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The map label for this gene is acnB [H]

Identifier: 123969442

GI number: 123969442

Start: 1647443

End: 1650016

Strand: Direct

Name: acnB [H]

Synonym: A9601_19101

Alternate gene names: 123969442

Gene position: 1647443-1650016 (Clockwise)

Preceding gene: 123969440

Following gene: 123969443

Centisome position: 98.66

GC content: 34.62

Gene sequence:

>2574_bases
ATGAAGAATTTGGAAACATTGCTAAAAGATTACGCAGATCACGTAGCGGAAAGAGCTGCCAAAGGTATACCTCCTTTACC
TTTAAATGCTGAGCAAACAAATTGTATTACAAAATTATTGGAACAAGATAGTAATTACGATTCTTCTTATTTACTTGATT
TACTTCTAAATAGAGTTCCACCAGGAGTCGATGAGGCTGCTTATGTAAAAGCAAGCTGGCTTACAGCTATTGTTAATTCC
GAAAAATATTGCAAATTAATTAATCCCGAAAAAGCAATTGAAATACTAGGAACAATGATAGGCGGATATAATGTAAATTC
TCTGGTTGAAATACTGAAAGGGGAAAATAGTTTACTTGCTAAAAAAGCGGCAGAAGTTTTAAAAAATATCATTCTTGTTT
ACGATTCAGCTAATGAAATCTATGAATTGTCACAAAATAATTTTTATGCAAAAGAGGTTGTAAATAGTTGGGCAAATGCA
GAATGGTTCAAAAATAAAAAAGTTCTTGAAAAAGAGATTACTTGTTTGGTGTTTAAAGTTGACGGTGAAACAAATACAGA
CGACTTATCTCCAGCTGTACATGCAACAACACGCCCGGATATTCCGATGCACGCATTAGCTATGTTGGAATTCAAAAAAC
CTGATGGACTAAAGATTCTTGATAATTTAAAAAAACAAAATTTACCAATAGCATATGTTGGAGATGTTGTTGGAACAGGG
AGTTCTAGAAAATCTGCTATTAATTCACTCATTTGGCATATAGGAGAAGATATTGCTTTTGTTCCAAACAAAAAAACAGG
TGGAATAATAATTGGCAGCAAAATAGCCCCAATTTTCTTTAATACTGCACAAGATTCAGGAGCTTTACCAATAGAAGCTG
ACGTATCTCAAATGAAAACAGGAGATGTTATTAAGATATATCCTTATGCAGGCATTATTAAAAAAGTTGAAAAAGATTCA
AATACTGAAGAATTAATAAGCAAATTCGACTTGTATCCATCAACTCTTAGTGATGAGATTCAAGCTGGCGGAAGAATTAA
TCTTATGATTGGTAGATCTCTTACAGATAAAATTAGAAATAAATTAAATTATCAACCTAGTGAAATATTTACTAGGCCAC
AAAATCCAACTGAAAGTAGTGCCGGATTTACTCAAGCTCAAAAAATAGTAGGCAAAGCATGCGGTTTAGAGGGAGTAAGG
CCAGGAATGACCTGTGAGCCAATTATGACCACAGTTGGTAGTCAAGATACTACTGGGCCAATGACTAGAGATGAACTAAA
AGAACTAGCTTGTTTAGGATTTACTGCAGATTTAGTGATGCAAAGTTTTTGTCATACAGCTGCGTATCCTAAACCAGTAG
ATCTAGTTACCCATAAAGAATTACCTGATTTTATATCACAAAGAGGTGGAGTAGCTCTTAAGCCTGGAGACGGCATAATT
CATAGCTGGCTTAATAGAATGCTTCTCCCAGATACTGTTGGCACAGGCGGAGATAGTCATACTAGATTTCCTCTTGGCAT
TTCATTTCCTGGAGGCTCGGGCATTGTTGCCTTTGCCGCTGCAATAGGATCAATGCCATTAAATATGCCAGAATCTGTAC
TGGTTAAATTTAAGGGAGAATTATTACCAGGAATCACTCTCAGAGATTTAGTAAATGCAATCCCTCTTTTCGCAATTAAA
AAAGGGCTCTTAACTGTTGAGAAAGCAAATAAGAAAAATATATTCAACGGGAAAATTATGGAAATTGAGGGATTACCAAA
CCTAAAACTTGAACAAGCTTTTGAACTTACTGATGCTACTGCAGAACGCTCATGCGCTGGTAGTACCATACTTTTATCCC
AAGAAACTGTAAAAGAATACTTAAGAAGCAATATTTGCCTGCTAGAAAAAATGATTGAAAGCAATTATGAAGATTCAAAA
TCAATTTCAAGAAGAATAAATGATATGAAAAATTGGTTAAAACAACCATCATTGATTCAACCAGATTCAAACGCTCAGTA
TAAAGAAATCATTGAAATTGATTTAGCAAAAGTAACACAACCTATAGTTGCTTGCCCAAACGATCCGGATAATGTAAAAG
AAATCACTGATGTTGCAAATACAAATATTGACGAAGTTTTTATAGGTTCTTGCATGACTAATATTGGCCATTACAGGGCA
GCTGCGAAAGTTCTTGAAGGTGTACAAAATTTAAAAGCTAAATTATGGATTTGTCCACCAACAAAAATGGATGAAGAAAC
TCTAAAAGCTGAAGGTTACTATAAAATTTTCGAAGATTGTGGTGCAAGGTTAGAGTTACCAGGCTGTTCTTTATGTATGG
GTAATCAAGCCAGAGTAGATGAAGGATCCGTAGTATTTTCTACTAGTACAAGAAATTTTGACAATAGACTTGGCAAGAAT
GCACAAGTCTTTTTAGGCAGCGCCGAATTAGCAGCAGTTTGCGCACTGCTTGGCAAAATACCTGAAGTTGAAGAATATCA
GGATATTACTAAAAATAAAATTAATCCATATTCGGATGAACTTTATCGCTATCTTCAATTTGATGAAATACAAGATTTCA
GCTTGACAAAGTAA

Upstream 100 bases:

>100_bases
CATTGATTAATTAAGAAGTTTGAATATTTAAAAAAGCATTTATGAATACTCTCCTTTTCAATATTAAAAATAACAATAGA
TTAGGCATTAAACCTTACTA

Downstream 100 bases:

>100_bases
TCATGGACTATGCCAAACTTTATAAAAGATAATATTCAAAAAACAAGTAATAATTCTTCTCGTAGCATCAAAAAATTATT
AAAACAAAGATCTCTGGTTG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKNLETLLKDYADHVAERAAKGIPPLPLNAEQTNCITKLLEQDSNYDSSYLLDLLLNRVPPGVDEAAYVKASWLTAIVNS
EKYCKLINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNFYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKKPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAFVPNKKTGGIIIGSKIAPIFFNTAQDSGALPIEADVSQMKTGDVIKIYPYAGIIKKVEKDS
NTEELISKFDLYPSTLSDEIQAGGRINLMIGRSLTDKIRNKLNYQPSEIFTRPQNPTESSAGFTQAQKIVGKACGLEGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKANKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVKEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKQPSLIQPDSNAQYKEIIEIDLAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKAKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIQDFSLTK

Sequences:

>Translated_857_residues
MKNLETLLKDYADHVAERAAKGIPPLPLNAEQTNCITKLLEQDSNYDSSYLLDLLLNRVPPGVDEAAYVKASWLTAIVNS
EKYCKLINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNFYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKKPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAFVPNKKTGGIIIGSKIAPIFFNTAQDSGALPIEADVSQMKTGDVIKIYPYAGIIKKVEKDS
NTEELISKFDLYPSTLSDEIQAGGRINLMIGRSLTDKIRNKLNYQPSEIFTRPQNPTESSAGFTQAQKIVGKACGLEGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKANKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVKEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKQPSLIQPDSNAQYKEIIEIDLAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKAKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIQDFSLTK
>Mature_857_residues
MKNLETLLKDYADHVAERAAKGIPPLPLNAEQTNCITKLLEQDSNYDSSYLLDLLLNRVPPGVDEAAYVKASWLTAIVNS
EKYCKLINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNFYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKKPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAFVPNKKTGGIIIGSKIAPIFFNTAQDSGALPIEADVSQMKTGDVIKIYPYAGIIKKVEKDS
NTEELISKFDLYPSTLSDEIQAGGRINLMIGRSLTDKIRNKLNYQPSEIFTRPQNPTESSAGFTQAQKIVGKACGLEGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKANKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVKEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKQPSLIQPDSNAQYKEIIEIDLAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKAKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIQDFSLTK

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=431, Percent_Identity=25.0580046403712, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI2367097, Length=860, Percent_Identity=59.1860465116279, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=396, Percent_Identity=24.7474747474747, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=26.7156862745098, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=407, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94320; Mature: 94320

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLETLLKDYADHVAERAAKGIPPLPLNAEQTNCITKLLEQDSNYDSSYLLDLLLNRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGVDEAAYVKASWLTAIVNSEKYCKLINPEKAIEILGTMIGGYNVNSLVEILKGENSLLA
CCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
KKAAEVLKNIILVYDSANEIYELSQNNFYAKEVVNSWANAEWFKNKKVLEKEITCLVFKV
HHHHHHHHHHEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
DGETNTDDLSPAVHATTRPDIPMHALAMLEFKKPDGLKILDNLKKQNLPIAYVGDVVGTG
CCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHCCC
SSRKSAINSLIWHIGEDIAFVPNKKTGGIIIGSKIAPIFFNTAQDSGALPIEADVSQMKT
CHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCHHHCCC
GDVIKIYPYAGIIKKVEKDSNTEELISKFDLYPSTLSDEIQAGGRINLMIGRSLTDKIRN
CCEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCHHHHHHHH
KLNYQPSEIFTRPQNPTESSAGFTQAQKIVGKACGLEGVRPGMTCEPIMTTVGSQDTTGP
HCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LLPGITLRDLVNAIPLFAIKKGLLTVEKANKKNIFNGKIMEIEGLPNLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AERSCAGSTILLSQETVKEYLRSNICLLEKMIESNYEDSKSISRRINDMKNWLKQPSLIQ
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
PDSNAQYKEIIEIDLAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
CCCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAKVLEGVQNLKAKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVD
HHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHH
LYRYLQFDEIQDFSLTK
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKNLETLLKDYADHVAERAAKGIPPLPLNAEQTNCITKLLEQDSNYDSSYLLDLLLNRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGVDEAAYVKASWLTAIVNSEKYCKLINPEKAIEILGTMIGGYNVNSLVEILKGENSLLA
CCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
KKAAEVLKNIILVYDSANEIYELSQNNFYAKEVVNSWANAEWFKNKKVLEKEITCLVFKV
HHHHHHHHHHEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
DGETNTDDLSPAVHATTRPDIPMHALAMLEFKKPDGLKILDNLKKQNLPIAYVGDVVGTG
CCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHCCC
SSRKSAINSLIWHIGEDIAFVPNKKTGGIIIGSKIAPIFFNTAQDSGALPIEADVSQMKT
CHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCHHHCCC
GDVIKIYPYAGIIKKVEKDSNTEELISKFDLYPSTLSDEIQAGGRINLMIGRSLTDKIRN
CCEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCHHHHHHHH
KLNYQPSEIFTRPQNPTESSAGFTQAQKIVGKACGLEGVRPGMTCEPIMTTVGSQDTTGP
HCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LLPGITLRDLVNAIPLFAIKKGLLTVEKANKKNIFNGKIMEIEGLPNLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AERSCAGSTILLSQETVKEYLRSNICLLEKMIESNYEDSKSISRRINDMKNWLKQPSLIQ
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
PDSNAQYKEIIEIDLAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
CCCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAKVLEGVQNLKAKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVD
HHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHH
LYRYLQFDEIQDFSLTK
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]