identity=32.4, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322509, Length=246, Percent_Identity=30.4878048780488, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6325068, Length=179, Percent_Identity=35.195530726257, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28573828, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28573830, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28573832, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI161077047, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28573826, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129957, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281363321, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281363323, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653424, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653422, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 33740; Mature: 33740

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIYRSNSSVAKEASIFCEKALKDRNIKSKSIGSDFDTKKIEQYLFNLPSLPDLLIVLG
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEC
GDGTVLKSANALVHYDIPLLSFNIGGNLGFLTQEKDFLFDKSFIKILEKEEFTIDLRNRL
CCCCEEECCCEEEEECCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCE
HCEVYSKENLGVRKIIKSYDALNDFYFKSVEEDISPTNQIQIEIDNEKVNEYKGDGLIIS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEE
SSTGSTAYSMAAGGPIVHPSINAFVINPICPMSLASRPIIIPDTSKVIIRAVQKNKREIK
CCCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEEECCCHHHHHHHHHCCCCEEE
LWKDGSKCMTVKENDYCEINKGTKPCKMIKFNKSISYYITLIKKLDWKGNLSLKNNQLN
EEECCCEEEEEECCCCEEECCCCCCEEEEEECCCCEEEEEEEHHCCCCCCCEECCCCCC
>Mature Secondary Structure
MLIIYRSNSSVAKEASIFCEKALKDRNIKSKSIGSDFDTKKIEQYLFNLPSLPDLLIVLG
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEC
GDGTVLKSANALVHYDIPLLSFNIGGNLGFLTQEKDFLFDKSFIKILEKEEFTIDLRNRL
CCCCEEECCCEEEEECCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCE
HCEVYSKENLGVRKIIKSYDALNDFYFKSVEEDISPTNQIQIEIDNEKVNEYKGDGLIIS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEE
SSTGSTAYSMAAGGPIVHPSINAFVINPICPMSLASRPIIIPDTSKVIIRAVQKNKREIK
CCCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEEECCCHHHHHHHHHCCCCEEE
LWKDGSKCMTVKENDYCEINKGTKPCKMIKFNKSISYYITLIKKLDWKGNLSLKNNQLN
EEECCCEEEEEECCCCEEECCCCCCEEEEEECCCCEEEEEEEHHCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]