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The map label for this gene is gidA [H]

Identifier: 123969416

GI number: 123969416

Start: 1619587

End: 1621554

Strand: Direct

Name: gidA [H]

Synonym: A9601_18841

Alternate gene names: 123969416

Gene position: 1619587-1621554 (Clockwise)

Preceding gene: 123969415

Following gene: 123969417

Centisome position: 96.99

GC content: 33.08

Gene sequence:

>1968_bases
ATGCAAGATCATAACTCAACAAATGAATCTTTTGACGTCATCGTTATTGGAGGAGGACATGCAGGATGCGAAGCCGCTAT
AACAACAGCAAAATTAGGTTTTTCTACTGCCTTATTTACAATCAATCTAGATAGGATTGCCTGGCAACCGTGTAATCCTG
CTGTTGGGGGACCAGCAAAAAGTCAGTTGGTTCATGAAGTTGATGCATTAGGTGGAATTATTGGTAAATTAGCCGATGAA
ACAGCTATTCAAAAAAGAATATTAAATGCAAGTAGAGGCCCAGCTGTATGGGCGTTAAGAGCTCAGACAGATAAAAGAGA
ATACTCAAAAAAAATGATCGAAATACTACAAAATACAGATAATTTATCTTTAAAAGAAGCAATGATTACTGAACTGGATA
TTGCAAAAACTGAACAAATTGGATTGAACTCAAAAAAAATCTTAAAAAAAAGAATAAAGGGTGTAAAAACATTCTTTGGT
AGTTATTATTCCGCAAGATCAGTTATCATCACAGCTGGTACATTCTTAGAAGGAAGAATATGGATAGGAAATAAATCAAT
GTCAGCTGGCAGATCGGGCGAACAAGCAGCACAAGGTCTTACTCAGAATTTACACGAAATTGGTATCAAAACAGAACGTT
TAAAAACAGGTACTCCAGCAAGAGTTGATAAAAGAAGTATCATTTTTGATGAATTAGATGTTCAACCAAGTACTGCAGTA
GATAAATATTTTTCGTTTGACCCAGATATAAAAAATAATATGCCCCAAGTTAGTTGTCACATCACAAGAACAACTACCAA
AACCCATCAACTTATTAGAGATAATTTACATTTAACTCCCATTTACGGCGGTTTTATTGATAGTAAAGGGCCAAGATATT
GTCCATCAATTGAAGATAAAATCGTCAAATTTGCTAATAAAGAATCACATCAAATTTTCTTAGAACCAGAAGGAATTAAT
ACCCCTGAAATATATGTACAAGGATTTTCTACAGGTTTACCCGAAAATATTCAATTAGAACTTTTAAGGACCTTACCTGG
ATTAGGTGAATGTAAAATGTTGCGACCAGCATATGCTGTGGAGTATGACTATATACCTGCAACACAGCTCCAAACATCAC
TCGAAACGAAAGAAATTGAATATTTATTTAGCGCCGGACAAATTAATGGCACTACTGGTTATGAAGAAGCAGCAGCACAA
GGATTAGTTGCAGGAGTCAATGCGACAAGAAAACTAAACAAAAAAGATCCAATAATCTTCTCCAGAGAAAGCAGTTATAT
AGGAACAATGATCAATGATTTAATTACAAAAGATCTCAAAGAACCATATAGAGTTCTCACTAGTAGGAGTGAATATAGGT
TAACTCTCAGAGGAGATAATGCAGATAGAAGATTAACACCATTAGGTTATCAAATAGGGCTAATTAATGAGAAAAGATGG
TCGGCCTATCAAGAAAAGATGAACCTCCTCGAAGAAGAGAAATTTAGATTGAATAATACTCGTTTAAAAAATACCGATGA
AATATCAAAAAAAATAGAATTAGAAACGGGATCAAAAATCAAAGGCTCAACAACTTTGAAAGAACTCTTAAAAAGACCAA
ACTTTCATTATTCAGATCTAATTAAATATAATTTGAATGAAAGAAATCTAGGTTCTTCAATACAGGAAGGTGTTGAAATA
GATATTAAATACGAGGGTTACCTTAAAAGACAAAAAAACAACATAGAACAAATAAATCGTCAAAGCTGTAAATCTCTGCC
TCAAGAAATAAACTATGAAAAGATAGAGACATTATCTTTAGAAGCCAGAGAAAATTTGAATAAGATAAAGCCAAAAAATT
TTGGTGATGCTTCAAAAATTCCCGGTGTCAGCAAAGCTGATTTAACTGCATTACTTGTTTGGCTAAAAATAAGAGAAATA
AAAAAAGAAAAGGCAAATATTTTTGTCGAAAAAAAGTTATCATCTTAA

Upstream 100 bases:

>100_bases
TGTCACTAAACATAGAAATGGACCCGTAGGAACTGTTAAATTATTATTTGAACCTCAATTTACGAGATTTAGGAATTTAG
CTAATTAAATCGATCCTAAA

Downstream 100 bases:

>100_bases
AAGGATTCCGTCTGAGCACTGAATAATAAACTTTTTAAATCACCAAAAATTTTATGGGAAGAAAAAGCCTCTACTTTTTT
AGTGAAAAATTCATTACCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MQDHNSTNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKKMIEILQNTDNLSLKEAMITELDIAKTEQIGLNSKKILKKRIKGVKTFFG
SYYSARSVIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTQNLHEIGIKTERLKTGTPARVDKRSIIFDELDVQPSTAV
DKYFSFDPDIKNNMPQVSCHITRTTTKTHQLIRDNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFANKESHQIFLEPEGIN
TPEIYVQGFSTGLPENIQLELLRTLPGLGECKMLRPAYAVEYDYIPATQLQTSLETKEIEYLFSAGQINGTTGYEEAAAQ
GLVAGVNATRKLNKKDPIIFSRESSYIGTMINDLITKDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGYQIGLINEKRW
SAYQEKMNLLEEEKFRLNNTRLKNTDEISKKIELETGSKIKGSTTLKELLKRPNFHYSDLIKYNLNERNLGSSIQEGVEI
DIKYEGYLKRQKNNIEQINRQSCKSLPQEINYEKIETLSLEARENLNKIKPKNFGDASKIPGVSKADLTALLVWLKIREI
KKEKANIFVEKKLSS

Sequences:

>Translated_655_residues
MQDHNSTNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKKMIEILQNTDNLSLKEAMITELDIAKTEQIGLNSKKILKKRIKGVKTFFG
SYYSARSVIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTQNLHEIGIKTERLKTGTPARVDKRSIIFDELDVQPSTAV
DKYFSFDPDIKNNMPQVSCHITRTTTKTHQLIRDNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFANKESHQIFLEPEGIN
TPEIYVQGFSTGLPENIQLELLRTLPGLGECKMLRPAYAVEYDYIPATQLQTSLETKEIEYLFSAGQINGTTGYEEAAAQ
GLVAGVNATRKLNKKDPIIFSRESSYIGTMINDLITKDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGYQIGLINEKRW
SAYQEKMNLLEEEKFRLNNTRLKNTDEISKKIELETGSKIKGSTTLKELLKRPNFHYSDLIKYNLNERNLGSSIQEGVEI
DIKYEGYLKRQKNNIEQINRQSCKSLPQEINYEKIETLSLEARENLNKIKPKNFGDASKIPGVSKADLTALLVWLKIREI
KKEKANIFVEKKLSS
>Mature_655_residues
MQDHNSTNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAKSQLVHEVDALGGIIGKLADE
TAIQKRILNASRGPAVWALRAQTDKREYSKKMIEILQNTDNLSLKEAMITELDIAKTEQIGLNSKKILKKRIKGVKTFFG
SYYSARSVIITAGTFLEGRIWIGNKSMSAGRSGEQAAQGLTQNLHEIGIKTERLKTGTPARVDKRSIIFDELDVQPSTAV
DKYFSFDPDIKNNMPQVSCHITRTTTKTHQLIRDNLHLTPIYGGFIDSKGPRYCPSIEDKIVKFANKESHQIFLEPEGIN
TPEIYVQGFSTGLPENIQLELLRTLPGLGECKMLRPAYAVEYDYIPATQLQTSLETKEIEYLFSAGQINGTTGYEEAAAQ
GLVAGVNATRKLNKKDPIIFSRESSYIGTMINDLITKDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGYQIGLINEKRW
SAYQEKMNLLEEEKFRLNNTRLKNTDEISKKIELETGSKIKGSTTLKELLKRPNFHYSDLIKYNLNERNLGSSIQEGVEI
DIKYEGYLKRQKNNIEQINRQSCKSLPQEINYEKIETLSLEARENLNKIKPKNFGDASKIPGVSKADLTALLVWLKIREI
KKEKANIFVEKKLSS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=649, Percent_Identity=39.1371340523883, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI19882217, Length=674, Percent_Identity=37.6854599406528, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI183227703, Length=689, Percent_Identity=36.8650217706821, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=43.5612082670906, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17534255, Length=634, Percent_Identity=36.2776025236593, Blast_Score=397, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321202, Length=629, Percent_Identity=40.8585055643879, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24658174, Length=656, Percent_Identity=38.2621951219512, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 73590; Mature: 73590

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDHNSTNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAK
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEHHEEECCCCCCCCCCHH
SQLVHEVDALGGIIGKLADETAIQKRILNASRGPAVWALRAQTDKREYSKKMIEILQNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCC
NLSLKEAMITELDIAKTEQIGLNSKKILKKRIKGVKTFFGSYYSARSVIITAGTFLEGRI
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
WIGNKSMSAGRSGEQAAQGLTQNLHEIGIKTERLKTGTPARVDKRSIIFDELDVQPSTAV
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCCHHH
DKYFSFDPDIKNNMPQVSCHITRTTTKTHQLIRDNLHLTPIYGGFIDSKGPRYCPSIEDK
HHHCCCCCCHHCCCCEEEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHH
IVKFANKESHQIFLEPEGINTPEIYVQGFSTGLPENIQLELLRTLPGLGECKMLRPAYAV
HHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEECCHHEE
EYDYIPATQLQTSLETKEIEYLFSAGQINGTTGYEEAAAQGLVAGVNATRKLNKKDPIIF
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEE
SRESSYIGTMINDLITKDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGYQIGLINEKRW
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEEECCCHHH
SAYQEKMNLLEEEKFRLNNTRLKNTDEISKKIELETGSKIKGSTTLKELLKRPNFHYSDL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCCHHHH
IKYNLNERNLGSSIQEGVEIDIKYEGYLKRQKNNIEQINRQSCKSLPQEINYEKIETLSL
EEECCCCCCCCCHHHCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHEEEH
EARENLNKIKPKNFGDASKIPGVSKADLTALLVWLKIREIKKEKANIFVEKKLSS
HHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
>Mature Secondary Structure
MQDHNSTNESFDVIVIGGGHAGCEAAITTAKLGFSTALFTINLDRIAWQPCNPAVGGPAK
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEHHEEECCCCCCCCCCHH
SQLVHEVDALGGIIGKLADETAIQKRILNASRGPAVWALRAQTDKREYSKKMIEILQNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCC
NLSLKEAMITELDIAKTEQIGLNSKKILKKRIKGVKTFFGSYYSARSVIITAGTFLEGRI
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
WIGNKSMSAGRSGEQAAQGLTQNLHEIGIKTERLKTGTPARVDKRSIIFDELDVQPSTAV
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCCHHH
DKYFSFDPDIKNNMPQVSCHITRTTTKTHQLIRDNLHLTPIYGGFIDSKGPRYCPSIEDK
HHHCCCCCCHHCCCCEEEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHH
IVKFANKESHQIFLEPEGINTPEIYVQGFSTGLPENIQLELLRTLPGLGECKMLRPAYAV
HHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEECCHHEE
EYDYIPATQLQTSLETKEIEYLFSAGQINGTTGYEEAAAQGLVAGVNATRKLNKKDPIIF
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEE
SRESSYIGTMINDLITKDLKEPYRVLTSRSEYRLTLRGDNADRRLTPLGYQIGLINEKRW
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEEECCCHHH
SAYQEKMNLLEEEKFRLNNTRLKNTDEISKKIELETGSKIKGSTTLKELLKRPNFHYSDL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCCHHHH
IKYNLNERNLGSSIQEGVEIDIKYEGYLKRQKNNIEQINRQSCKSLPQEINYEKIETLSL
EEECCCCCCCCCHHHCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHEEEH
EARENLNKIKPKNFGDASKIPGVSKADLTALLVWLKIREIKKEKANIFVEKKLSS
HHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA