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The map label for this gene is trpA [H]

Identifier: 161353817

GI number: 161353817

Start: 548931

End: 549758

Strand: Direct

Name: trpA [H]

Synonym: A9601_06281

Alternate gene names: 161353817

Gene position: 548931-549758 (Clockwise)

Preceding gene: 123968162

Following gene: 161353816

Centisome position: 32.87

GC content: 33.7

Gene sequence:

>828_bases
ATGCAAATTAATAAAAATGAATCTTTATCTAAGGTAGATGAAATGTTTAGTGAGTTAAAAAATAAAAAAAAATTTGCTTT
AATGCCTTTTATAATGGCTGGGGATCCCAATATTGAAATAACGTCTGAGATATTATTAAAGTTACAAGAAAATGGAGCTG
ACCTTGTTGAATTAGGCATCCCGTATAGTGACCCACTTGCAGATGGTCCTGTTATTCAATTGGCGGCCTCTCGCGCCTTA
AAGTCAGGTACTAGCTTAAGAAAAGTAATTAAACTTTTAGAGTCTTTAAAAGGTAAATTAAATATTCCCATCATCCTTTT
TTCTTACTTGAATCCATTACTATGTTTTGGCTTTGAAAGGTTTTGTGAGATGGCATCTGATGCTGGAGTTTCTGGACTAA
TAGTTCCTGACCTCCCTTTGGAGGAAGCTTATAAATTTTCTAAAATAGTTAGTCACCATTCTATGGACTTAATTTTATTA
GTAGCGCCAACTACTCCTTTTGAAAGAATGAAACAAATATCAAATCATACAAAAGGCTTTACTTATTTAGTAAGTGTTAC
GGGTGTCACTGGTGAGAGAAATAAAATGGAAAATAGAGTAGAAAATCTTATTGCTAAATTAAAAGATGTAAATAGCAATC
CAATTGCTGTTGGTTTTGGAATATCAACTCCAGAACACGTTAATAAAGTTCGTGAGTGGGGAGCAGATGGAGTAATTATT
GGGAGTGCATTTGTAAAAAGAATCTCGAGCTTAGGTGAAAAGGATGTTGTCGATCACGTTGGTGAGTTTTGTAAAGAAAT
GCGTTTAGCTGCGGATCAAAAAAAATAA

Upstream 100 bases:

>100_bases
TCATTGTCAAAGGAAGAGCAGCAAAAACTTATGGAAGATTTAGAGAAAAATCCATAAATTTTTCATAGAAAAATATCTAA
AAATTATGAAAGATAACAAA

Downstream 100 bases:

>100_bases
ATACAAATATAATTTATTAATTAAAAGAATTATTTATAGAAAATTAATTAAAATTTTATAACCAATTTATTTTTTTTATC
TTTAAGATTCTTCTGTAGGT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQINKNESLSKVDEMFSELKNKKKFALMPFIMAGDPNIEITSEILLKLQENGADLVELGIPYSDPLADGPVIQLAASRAL
KSGTSLRKVIKLLESLKGKLNIPIILFSYLNPLLCFGFERFCEMASDAGVSGLIVPDLPLEEAYKFSKIVSHHSMDLILL
VAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKDVNSNPIAVGFGISTPEHVNKVREWGADGVII
GSAFVKRISSLGEKDVVDHVGEFCKEMRLAADQKK

Sequences:

>Translated_275_residues
MQINKNESLSKVDEMFSELKNKKKFALMPFIMAGDPNIEITSEILLKLQENGADLVELGIPYSDPLADGPVIQLAASRAL
KSGTSLRKVIKLLESLKGKLNIPIILFSYLNPLLCFGFERFCEMASDAGVSGLIVPDLPLEEAYKFSKIVSHHSMDLILL
VAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKDVNSNPIAVGFGISTPEHVNKVREWGADGVII
GSAFVKRISSLGEKDVVDHVGEFCKEMRLAADQKK
>Mature_275_residues
MQINKNESLSKVDEMFSELKNKKKFALMPFIMAGDPNIEITSEILLKLQENGADLVELGIPYSDPLADGPVIQLAASRAL
KSGTSLRKVIKLLESLKGKLNIPIILFSYLNPLLCFGFERFCEMASDAGVSGLIVPDLPLEEAYKFSKIVSHHSMDLILL
VAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKDVNSNPIAVGFGISTPEHVNKVREWGADGVII
GSAFVKRISSLGEKDVVDHVGEFCKEMRLAADQKK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=241, Percent_Identity=39.0041493775934, Blast_Score=167, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321412, Length=266, Percent_Identity=37.218045112782, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 30349; Mature: 30349

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINKNESLSKVDEMFSELKNKKKFALMPFIMAGDPNIEITSEILLKLQENGADLVELGI
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECC
PYSDPLADGPVIQLAASRALKSGTSLRKVIKLLESLKGKLNIPIILFSYLNPLLCFGFER
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FCEMASDAGVSGLIVPDLPLEEAYKFSKIVSHHSMDLILLVAPTTPFERMKQISNHTKGF
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCE
TYLVSVTGVTGERNKMENRVENLIAKLKDVNSNPIAVGFGISTPEHVNKVREWGADGVII
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEE
GSAFVKRISSLGEKDVVDHVGEFCKEMRLAADQKK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQINKNESLSKVDEMFSELKNKKKFALMPFIMAGDPNIEITSEILLKLQENGADLVELGI
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECC
PYSDPLADGPVIQLAASRALKSGTSLRKVIKLLESLKGKLNIPIILFSYLNPLLCFGFER
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FCEMASDAGVSGLIVPDLPLEEAYKFSKIVSHHSMDLILLVAPTTPFERMKQISNHTKGF
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCE
TYLVSVTGVTGERNKMENRVENLIAKLKDVNSNPIAVGFGISTPEHVNKVREWGADGVII
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEE
GSAFVKRISSLGEKDVVDHVGEFCKEMRLAADQKK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]